Gene Information

Name : entE (N175_19255)
Accession : YP_008490130.1
Strain :
Genome accession: NC_022225
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 4374 - 6014 bp
Length : 1641 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGAGTTACTCACTGTATAAGGGATATAAAATAATGATGAACGATGATTTTACACCTTGGCCTGAAAACATAAGTAAAAG
GTATAAAGAACTAGGCTATTGGCAAGACAAAACCTTATTTGATCACCTTCATGAAACTGCTATGTCTTCCCCAGATGCGC
CTGCCATTTGCTGTGGTGACATCACCTACAGCTACCAGGACTTCTTGGATCGTATCTATAAGCTATCTGCCGGGTTTATG
CAATTAGGTCTTAAGCCCGGGGATAATGTCGTATTGCAAATCAATAACACTGCTGAATTCTATTTTTCATTTTTCGCTTT
GACTATGAAAGGGATAACACCCGTTCTTGCTTTACCCGCCCATCGCTACTTGGAGTTAAGCTACTTTTGTAATCATGCTG
GCGCTAGAGCCTACATTTTTTCAGATCATGTATCTGGTTTAACCTCGCGTAACATGGCTAATCAATTGATTAAAGAATGC
AAAACTCTAGACTATGCTATTACAACAGGTGAAACAGGTCAGAGTAACCTTATTTCATTACAAAATTTATATATCACCCC
TTGTATGAAGCAATATACCACTGCAGATAAAGTGGCTTTTTTCCAGTTATCAGGAGGGACAACAGGCACTCCTAAGCTCA
TTCCCAGAACGCACAATGATTATGCTTATAGTATTGTGGGAAGTAATCGCATCTGTCAATTTAACGCCGAGACACGTTAT
CTTTGTACCCTTCCTGCTGCTCACAATTTCCCACTAAGCTCTCCGGGAGCTTTAGGAACCTTCTTTGCTGGAGGGTGTAT
CGTGCTCAGTTTAGATAGTTCCCCTGAGAATAATTTTTCATTAATTGAAAAACATAGAGTCAATACATGCGCACTCGTTC
CTCCCCTAGCCTTACTATGGATGCAATATGCACAAAACGCGCAACAAGATATTTCGAGCTTAGAGCTCATTCAAGTAGGG
GGAGCAAAATTTAGTGAAAATGCCGCAAATAAATTCCCGCTGATTTTGGACTGTACGTTACAACAGGTATTTGGTATGGC
TGAAGGTCTTGTGAATTACACACGGTTAGACGATCCGATAGATATCATCACGAAAACACAAGGTCGTCCTATTTCAGAAC
ATGATGAAATACTAATAGTGAGTGAAGAAGGCCTACCCGTAATGTTAGGAGAAGAAGGTCAGTTAATGACTAGAGGCCCA
TACACCATACGAGGTTACTACAAAGCGGCAGGACACAATCAACGTTCATTCGATAAAAACGGATTTTACGCGACGGGAGA
TCTTGTTCGGCAAACTCCTGAAGGTAACATCATTGTGACAGGAAGGAATAAAGACCAAATAAATAGAGGTGGGGAAAAAA
TTGCTACAGAGGAAATTGAAAATATACTACTTGAGCATAATGGCATTCATGACGTAGCACTAGTAGCAGTTCCTGACGAG
TTTTTGGGAGAAAAAAGCTGTGCGATAATCGTATGTAACCAAAATGTTAACCTTAAGCCAGTTTCAATAAAGAAATTTTT
ATATGAGCAAGGTATCGCAGAATATAAGATCCCAGATCACATTAATTTTCGCCAAAAATTACCTAAAACTGCTGTTGGAA
AAATAAACAAAAAGGCCTTGAGAGCCGAATATAGTACTTAA

Protein sequence : 
MSYSLYKGYKIMMNDDFTPWPENISKRYKELGYWQDKTLFDHLHETAMSSPDAPAICCGDITYSYQDFLDRIYKLSAGFM
QLGLKPGDNVVLQINNTAEFYFSFFALTMKGITPVLALPAHRYLELSYFCNHAGARAYIFSDHVSGLTSRNMANQLIKEC
KTLDYAITTGETGQSNLISLQNLYITPCMKQYTTADKVAFFQLSGGTTGTPKLIPRTHNDYAYSIVGSNRICQFNAETRY
LCTLPAAHNFPLSSPGALGTFFAGGCIVLSLDSSPENNFSLIEKHRVNTCALVPPLALLWMQYAQNAQQDISSLELIQVG
GAKFSENAANKFPLILDCTLQQVFGMAEGLVNYTRLDDPIDIITKTQGRPISEHDEILIVSEEGLPVMLGEEGQLMTRGP
YTIRGYYKAAGHNQRSFDKNGFYATGDLVRQTPEGNIIVTGRNKDQINRGGEKIATEEIENILLEHNGIHDVALVAVPDE
FLGEKSCAIIVCNQNVNLKPVSIKKFLYEQGIAEYKIPDHINFRQKLPKTAVGKINKKALRAEYST

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-90 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-91 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 9e-91 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-91 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-91 44
ybtE CAA21394.1 - Virulence HPI Protein 9e-91 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-89 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 7e-91 44
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008490130.1 enterobactin synthase subunit E VFG0932 Protein 1e-114 49
entE YP_008490130.1 enterobactin synthase subunit E VFG0168 Protein 1e-116 46
entE YP_008490130.1 enterobactin synthase subunit E VFG1817 Protein 1e-95 41