Gene Information

Name : M493_03855 (M493_03855)
Accession : REF_PRJNA208285:M493
Strain : Geobacillus sp. JF8
Genome accession: NC_022080
Putative virulence/resistance : Resistance
Product : cadmium transporter
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 663852 - 665978 bp
Length : 2127 bp
Strand : +
Note : Derived by automated computational analysis using gene prediction method: GeneMarkS+.

DNA sequence :
GTGCGGAGTGAACAGGCGCTGGATCAATGGGAAGCGAAAACGTACCGGGTGCAAGGATTGACGTGTCCAAACTGCGCGGC
CACGTTTGAGCGAAACGTGAAGTCCTTGCCCGGCGTGAAAGAGGCGAAAGTGAATTTTGGTGCCGCCAAGCTCACCGTTT
GGGGCGAGGCGACGATCGATGAGCTTGAACAAGCGGGAGCGTTTGAACAGTTGACCATTCGTGAAGAACGTGAGCGGACC
GTCCGGCCGGAGCCGTTTTGGAAGAGGAAGGAGAATCGGAACGTTTACGTCGCCGCCGCGTTGCTGGCCATCGGCTGGAC
GGCGTCGGCGGCGGGCAGTGGTCGGTTGGCGGTGGCTGCGTATGTCGCTGCGATTGTGATCGGCGGCTACTCGCTATTTC
GGGCTGGATTTCGTAACTTGATCCGCTGGCAGTTCGACATGAACACGTTAATGACGATTGCCGTTCTCGGCGCGGCGGCG
ATCGGGGAATGGCAGGAAGGGGCGGTCGTTGTCATTTTGTTTGCCATTAGCGAAGCGCTCGAGCGCTATTCGATGGAAAA
CGCGCGGTGGTCGATCGCGTCGTTAATGGAAATGGCGCCGGACGAGGCGACCATCCGGCGCGGCGCCGAGGAAGTGACCG
TTCCGGTCGAAGACGTTCGCGTCGGGGATGTGATGATTGTTAAGCCGGGTGGAAAAATTGCTATGGATGGCATCGTCATA
AGCGGCGCTTCGACTGTGAATGAAGCAGCGATTACCGGTGAAAGCTTGCCGGTAGAAAAAGCACCGGGGGCTGCGGTGTT
CGCCGGGACGCTTAACGGGGAAGGGTTCCTCGAAGTCGAAGTAACGAAGCGGGCGGATGAGACGACAGTGGCGAAAATGA
TCGATCTCGTCGAAGAGGCGCAAGCGGAGCGGGCCCCTTCGCAGGCGTTCGTCGACCGGTTTGCGCGCTATTATACGCCG
TTTATTATCGCGGTTGCGCTTCTGATCGCCGTCGTGCCGCCGCTCGTGATGGACGGGGCATGGCTTGATTGGGTGTATCG
CGGGCTCGCGGTGTTGGTGATCGGCTGTCCGTGTGCGCTCGTCATCTCCACCCCGGTGGCGATTGTCACCGCCATCGGAA
ACGCGGCCCGGCGCGGCGTGCTCATCAAAGGCGGCGCGCATCTTGAACAAATCGGCCGGCTGCGGGCGGTGGCCTTGGAT
AAAACGGGGACGTTGACGAAAGGGAAACCGGCCGTGACCGATGTCATCGTGTATGAAGGAACACGCGAGCAGCTGCTGGC
CATCGCCGCCGCCATCGAAAAACGGTCGCAGCACCCTTTGGCTTCAGCCATCGTCCGTCGGGCGGAAGAAGAGGGGACGC
CGTTTCTCGACGTGCCGGTTGGCGAGTTTCAGTCATTGACCGGCCAAGGGGTGAAGGCTGTTGTCGGAAGCGAAACATAC
TACATCGGCAGCCCCGCCTTGCTCACCGGCTTGATCGGGGAGTTGCCGGGTGAAGCGGAACAACAAATCGCTGTGTTCCG
CAGCGAAGGAAAAACGGTGATGGCCGTTGGCACCGCCAACCGGCTGTTGGGGCTTGTCGCTGCGGCCGATCCATTGCGGC
CGTCCGCTCCGGATACGATCGCGGCATTGCGGCGCCTTGGCGTGGCGGAAATCGCGATGGTGACAGGCGACCACAGGCAA
ACGGCACAGGCCATCGGCCGCCAAGCGGGCGTATCGGACGTTCGCGCCGGGCTGCTTCCCGAAGAGAAGCTGGCGGCGAT
CCGCGAGTTGAAACAACGTTGCGGCATAACCGCCATGGTCGGCGACGGGGTGAATGACGCTCCTGCCTTGGCCGCGGCCG
ATGTCGGCGTGGCGATGGGCGGGGCGGGGACCGACACCGCCTTGGAAACGGCTGATGTCGTGCTGATGGCCGATGACTTG
CGCCAGCTTCCGTACACGATCCACCTTGGGCGCCGAACGCTTGCCATCGTTAAACAAAACATCGCCATCGCCTTAGGGTT
CAAAGTGTTGGCACTCGTGGCTGCCGTCCCAGGCTGGCTCACCCTTTGGCTTGCGGTGTTCGCCGACATGGGGGCGACGC
TGCTCGTGACGCTCAACAGCATGCGCCTGTTGCGGGTGAAGGGTTAG

Protein sequence :
MRSEQALDQWEAKTYRVQGLTCPNCAATFERNVKSLPGVKEAKVNFGAAKLTVWGEATIDELEQAGAFEQLTIREERERT
VRPEPFWKRKENRNVYVAAALLAIGWTASAAGSGRLAVAAYVAAIVIGGYSLFRAGFRNLIRWQFDMNTLMTIAVLGAAA
IGEWQEGAVVVILFAISEALERYSMENARWSIASLMEMAPDEATIRRGAEEVTVPVEDVRVGDVMIVKPGGKIAMDGIVI
SGASTVNEAAITGESLPVEKAPGAAVFAGTLNGEGFLEVEVTKRADETTVAKMIDLVEEAQAERAPSQAFVDRFARYYTP
FIIAVALLIAVVPPLVMDGAWLDWVYRGLAVLVIGCPCALVISTPVAIVTAIGNAARRGVLIKGGAHLEQIGRLRAVALD
KTGTLTKGKPAVTDVIVYEGTREQLLAIAAAIEKRSQHPLASAIVRRAEEEGTPFLDVPVGEFQSLTGQGVKAVVGSETY
YIGSPALLTGLIGELPGEAEQQIAVFRSEGKTVMAVGTANRLLGLVAAADPLRPSAPDTIAALRRLGVAEIAMVTGDHRQ
TAQAIGRQAGVSDVRAGLLPEEKLAAIRELKQRCGITAMVGDGVNDAPALAAADVGVAMGGAGTDTALETADVVLMADDL
RQLPYTIHLGRRTLAIVKQNIAIALGFKVLALVAAVPGWLTLWLAVFADMGATLLVTLNSMRLLRVKG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 2e-176 56
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-175 54
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 2e-175 54

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M493_03855 REF_PRJNA208285:M493 cadmium transporter BAC0298 Protein 4e-103 51
M493_03855 REF_PRJNA208285:M493 cadmium transporter BAC0092 Protein 1e-95 44
M493_03855 REF_PRJNA208285:M493 cadmium transporter BAC0124 Protein 1e-86 44
M493_03855 REF_PRJNA208285:M493 cadmium transporter BAC0455 Protein 1e-83 42