Gene Information

Name : M493_04450 (M493_04450)
Accession : REF_PRJNA208285:M493
Strain : Geobacillus sp. JF8
Genome accession: NC_022080
Putative virulence/resistance : Resistance
Product : cystathionine beta-lyase
Function : -
COG functional category : -
COG ID : -
EC number : 2.5.1.48
Position : 771538 - 772719 bp
Length : 1182 bp
Strand : +
Note : catalyzes the formation of cystathionine from L-cysteine and O-succinyl-L-homoserine; Derived by automated computational analysis using gene prediction method: GeneMarkS+.

DNA sequence :
ATGGAACGGGAATGGTCGTTTTCAACGAAACTGCTCCATAACGAATGGAAAGTCGACCGCGAAACGGGAGCGGTGAGTGT
GCCGATCCAGCACGCCTCCACGTTCCATCAGTTTGATTTCGACGCGTTCGGCAAATACGACTACAGCCGCTCCGGCAACC
CGACGCGCGAGGCGCTTGAGGAAGCGATCGCCGCCTTAGAAGGCGGCGTGCGCGGATTCGCCTTCGCCTCCGGCATGGCG
GCCATCTCGACCGCGTTTTTGCTTTTGTCCAAAGGCGACCATGTGGTTGTGACCGAAGACGTCTACGGCGGCACGTATCG
GATGGTCACGGAAGTGTTGAACCGGTTTGGCATCGAGCATACGTTTGTCGATATGACCGATTTGCATGCCGTGGCCGAAA
ACATCCGGCCGAACACGAAAGTCATTTATATGGAAACGCCGTCCAATCCGTTGTTAAAAGTGACCGACATTCAAGGCGTC
GTCAAGCTGGCGAAGGCGCACGGCTGTTTGACGTTTCTCGACAACACGTTTATGACGCCGGCGCTGCAACGGCCGCTTGA
CCTTGGCGTCGACGTCGTCCTCCATAGCGCGACGAAGTTTTTAGCCGGCCATAGCGATGTCGTCGCCGGGCTGGCGGCTG
TGAAGGATGAGGAATTAGGAAAACAGCTCTACAAGCTGCAAAACGCCTTTGGCGCCATCCTCGGCGTCCAAGACGCCTGG
CTCGTGCTGCGCGGCATCAAAACGCTCCACGTCCGGCTGAAGCAGTCATCCGAATCCGCCCTAGCCATCGCCGAGTATTT
GGCGCGCCACCCGAAAGTGGAAGAGGTGTACTATCCGGGGCTTTCGCACCACCCGGGCCATGCGGTTCAGCGCCAGCAGG
CGGCCGGGTTTGGCGCCGTCTTGTCGTTCCGACTCGCCGATGAAGACGCAGCCCGGACGTTCGTCCGAAACGTCCGCCTG
CCCGTGTTTGCTGTCAGCCTCGGAGCCGTCGAGTCGATTTTGTCATATCCGGCGAAAATGTCGCATGCGGCCATGCCGAA
AGACGAACGGCTCCGCCGCGGCATTACCGACGGCTTGCTGAGGCTGAGCGTCGGGCTGGAAGATGTGAACGATTTGATCG
CCGATTTCGAGCAGGCGCTGTCATATGTCAACGAACGGCCATCGGCCATCCCTGCGCGGTAA

Protein sequence :
MEREWSFSTKLLHNEWKVDRETGAVSVPIQHASTFHQFDFDAFGKYDYSRSGNPTREALEEAIAALEGGVRGFAFASGMA
AISTAFLLLSKGDHVVVTEDVYGGTYRMVTEVLNRFGIEHTFVDMTDLHAVAENIRPNTKVIYMETPSNPLLKVTDIQGV
VKLAKAHGCLTFLDNTFMTPALQRPLDLGVDVVLHSATKFLAGHSDVVAGLAAVKDEELGKQLYKLQNAFGAILGVQDAW
LVLRGIKTLHVRLKQSSESALAIAEYLARHPKVEEVYYPGLSHHPGHAVQRQQAAGFGAVLSFRLADEDAARTFVRNVRL
PVFAVSLGAVESILSYPAKMSHAAMPKDERLRRGITDGLLRLSVGLEDVNDLIADFEQALSYVNERPSAIPAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
metB YP_281510.1 cystathionine beta-lyase Not tested Not named Protein 7e-88 55
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-83 51
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 4e-82 50

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M493_04450 REF_PRJNA208285:M493 cystathionine beta-lyase BAC0207 Protein 1e-62 42