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PAthogenisity Island DataBase (PAI DB)

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Homologs of hrpZ1 gene of NC_004578_P1

protein-level

Hit (GenBank Accn)

Product

Source Genome

Eval.

Ident.(%)

1) hrpZ1
( NP_791209.1 )

type III restriction system endonuclease

NC_004578
(Pseudomonas syringae DC3000)

2e-111

100

2) Psyr_1193
( YP_234285.1 )

type III restriction system endonuclease

NC_007005
(Pseudomonas syringae B728a)

3e-64

3) hrpZ1
( YP_273532.1 )

type III restriction system endonuclease

NC_005773
(Pseudomonas syringae 1448A)

3e-57

• Sequence Alignments

1) hrpZ1 in NC_004578


> NC_004578_P1|27|G19|28868590|NP_791209.1|Pseudomonas_syringae_pv._tomato_str._DC3000|+|22284..23396|hrpZ1|PSPTO_1382|type 
III restriction system endonuclease
Length=370

 Score =  395 bits (1486),  Expect = 2e-111, Method: Compositional matrix adjust.
 Identities = 370/370 (100%), Positives = 370/370 (100%), Gaps = 0/370 (0%)

Query  1    MQALNSISSLQTSASLFPVSLNSDVSANTSTSSKELKAVIDQLVQALTQSGQLDETSPLG  60
            MQALNSISSLQTSASLFPVSLNSDVSANTSTSSKELKAVIDQLVQALTQSGQLDETSPLG
Sbjct  1    MQALNSISSLQTSASLFPVSLNSDVSANTSTSSKELKAVIDQLVQALTQSGQLDETSPLG  60

Query  61   KMLAKAMAADGKSANSIDDITASLDKLIHEKLGDNFgasagigaggggggiggagsgsgv  120
            KMLAKAMAADGKSANSIDDITASLDKLIHEKLGDNFGASAGIGAGGGGGGIGGAGSGSGV
Sbjct  61   KMLAKAMAADGKSANSIDDITASLDKLIHEKLGDNFGASAGIGAGGGGGGIGGAGSGSGV  120

Query  121  ggglssdagagqsDLMSQVLNGLGKAVLDDLLTPSGEGGTTFSSDDMPTLEKVAQFMDDN  180
            GGGLSSDAGAGQSDLMSQVLNGLGKAVLDDLLTPSGEGGTTFSSDDMPTLEKVAQFMDDN
Sbjct  121  GGGLSSDAGAGQSDLMSQVLNGLGKAVLDDLLTPSGEGGTTFSSDDMPTLEKVAQFMDDN  180

Query  181  KAQFPTRDGGSWMNELKEDNGLDAQETAQFRSALDVIGQQLGQQQGDASGVTSGGGLGSP  240
            KAQFPTRDGGSWMNELKEDNGLDAQETAQFRSALDVIGQQLGQQQGDASGVTSGGGLGSP
Sbjct  181  KAQFPTRDGGSWMNELKEDNGLDAQETAQFRSALDVIGQQLGQQQGDASGVTSGGGLGSP  240

Query  241  VSDSSLGNPAIDANTGPAANGNASVDVGQLIGQLIDRglqsvssggglgTPVDNSTQPTG  300
            VSDSSLGNPAIDANTGPAANGNASVDVGQLIGQLIDRGLQSVSSGGGLGTPVDNSTQPTG
Sbjct  241  VSDSSLGNPAIDANTGPAANGNASVDVGQLIGQLIDRGLQSVSSGGGLGTPVDNSTQPTG  300

Query  301  GTPAANPTGNVSNqdlgqllsgllqrglEATLQDAGNTGadlqssaaqvaaqlINALLQG  360
            GTPAANPTGNVSNQDLGQLLSGLLQRGLEATLQDAGNTGADLQSSAAQVAAQLINALLQG
Sbjct  301  GTPAANPTGNVSNQDLGQLLSGLLQRGLEATLQDAGNTGADLQSSAAQVAAQLINALLQG  360

Query  361  TNNQTNQAVA  370
            TNNQTNQAVA
Sbjct  361  TNNQTNQAVA  370

2) Psyr_1193 in NC_007005


> NC_004578_P1|27|G19|28868590|NP_791209.1|Pseudomonas_syringae_pv._tomato_str._DC3000|+|22284..23396|hrpZ1|PSPTO_1382|type 
III restriction system endonuclease
Length=370

 Score =  238 bits (889),  Expect = 3e-64, Method: Compositional matrix adjust.
 Identities = 188/346 (55%), Positives = 251/346 (73%), Gaps = 37/346 (10%)

Query  27   TTGASSSSKALQEVVVKLAEELMRNGQLDDSSPLGKLLAKSMAADGKAGGGIEDVIAALD  86
            ++ +S+SSK L+ V+ +L + L ++GQLD++SPLGK+LAK+MAADGK++ +I+D+ A+LD
Sbjct  26   SANTSTSSKELKAVIDQLVQALTQSGQLDETSPLGKMLAKAMAADGKSANSIDDITASLD  85

Query  87   KLIHEKLGDNFGASA------------------------DNASGTGQQDLMTQVLNGLAK  122
            KLIHEKLGDNFGASA                         + +G+GQ DLM+QVLNGL+K
Sbjct  86   KLIHEKLGDNFGASAGIGAGGGGGGIGGAGSGSGVGGGLSSDAGAGQSDLMSQVLNGLGK  145

Query  123  SMLDDLLTKQ-DGGTSFSEDDMPMLNKIAQFMDDNPAQFPKPDSGSWVNELKEDNFLDGD  181
            ++LDDLLT   +GGT+FS DDMP L+K+AQFMDDN AQFP  D+GSW+NELKEDN LD++
Sbjct  146  AVLDDLLTPSGEGGTTFSSDDMPTLEKVAQFMDDNKAQFPTRDGGSWMNELKEDNGLDAQ  205

Query  182  ETAAFRSALDIIGQQLGNQQSgtgglagtggglgtPssfssnssGVKGDPLIDANTGPGD  241
            ETA FRSALD+IGQQ   QQ G ++   +GGGLG+P S SS      G+P IDANTGP+ 
Sbjct  206  ETAQFRSALDVIGQQ-LGQQQGDASGVTSGGGLGSPVSDSSL-----GNPAIDANTGPAA  259

Query  242  SGTTSGEAGQLIGELIDRGLQSVLAGGGLGTPVNT---PQTGTAANggqsaqdldqllgg  298
            +G +S + GQLIG+LIDRGLQSV +GGGLGTPV+    P  GT+A    +    ++L   
Sbjct  260  NGNASVDVGQLIGQLIDRGLQSVSSGGGLGTPVDNSTQPTGGTPAANPTGNVSNQDLGQL  319

Query  299  lllkgl---EATLKDAGQTATDVQSSAAQIATLLVSTLLQGTRNQA  341
            L        EATL+DAG+T++D+QSSAAQ+A+ L+++LLQGT NQ+
Sbjct  320  LSGLLQRGLEATLQDAGNTGADLQSSAAQVAAQLINALLQGTNNQT  365

3) hrpZ1 in NC_005773


> NC_004578_P1|27|G19|28868590|NP_791209.1|Pseudomonas_syringae_pv._tomato_str._DC3000|+|22284..23396|hrpZ1|PSPTO_1382|type 
III restriction system endonuclease
Length=370

 Score =  215 bits (801),  Expect = 3e-57, Method: Compositional matrix adjust.
 Identities = 201/340 (60%), Positives = 259/340 (77%), Gaps = 39/340 (11%)

Query  35   RALQEVIAQLAQELTHNGQLDESSPlgkllgkamaasgkaggglEDIKAALDTLIHEKLG  94
            + L+ VI QL Q LT++GQLDE+SPLGK+L+KAMAA GK++ +++DI A+LD LIHEKLG
Sbjct  34   KELKAVIDQLVQALTQSGQLDETSPLGKMLAKAMAADGKSANSIDDITASLDKLIHEKLG  93

Query  95   DNFGASA------------------------DNASDTGQHDLMTQVLNGLAKSMLNDLLT  130
            DNFGASA                         + +++GQ DLM+QVLNGL+K++L+DLLT
Sbjct  94   DNFGASAGIGAGGGGGGIGGAGSGSGVGGGLSSDAGAGQSDLMSQVLNGLGKAVLDDLLT  153

Query  131  -KQDDGTRFSEDDMPMLKKIAEFMDDNPAQFPKPDSGSWVNELKEDNFLDGDETAQFRSA  189
               ++GT FS DDMP L K+A+FMDDN AQFP  D+GSW+NELKEDN LD++ETAQFRSA
Sbjct  154  PSGEGGTTFSSDDMPTLEKVAQFMDDNKAQFPTRDGGSWMNELKEDNGLDAQETAQFRSA  213

Query  190  LDIIGQQLGSQQNaagglagdssggglgsPVSNTENSPGSLGDPLIDANTGPASNSNSNG  249
            LD+IGQ      +  G+ +G +SGGGLGSPVS++     SLG+P IDANTGPA+N+N++ 
Sbjct  214  LDVIGQ---QLGQQQGDASGVTSGGGLGSPVSDS-----SLGNPAIDANTGPAANGNASV  265

Query  250  DVGQLIGELIDRGLQSVLAGGGLGTPV------STANTALVPGGEQPNQDlgqllggllq  303
            DVGQLIG+LIDRGLQSV +GGGLGTPV      + +  A  P G+ +NQDLGQLL+GLLQ
Sbjct  266  DVGQLIGQLIDRGLQSVSSGGGLGTPVDNSTQPTGGTPAANPTGNVSNQDLGQLLSGLLQ  325

Query  304  kglEATLQDAGQTGTGVQSSAAQVALLLVNMLLQSTKNQA  343
            +GLEATLQDAG+TG+++QSSAAQVA  L+N LLQ+T+NQ+
Sbjct  326  RGLEATLQDAGNTGADLQSSAAQVAAQLINALLQGTNNQT  365