Gene Information

Name : entE (N175_10460)
Accession : YP_008488503.1
Strain :
Genome accession: NC_022223
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 2223258 - 2224889 bp
Length : 1632 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
GTGGTCATGAATAATCTTAACCCGACCGATTTTACCCCTTGGCCAAAGCAATTCAGCCAGCGCTATCGTGAACAAGGCTA
CTGGCAACAAAGAACCTTATTTGATTATTTCAGTGATTCCGTAAAAAAAAGCCCTGACGCCATCGCACTGATTGATGATT
TCGGCCAGTACAGCTATCAAAATTTATATCAGAAAATGATCAGCCTTGCGGCTGGCTTGAGCTCCCTAGGTTTGCAGCGG
GGTGATAATGTCGTACTACAAATGGCCAATCGTGCTGAATTCTATCTCTGCTTTTTTGCCTTGACCATGCGGGGCATTAA
ACCGGTTCTAGCTCTGCCAGCGCACCGCTATCTAGAGCTGAGCTATTTTTGCCAACATACTCACGCCAAAGCCTATATTT
TTTCAGACGATATCGTGGGCTTTGACGCGCAGCAAACCGCTCAGAAACTACGAAGTAGTTGCCCTTCTCTCTTGCATACC
ATTGTTTGCGGTCAATCCTCACACGAGCAAATTCAGGAGTTAGATACGCTTTACCACTGCGCGGGAGACGATCCTGTTAC
TGATTCGGTCTTTGCTGATCAAGTGGCTTTTTTCCAGCTTTCCGGCGGAACAACTGGCACTCCCAAATTGATCCCTCGTA
CGCACAATGACTACGCTTATAGCGTACTTGGTAGCATTCAAATTTGTCAGTTTGGTCCACAAACGAGGTATTTGTGTGTT
CTTCCCGTTGCGCATAACTTTCCACTCAGCTCGCCCGGCGCATTGGGCGTCTTTTACGCTGGCGGTTGTGTCGTGCTCGC
TTCCGATACCACACCGCACACCGCTTTCCGTTTAATTGAGAAGCACCACATTACCGTTGCAGCGCTAGTGCCGCCGTTAG
CGCTACTGTGGATGAAATACGCCGAGTCCGCTACAGAAAACATCGCTAGCCTCACTCTGGTACAAGTCGGCGGAGCGAAG
TTTAGTGAGGCCGCAGCAATGCGCTTACCGACGACTCTTCACTGTCAATTACAGCAAGTGTTTGGAATGGCTGAAGGGTT
GGTTAATTACACACGACTTGATGACCCACTCAGCGTGATTGTTACTACTCAAGGACGCCCAATTTCAACTGATGATGAAG
TGATGATAATCAACGATGAGGGTGAGCCCGCGGCGGTGGGTGAGGAAGGCTTACTCATGACCAGAGGCCCTTATACGATT
CGAGGTTATTACCGAGCTCCAGAACATAACCAACGCTCATTCACCGACCAAGGCTTCTATATCACAGGTGACAGAGTGCG
TAGGACGGCTGAAGGCAATATTATCGTCACGGGACGAGAGAAAGATCAGATCAATCGTGGTGGTGAAAAAATCGCAGCCG
AAGAGGTTGAAAACCTGCTACTTCAACATCAGGATGTTCATGATGTGGCTTTGGTTGCTATTGCTGATGAGTTTCTTGGT
GAACGCAGTTGCGCCATTGTCGTGCCAGATAACGGTAAAACGCTCAAACCCATTACTCTCAAACGATTTTTACACGCTCA
AGGATTAGCAGAATTTAAGATCCCTGATCACATCCATTTCGTCGATGAATTACCCAAAACCCCTGTGGGAAAAGTCGACA
AAAAATGGTTAAGAACGCAATACTCTCACTAA

Protein sequence : 
MVMNNLNPTDFTPWPKQFSQRYREQGYWQQRTLFDYFSDSVKKSPDAIALIDDFGQYSYQNLYQKMISLAAGLSSLGLQR
GDNVVLQMANRAEFYLCFFALTMRGIKPVLALPAHRYLELSYFCQHTHAKAYIFSDDIVGFDAQQTAQKLRSSCPSLLHT
IVCGQSSHEQIQELDTLYHCAGDDPVTDSVFADQVAFFQLSGGTTGTPKLIPRTHNDYAYSVLGSIQICQFGPQTRYLCV
LPVAHNFPLSSPGALGVFYAGGCVVLASDTTPHTAFRLIEKHHITVAALVPPLALLWMKYAESATENIASLTLVQVGGAK
FSEAAAMRLPTTLHCQLQQVFGMAEGLVNYTRLDDPLSVIVTTQGRPISTDDEVMIINDEGEPAAVGEEGLLMTRGPYTI
RGYYRAPEHNQRSFTDQGFYITGDRVRRTAEGNIIVTGREKDQINRGGEKIAAEEVENLLLQHQDVHDVALVAIADEFLG
ERSCAIVVPDNGKTLKPITLKRFLHAQGLAEFKIPDHIHFVDELPKTPVGKVDKKWLRTQYSH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-85 45
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-85 45
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 45
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-85 45
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 45
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-85 45
ybtE CAA21394.1 - Virulence HPI Protein 2e-85 45
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 8e-85 44
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-84 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008488503.1 enterobactin synthase subunit E VFG0932 Protein 5e-110 50
entE YP_008488503.1 enterobactin synthase subunit E VFG0168 Protein 9e-112 49
entE YP_008488503.1 enterobactin synthase subunit E VFG1817 Protein 1e-89 43