Gene Information

Name : M493_14205 (M493_14205)
Accession : REF_PRJNA208285:M493
Strain : Geobacillus sp. JF8
Genome accession: NC_022080
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.1.40
Position : 2650952 - 2652715 bp
Length : 1764 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate; Derived by automated computational analysis using gene prediction method: GeneMarkS+.

DNA sequence :
TCCGTCGGCGCGAAAATTTTGCTCGATGACGGGCTCATCGGTCTCGAGGTCAATGCGGTCGACAAGCAAGCGGGAGAAAT
CGTTACAACCGTTTTAAACGGCGGCGTGCTGAAAAACAAAAAAGGGGTCAACGTCCCGGGCGTGCGCGTCAACTTGCCGG
GCATCACCGAGAAAGACCGCGCTGATATTTTGTTTGGCATTCGCCAAGGGATCGACTTTATCGCCGCTTCGTTCGTGCGC
CGGGCGTCCGATGTGCTGGAAATCCGTGAGCTGCTTGAGACGCACGATGCCCTTCATATCCAAATTATCGCCAAAATTGA
AAACGAAGAGGGCGTCGCCAACATTGACGAAATTTTAGAAGCCGCCGACGGCCTCATGGTGGCGCGCGGTGATTTGGGGG
TTGAAATTCCGGCTGAGGAAGTGCCGCTCATCCAAAAGCTGTTGATCAAAAAGTGCAATATGCTCGGCAAACCGGTTATT
ACGGCGACGCAAATGCTCGACTCGATGCAGCGCAATCCGCGGCCGACACGGGCGGAAGCAAGCGACGTTGCCAACGCCAT
TTTTGACGGCACCGATGCCGTCATGCTTTCCGGGGAAACGGCGGCTGGCCACTATCCGGTCGAAGCGGTGAAAACGATGC
ACCAAATCGCGCTCCGCACCGAACAGGCGCTTGAGCACCATGATATTTTAGCCCAACGCACAAAAGAAAGTGCGACGACC
ATCACCGATGCCATCGGGCAGTCGGTCGCCCATACGGCGCTCAACTTGGATGTGGCGGCGATCGTCACGCCGACCGTAAG
CGGAAAAACGCCGCAAATGGTGGCGAAATATCGCCCGCAAGCGCCGATTATTGCCGTCACCTCAAATGAAGCGGTATCGC
GCCGGCTGGCGCTTGTGTGGGGCGTGTATACGAAAGAGGCGCCGCATGTGAACACGACGGATGAAATGCTCGATGTCGCC
GTCGATGCAGCGGTGCGTTCCGGTTTGGTGAAGCACGGCGATTTGGTCGTCATCACCGCCGGCGTGCCGGTCGGCGAAAC
CGGTTCGACGAACTTAATGAAAGTACACGTCATCAGCGACCTTCTCGCCAAAGGGCAAGGCATCGGCCGCAAGTCGGCGT
TCGGCAAGGCCGTCATCGCGAAAACAGCGGAAGAAGCGCGGCAAAAAATGATAGACGGCGGCATTTTAGTCACCGTCAGC
ACCGATGCGGACATGATGCCGGCGATTGAAAAAGCGGCGGCGATCATTACGGAGGAAGGCGGGCTGACAAGTCATGCCGC
TGTGGTCGGCCTAAGCCTTGGGATTCCGGTCGTCGTCGGGGTCGGACAGGCGACAAGTTTGTTTAAAGACGGGCAAGAAA
TTACAGTCGATGGCGGGTTTGGCGCCGTCTACCGCGGCCATGCGAGCGTGTTGTAATACAGTTGTTGGTCGACAAAAACA
GCCTTGGACATGAAGCCAAGGCTGTTTTTATGAGGTGGGCTTGCCGATGATAAAGCGCCACATGTCGCGGAAGACGTTGG
CGCTGTGGAGCGTGCGGATGATGACGAGCGCGACCGGACCGGCGATGAGGCCGAGAAAACCGAGCAGCTTGAAGCCGACA
AACAAGGCGATCAGCGTCGCGAGCGGATCGAGGCCGATCGACGAGGAGAGCACCTTCGGCTCCATAATTTGCCGCTGGAC
GAGAACGACGATATACAAAACGCCAAGCCCGATCGCAAACGGGATATCCCCGCTGATGGCGGCGTAAATAATCCAAGGCA
CGAA

Protein sequence :
MKRKTKIVCTIGPASESTDKLVQLIEAGMNVARLNFSHGDHEEHGRRIANIREAARQTGKTVAILLDTKGPEIRTHNMEN
GAIELKEGTKLVISMSEVLGTPEKISVTYPGLVDDVSVGAKILLDDGLIGLEVNAVDKQAGEIVTTVLNGGVLKNKKGVN
VPGVRVNLPGITEKDRADILFGIRQGIDFIAASFVRRASDVLEIRELLETHDALHIQIIAKIENEEGVANIDEILEAADG
LMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGH
YPVEAVKTMHQIALRTEQALEHHDILAQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGKTPQMVAKYRPQAPII
AVTSNEAVSRRLALVWGVYTKEAPHVNTTDEMLDVAVDAAVRSGLVKHGDLVVITAGVPVGETGSTNLMKVHVISDLLAK
GQGIGRKSAFGKAVIAKTAEEARQKMIDGGILVTVSTDADMMPAIEKAAAIITEEGGLTSHAAVVGLSLGIPVVVGVGQA
TSLFKDGQEITVDGGFGAVYRGHASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 5e-112 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-112 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-112 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-112 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M493_14205 REF_PRJNA208285:M493 pyruvate kinase VFG0479 Protein 8e-113 53