Gene Information

Name : pyk (BASU_2570)
Accession : YP_008421988.1
Strain : Bacillus amyloliquefaciens UCMB5113
Genome accession: NC_022081
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.1.40
Position : 2761352 - 2763109 bp
Length : 1758 bp
Strand : -
Note : Evidence 2a:Function of homologous gene experimentally demonstrated in an other organism; PubMedId:10559165, 10932722, 10966427, 11489127, 17022686; Product type e:enzyme

DNA sequence :
TTGAAAAAAACAAAAATCGTTTGTACAATCGGTCCGGCCAGTGAAAGTGTAGAAATGCTGACAAAATTGATGGAAGCGGG
TATGAACGTTGCTCGTCTGAATTTTTCACACGGTGATTTCGAAGAGCACGGCGCAAGAATCAAAAACATCAGAGAAGCAA
GCAAAAAGCTCGGCAAAAACGTGGGGATTCTCCTCGATACGAAAGGTCCGGAAATCCGCACGCACGATATGGAAAACGGC
GCGCTTGAGCTTCAGGCGGGAAATGAAATTATCGTTTCTACGAAACAGGTGCTGGGAACGCTTGAAAAATTCTCAGTTTC
TTATGAAGGCCTGGCAGACGACGTGTCAGCAGGGTCTATCATCCTGTTGGATGACGGTTTAATCGGATTGGAAGTGCTAG
AATCAAATCCGGAAAAACACGAAATCAAAACAAAAATCTTAAACAGCGGCACGCTGAAAAATAAAAAAGGCGTAAACGTT
CCCGGCGTCAGCGTCAACCTTCCCGGAATTACTGAAAAAGACGCCAAAGACATCGTATTCGGTATCGAGCAAGGCGTTGA
TTTCATCGCTGCGTCATTTGTACGCCGTTCTACGGATGTCCTTGAAATCCGCGAACTGCTTGAAGAGCATAACGCGTCTG
ACATTCAAATCATTCCTAAGATCGAGAACCAGGAAGGCGTGGACAACCTGGACGCGATCCTGGAAGTATCTGACGGCTTA
ATGGTAGCGCGCGGAGACCTCGGCGTAGAGATTCCGGCCGAAGAAGTGCCGCTCGTGCAAAAAGAAATGATCAAAAAATG
CAACGCGCTCGGCAAACCGGTTATTACTGCGACACAAATGCTTGACAGCATGCAGCGCAACCCTCGTCCGACACGCGCTG
AAGCAAGTGACGTTGCCAACGCCATTTTCGACGGCACAGACGCTATCATGCTTTCCGGTGAGACGGCTGCGGGACAATAT
CCTGTCGAGGCGGTTCAAACAATGTTTAACATCGCCACACGCACGGAAGAATCTCTGAACTACAAAGAAATTCTGTCTAA
ACGCAGAGATCAGGTCGGCATGACGATTACCGATGCGATCGGCCAATCAGTTGCGCACACGGCGATCAATCTGAGTGCGG
CAGCTATCGTTACCCCTACAGAAAGCGGCCATACGGCGCGTATGATCGCGAAATACCGTCCGCAGGCTCCGATTGTAGCC
GTTACGGTTAATGAGTCTGTATCACGCAAACTCGGTCTTGTATTCGGTGTATTCCCGGCAAGCGGCCAAAACGCGAATTC
AACCGATGAAATGCTTGAAGACGCGGTTCAAAAATCACTTGACAGCGGCATCGTGAAACGCGGTGATCTGATTGTCATTA
CAGCAGGTTCTGTCGGTGAATCAGGCACGACGAATCTGATGAAAGTCCACACTGTCGGCAGCGTTATCGCGAAAGGGCAA
GGCATCGGACGCAAATCTGCATTCGGTCCGGTCGTTGTTGCAAAAAATGCGAAAGAAGCTGAACAAAAAATGACAGACGG
CGCAGTATTGGTTGCTTCAAGCACTGACCGCGACATGATCGCTTCACTTGAAAAAGCATCAGCGCTCATCACTGAAGAAG
GCGGTCTGACAAGCCATGCAGCCGTTGTCGGTTTAAGCTTGGGCATTCCGGTTATCGTCGGTCTGGAGAACGCAACTTCA
GTTTTAACAGAAGGACAGGTTATCACAGTTGACGCTGCCCGCGGCGCTGTGTATGAAGGCCGCGCAAGCGTTCTTTAA

Protein sequence :
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHDMENG
ALELQAGNEIIVSTKQVLGTLEKFSVSYEGLADDVSAGSIILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNV
PGVSVNLPGITEKDAKDIVFGIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQY
PVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAIGQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNESVSRKLGLVFGVFPASGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSVIAKGQ
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
VLTEGQVITVDAARGAVYEGRASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-108 54
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-108 54
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-108 54
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-107 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_008421988.1 pyruvate kinase VFG0479 Protein 2e-108 54