Gene Information

Name : M493_16150 (M493_16150)
Accession : REF_PRJNA208285:M493
Strain : Geobacillus sp. JF8
Genome accession: NC_022080
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2986062 - 2987702 bp
Length : 1641 bp
Strand : -
Note : Derived by automated computational analysis using gene prediction method: GeneMarkS+.

DNA sequence :
ACGGTCGGGGGGACGTGCGTCAATATCGGCTGCGTGCCGTCAAAGACGTTGCTTCGGGCTGGGGAAATCAATGCGTTGGC
GCGCAATCACCCGTTTCTCGGCCTGCACACCTTTGCCGGTCCGGTGGACTTGGCGCCGTTAGTGAAGCAAAAAAATGAAT
TGGTCGGACAGCTTCGCCAAGCGAAGTATGCGGATTTAATCGCGGAGTACGGGTTCGATTTCATCCAAGGGGAAGCCCGA
TTCGTCGGACGTCAAACGATTGACGTCAACGGCCAAACGCTGTCGGCGAAACGGTTTTTGATTGCGACGGGCGCTTCCCC
GGCTGTGCCGGACATTCCGGGGCTCCATGATGTCGATTACTTGACAAGCACGACGCTGCTTGAGCTGAAAAAGGTGCCGA
AGCGGCTTGCTGTCATCGGCGCCGGTTACATCGGGATGGAGTTGGGGCAGCTGTTCCACCATCTTGGCTCCGAGGTGACA
TTGATGCAGCGGAGCCCGCGCCTCTTGAAAGCGTATGACCCGGAAATTTCCGAAGCGGTGGCAGAGGCGCTCGCGGAACA
AGGCATCCGCGTCATCACGGGCGCTTCGTTTGAACGGGTCGAACAAAACGGAAACACGAAAAACGTATATGTGAACGTAG
ACGGGCGCACCCGGGTCATCGAAGCGGACGAACTGCTTGTGGCGACGGGGAGAACGCCGAATACAGCCGCGTTGAACTTG
CCGGCAGCCGGTGTGGACGTCGGGGCGCGCGGGGAGATTTTGATTGACGAATACGCGCGGACGACGAACCCGTACATTTA
TGCGGCAGGCGATGTCACGCTTGGCCCGCAGTTTGTCTATGTCGCAGCCTATCAAGGCGCCGTTGCCGCAGCCAATGCCG
TCGGCGGATTGAACAAGCGCTGGGATACGGCCGTTGTTCCGGCGGTGACGTTCACCCATCCGGCCATCGCCACCGTCGGG
TTGACTGAGCAACAGGCGAAAGAAAACGGGTATGACGTGAAAACGTCTGTCTTGCCGCTTGAGGCGGTGCCGCGCGCGAT
CGTCAACCGGGATACGACAGGACTGTTTAAACTCGTGGCTGAGGCGCGCACGGGCAAGCTGTTAGGCGCCCATGTTGTGG
CAGACAATGCCGGTGATGTCATTTATGCCGCCACGTTGGCGATTCAGTTCGGATTAACGATTGACGATCTTCGCAGCACG
TTGGCTCCGTATTTAACGATGGCGGAGGGGCTGAAATTAGCGGCGCTGACGTTTGACCAAGATGTTTCGAAGTTATCGTG
CTGCGCGGGATGAGGCCTTCGAATGGGGCCGCCGCTTCGCGGACCGTTATGAAGGGGCTGAAACTCATTGCGGTTGCAAT
GAAGCGGAATAAGAAGCGATCCGTTTGGCCGGGCGCTTCCCGATTCATAACGAAATGTCGGCGCAACGTTCCTATTTCCT
TGACCGTGTACGATCGTGCACGGTGGATCCTTTTGGGTGAGGTGGTTATGTGAAACATTACCGCATCGGGGAGCTGGCAG
AAAAATGCCATGTCAATAAAGAAACCATTCGGTACTACGAACGGAAGGGGCTCATTCCCGAAACCGAGCGGACGGAAGGC
GGATACCGTCTGTATACGGAGGAAACCGTCAGGCGCATCCA

Protein sequence :
MKTYRMNVQGMTCTGCEQHVTAALEAIGAKQIDVSFRRGEAVFALPDGTEIGLAKQAVRNAHYEPGDIEEITVPATPSFD
GGGEYDYIVIGSGGAAFSSAIEAVKYGAKVAMVERGTVGGTCVNIGCVPSKTLLRAGEINALARNHPFLGLHTFAGPVDL
APLVKQKNELVGQLRQAKYADLIAEYGFDFIQGEARFVGRQTIDVNGQTLSAKRFLIATGASPAVPDIPGLHDVDYLTST
TLLELKKVPKRLAVIGAGYIGMELGQLFHHLGSEVTLMQRSPRLLKAYDPEISEAVAEALAEQGIRVITGASFERVEQNG
NTKNVYVNVDGRTRVIEADELLVATGRTPNTAALNLPAAGVDVGARGEILIDEYARTTNPYIYAAGDVTLGPQFVYVAAY
QGAVAAANAVGGLNKRWDTAVVPAVTFTHPAIATVGLTEQQAKENGYDVKTSVLPLEAVPRAIVNRDTTGLFKLVAEART
GKLLGAHVVADNAGDVIYAATLAIQFGLTIDDLRSTLAPYLTMAEGLKLAALTFDQDVSKLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 4e-180 67
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 3e-180 67
merA AGK07079.1 MerA Not tested SGI1 Protein 2e-84 44
merA ABQ57371.1 MerA Not tested SGI1 Protein 2e-84 44
merA AGK07021.1 MerA Not tested SGI1 Protein 2e-84 44
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 2e-82 43
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 7e-84 43
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 1e-81 43
merA AFG30120.1 MerA Not tested PAGI-2 Protein 1e-81 43
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 2e-81 43
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 1e-81 42
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 6e-82 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M493_16150 REF_PRJNA208285:M493 mercuric reductase BAC0224 Protein 0.0 70
M493_16150 REF_PRJNA208285:M493 mercuric reductase BAC0651 Protein 2e-180 67
M493_16150 REF_PRJNA208285:M493 mercuric reductase BAC0652 Protein 2e-80 42
M493_16150 REF_PRJNA208285:M493 mercuric reductase BAC0650 Protein 4e-76 42
M493_16150 REF_PRJNA208285:M493 mercuric reductase BAC0653 Protein 2e-79 41