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Name : lysS (M493_00645) Accession : REF_PRJNA208285:M493 Strain : Geobacillus sp. JF8 Genome accession: NC_022080 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : - COG ID : - EC number : 6.1.1.6 Position : 85503 - 86987 bp Length : 1485 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAGCCATGAAGAATTGAACGACCAACTGCGTGTCCGCAGGGAAAAATTGAACAAAATTGAAGAACTAGGCGTTGACCC GTTCGGCAAACGGTTCGAGCGCACGCATAAAGCGGAAGAACTGTTTGAACTGTACGGGGATTTGTCAAAAGAGGAACTCG AAGAGCAACAAATCGAAGTCGCCGTCGCCGGCCGCATCATGACGAAACGCGGCAAAGGAAAAGCGGGGTTTGCCCACATT CAGGACGTGACAGGGCAAATCCAAATTTACGTCCGTCAAGACGATGTCGGCGAACAGCAATACGAGCTGTTTAAAATCTC CGACCTTGGTGATATCGTCGGCGTGCGCGGCACGATGTTCAAAACAAAAGTTGGCGAGCTTTCTATCAAAGTATCGTCGT ATGAATTTTTAACAAAAGCACTGCGCCCGCTGCCGGAAAAATACCACGGTTTAAAGGACGTCGAACAGCGCTACCGCCAG CGCTATCTTGACTTAATTATGAACCCGCAAAGCAAGCAGACGTTCATTACCCGCAGTCTCATTATTCAATCGATGCGCCG TTATCTTGACAGCCATGGCTACTTGGAAGTCGAAACACCGATGATGCACGCCGTAGCAGGCGGCGCGGCGGCCCGCCCGT TCATTACGCATCATAATGCACTTGATATGACGCTGTACATGCGAATCGCCATTGAGCTGCATTTGAAACGGCTCATCGTC GGCGGTTTAGAAAAAGTGTATGAAATCGGACGTGTTTTCCGGAATGAAGGCATCTCCACCCGCCATAACCCGGAGTTTAC GATGCTCGAGTTGTATGAGGCGTATGCCGACTTCCGCGATATTATGCAATTAACGGAAAACTTGATCGCCCACATTGCCA CAGAGGTGCTTGGCACAACGAAAATCCAATATGGTGAACATATCGTCGATTTAACGCCTGAATGGCGGCGGTTCCACATG GTGGATGCGATCAAAGAATACGTCGGCGTCGATTTTTGGCAGCAAATGACCGATGAGGAAGCGAGGGCGTTGGCCAAAGA ACATGGCGTTGAAGTCGCCCCACACATGACGTTCGGCCATATCGTCAACGAGTTTTTTGAACAAAAAGTAGAGGATAAAC TAATCCAGCCGACGTTCATTTACGGCCACCCGGTCGAAATCTCGCCATTAGCCAAGAAAAACCCAGACGACCCGCGCTTT ACCGACCGGTTTGAGCTGTTTATCGTCGGGCGCGAACATGCGAACGCCTTTACGGAACTAAACGACCCGATCGATCAGCG TCAACGGTTTGAGGCGCAGCTGAAGGAGCGCGAACAAGGAAACGACGAAGCGCACGAAATGGACGAGGATTTCCTCGAAG CGCTTGAATACGGCATGCCGCCGACGGGCGGACTCGGGATCGGGGTCGACCGGTTAGTCATGCTTTTGACCAATTCACCG TCCATTCGCGATGTCCTTCTCTTCCCGCAAATGCGCCATAAATAA Protein sequence : MSHEELNDQLRVRREKLNKIEELGVDPFGKRFERTHKAEELFELYGDLSKEELEEQQIEVAVAGRIMTKRGKGKAGFAHI QDVTGQIQIYVRQDDVGEQQYELFKISDLGDIVGVRGTMFKTKVGELSIKVSSYEFLTKALRPLPEKYHGLKDVEQRYRQ RYLDLIMNPQSKQTFITRSLIIQSMRRYLDSHGYLEVETPMMHAVAGGAAARPFITHHNALDMTLYMRIAIELHLKRLIV GGLEKVYEIGRVFRNEGISTRHNPEFTMLELYEAYADFRDIMQLTENLIAHIATEVLGTTKIQYGEHIVDLTPEWRRFHM VDAIKEYVGVDFWQQMTDEEARALAKEHGVEVAPHMTFGHIVNEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNPDDPRF TDRFELFIVGREHANAFTELNDPIDQRQRFEAQLKEREQGNDEAHEMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSP SIRDVLLFPQMRHK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 6e-122 | 51 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 1e-92 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | REF_PRJNA208285:M493 | lysyl-tRNA synthetase | VFG1668 | Protein | 4e-122 | 51 |
