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Homologs of hrpJ gene of AF232004

protein-level

Hit (GenBank Accn)

Product

Source Genome

Eval.

Ident.(%)

1) hrpJ
( NP_791230.1 )

type III secretion protein HrpJ

NC_004578
(Pseudomonas syringae DC3000)

2e-165

100

2) hrpJ
( YP_273552.1 )

type III secretion component protein HrcJ

NC_005773
(Pseudomonas syringae 1448A)

2e-141

3) Psyr_1216
( YP_234308.1 )

type III secretion protein HrpJ

NC_007005
(Pseudomonas syringae B728a)

1e-133

4) Pmen_0038
( YP_001185544.1 )

TyeA family type III secretion effector delivery regulator

NC_009439
(Pseudomonas mendocina ymp)

4e-62

• Sequence Alignments

1) hrpJ in NC_004578


> AF232004|10|G9|11276508|AAG33876.1|Pseudomonas_syringae_pv._tomato_DC3000|+|11358..12464|hrpJ|-|HrpJ
Length=368

 Score =  574 bits (2168),  Expect = 2e-165, Method: Compositional matrix adjust.
 Identities = 368/368 (100%), Positives = 368/368 (100%), Gaps = 0/368 (0%)

Query  1    MKIVAPPIMRILPVAPTRVVTPAAQPLPNADLHNSGTSPQQVSRFAAALIQHRRILIQRD  60
            MKIVAPPIMRILPVAPTRVVTPAAQPLPNADLHNSGTSPQQVSRFAAALIQHRRILIQRD
Sbjct  1    MKIVAPPIMRILPVAPTRVVTPAAQPLPNADLHNSGTSPQQVSRFAAALIQHRRILIQRD  60

Query  61   LIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQDDSSSDLKQVLDYTDGD  120
            LIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQDDSSSDLKQVLDYTDGD
Sbjct  61   LIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQDDSSSDLKQVLDYTDGD  120

Query  121  AAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKYGPRARAGINSAKAFARPNIDNK  180
            AAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKYGPRARAGINSAKAFARPNIDNK
Sbjct  121  AAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKYGPRARAGINSAKAFARPNIDNK  180

Query  181  RRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQFNIDLRDMRTAIADDLSAMTPSASH  240
            RRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQFNIDLRDMRTAIADDLSAMTPSASH
Sbjct  181  RRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQFNIDLRDMRTAIADDLSAMTPSASH  240

Query  241  EQLRTLMHGLNTARHVTTLLKGCEHLLGRMRNKNPDLTVDPPAFLKHLLTLTANGMNVNQ  300
            EQLRTLMHGLNTARHVTTLLKGCEHLLGRMRNKNPDLTVDPPAFLKHLLTLTANGMNVNQ
Sbjct  241  EQLRTLMHGLNTARHVTTLLKGCEHLLGRMRNKNPDLTVDPPAFLKHLLTLTANGMNVNQ  300

Query  301  TLQLTQHIGGKQLKHQLAFLNGlrpmllqlpillWRDMKSRQTALSNLLILMAELTTQEQ  360
            TLQLTQHIGGKQLKHQLAFLNGLRPMLLQLPILLWRDMKSRQTALSNLLILMAELTTQEQ
Sbjct  301  TLQLTQHIGGKQLKHQLAFLNGLRPMLLQLPILLWRDMKSRQTALSNLLILMAELTTQEQ  360

Query  361  KQLYGGLV  368
            KQLYGGLV
Sbjct  361  KQLYGGLV  368

2) hrpJ in NC_005773


> AF232004|10|G9|11276508|AAG33876.1|Pseudomonas_syringae_pv._tomato_DC3000|+|11358..12464|hrpJ|-|HrpJ
Length=368

 Score =  494 bits (1864),  Expect = 2e-141, Method: Compositional matrix adjust.
 Identities = 286/368 (78%), Positives = 333/368 (91%), Gaps = 1/368 (0%)

Query  1    MKIVAPPILPTHTVTPIRAVTPAARAIPGTLSSEKGTSSQQVSRFAAALVRNSRILRQRE  60
            MKIVAPPI+    V+P R VTPAA+++P +   + GTS+QQVSRFAAAL+++ RIL QR+
Sbjct  1    MKIVAPPIMRILPVAPTRVVTPAAQPLPNADLHNSGTSPQQVSRFAAALIQHRRILIQRD  60

Query  61   LIASRNALQSRAVKLGELYQLLMSANDTGLDNAARLLRKKLLQENDT-ELEQVLEFADGD  119
            LIAS NALQSRAVKLGELYQLLM+ +DTGLDNAAR+LRKKL+Q++ + +L QVL+++DGD
Sbjct  61   LIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQDDSSSDLKQVLDYTDGD  120

Query  120  AAKAHVVLQAARKQAEEDGAEGEYVALTQTLKQLRRKFGPHARAGINTARAFGRQNVDNK  179
            AAKAHVVLQAARKQAE DG  GE V LTQ LKQLRRK+GP+ARAGIN+A+AF+R N+DNK
Sbjct  121  AAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKYGPRARAGINSAKAFARPNIDNK  180

Query  180  RRTALRNLYGVAVSGQPNVTGLIEALINEQEETGDFDLNLRDMRRAIADDLSAITPSASH  239
            RR ALRNLY+VAVSGQPN+TGLIEAL+ EQ+E+G+F+++LRDMR AIADDLSA+TPSASH
Sbjct  181  RRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQFNIDLRDMRTAIADDLSAMTPSASH  240

Query  240  EQLRTLMHGLTTARHVTTLLRGCEHLLGRMRNKNPDLKVDPPAFLKHLLTLTANGMNVNQ  299
            EQLRTLMHGL TARHVTTLL+GCEHLLGRMRNKNPDL VDPPAFLKHLLTLTANGMNVNQ
Sbjct  241  EQLRTLMHGLNTARHVTTLLKGCEHLLGRMRNKNPDLTVDPPAFLKHLLTLTANGMNVNQ  300

Query  300  TLQLTQHIGGARLKNQLAFLNGLRPMLMQLPILLWKDMKSRQAALNNLLTLMAELTQQEQ  359
            TLQLTQHIGG +LK+QLAFLNGLRPML+QLPILLW+DMKSRQ+AL+NLL LMAELT QEQ
Sbjct  301  TLQLTQHIGGKQLKHQLAFLNGLRPMLLQLPILLWRDMKSRQTALSNLLILMAELTTQEQ  360

Query  360  KQLFEGLA  367
            KQL+ GL 
Sbjct  361  KQLYGGLV  368

3) Psyr_1216 in NC_007005


> AF232004|10|G9|11276508|AAG33876.1|Pseudomonas_syringae_pv._tomato_DC3000|+|11358..12464|hrpJ|-|HrpJ
Length=368

 Score =  469 bits (1768),  Expect = 1e-133, Method: Compositional matrix adjust.
 Identities = 288/369 (79%), Positives = 336/369 (92%), Gaps = 2/369 (0%)

Query  26   MKIVAPPTLpirpvapiraitpaaraipGGGLPDEKGTSSLQVSRFAAALVQHSRILRER  85
            MKIVAPP + I PVAP R +TPAA+++P ++L ++ GTS+ QVSRFAAAL+QH RIL +R
Sbjct  1    MKIVAPPIMRILPVAPTRVVTPAAQPLPNADL-HNSGTSPQQVSRFAAALIQHRRILIQR  59

Query  86   ELIASRNALQSRAVKLGELYQLLMSASDTGLDNAARLLRKKLLQDNDA-DLEQVLEFADG  144
            +LIAS NALQSRAVKLGELYQLLM+  DTGLDNAAR+LRKKL+QD+ + DL QVL+++DG
Sbjct  60   DLIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQDDSSSDLKQVLDYTDG  119

Query  145  DAAKAHVVLQAARKQAEDDGAEAEYVALTQTLKHLRRQFGPRTRAGINTARAFGRQNIDN  204
            DAAKAHVVLQAARKQAE DG  +E V LTQ LK+LRR++GPR+RAGIN+A+AF+R NIDN
Sbjct  120  DAAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKYGPRARAGINSAKAFARPNIDN  179

Query  205  KRRTALRNLYGVAVSGQPNVTGLIEALIGEQQEPGEFDLNLRDMRIAIADDLSAITPSAS  264
            KRR ALRNLY+VAVSGQPN+TGLIEAL+GEQQE+G+F+++LRDMR AIADDLSA+TPSAS
Sbjct  180  KRRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQFNIDLRDMRTAIADDLSAMTPSAS  239

Query  265  HEQLRTLMHGLTTARHVTTLLRGCEHLLGRMRKKNPKLTVDPPAFLKHMLTLTANGMNVN  324
            HEQLRTLMHGL TARHVTTLL+GCEHLLGRMR+KNP LTVDPPAFLKH+LTLTANGMNVN
Sbjct  240  HEQLRTLMHGLNTARHVTTLLKGCEHLLGRMRNKNPDLTVDPPAFLKHLLTLTANGMNVN  299

Query  325  QTLQLTQHIGGNRLEHQLAFLNGLRPMLMQLPILLWRDLKSRQAALNNLLTLMAELTQKE  384
            QTLQLTQHIGG++L HQLAFLNGLRPML+QLPILLWRD+KSRQ+AL+NLL LMAELT +E
Sbjct  300  QTLQLTQHIGGKQLKHQLAFLNGLRPMLLQLPILLWRDMKSRQTALSNLLILMAELTTQE  359

Query  385  QKQLYEGLA  393
            QKQLY GL 
Sbjct  360  QKQLYGGLV  368

4) Pmen_0038 in NC_009439


> AF232004|10|G9|11276508|AAG33876.1|Pseudomonas_syringae_pv._tomato_DC3000|+|11358..12464|hrpJ|-|HrpJ
Length=368

 Score =  231 bits (862),  Expect = 4e-62, Method: Compositional matrix adjust.
 Identities = 177/339 (53%), Positives = 251/339 (75%), Gaps = 14/339 (4%)

Query  47   KFAAALVQNSRELGRRDLFAKINALQSQASKQhelahlllatheRGMEESARALRKTLKN  106
            +FAAAL+Q+ R L +RDL+A  NALQS+A K  EL +LL++ ++ G++++AR LRK L +
Sbjct  44   RFAAALIQHRRILIQRDLIASNNALQSRAVKLGELYQLLMTDQDTGLDNAARVLRKKLMQ  103

Query  107  GQLLRMERGKLLKQIVDSMagdaakahallqaaarqakgEKSlneqqalnqqlqllrqqH  166
            +     + +  LKQ++D   GDAAKAH +LQAA +QA ++  + E+  L QQL+ LR++ 
Sbjct  104  D-----DSSSDLKQVLDYTDGDAAKAHVVLQAARKQAEADGEMGEHVVLTQQLKQLRRKY  158

Query  167  GRNPRPLFNTGNNTRILARGGEDPQRRDKLRSLYSVAVTEQLNVVGLIEALLNSTQDDGQ  226
            G  +R+     N+++ +AR   D +RR  LR+LYSVAV+ Q N+ GLIEALL   Q+ GQ
Sbjct  159  GPRARA---GINSAKAFARPNIDNKRRVALRNLYSVAVSGQPNITGLIEALLGEQQEAGQ  215

Query  227  KDGREGFEAVLREIRQVVARDMAELDGSASPQKLRTMMYAMSTAQYVATLFGGCEHLLGR  286
                  F+  LR++R  +A D++ +  SAS ++LRT+M +++TA+ V+TL+ GCEHLLGR
Sbjct  216  ------FNIDLRDMRTAIADDLSAMTPSASHEQLRTLMHGLNTARHVTTLLKGCEHLLGR  269

Query  287  MRNKNPAMQVETSAFLKNLLGLIGKGMALNDTLQLTQQIGGRQLKHQLAFLNGLRPMLHQ  346
            MRNKNP + V+ +AFLK+LL L ++GM +N+TLQLTQ+IGG+QLKHQLAFLNGLRPML Q
Sbjct  270  MRNKNPDLTVDPPAFLKHLLTLTANGMNVNQTLQLTQHIGGKQLKHQLAFLNGLRPMLLQ  329

Query  347  LPIMLWRDIKSRQNALGNLLSLMAALTSEEQKRLKEGIA  385
            LPI+LWRD+KSRQ AL+NLL LMA LT++EQK+L  G+ 
Sbjct  330  LPILLWRDMKSRQTALSNLLILMAELTTQEQKQLYGGLV  368