Gene Information

Name : entE (CFSAN001921_24620)
Accession : YP_008258181.1
Strain :
Genome accession: NC_021815
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 168307 - 169884 bp
Length : 1578 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGAATTCTTCCTTTGAATCTCTGATTGAACAGTATCCCTTACCCATTGCCGAACAGTTGCGCCACTGGGCGGCCCGTTA
TGCCTCGCGAATTGCCGTCGTTGATGCAAAGGGGTCGTTAACCTACAGCGCGCTTGATGCACGGGTCGACGAACTTGCCG
CAGGTCTGTCATCACTGGGTTTACGTTCGGGGGAGCATGTGATTGTGCAGCTTCCCAACGACAACGCGTTTGTTACCCTG
CTGTTCGCCTTGTTAAGACTGGGCGTTATCCCCGTGCTGGCGATGCCCTCCCAACGGGCGCTGGATATCGACGCGCTGGT
TGAGCTGGCGCAACCCGTCGCTTACGTTATTCACGGGGAAAACCACGCAGAGCTGGCCCGACAGATGGCGCACAAACACG
CCTGCTTGCGTCATGTTCTGGTCGCTGGAGAGACCGTGAGCAACGATTTTACGCCGCTCTTCTCCCTTCACGGTGAGCAG
CAGGCGTGGCCGCAGCCTGATGTTTCCGCCACCGCGTTGCTGCTACTTTCTGGCGGCACAACCGGCACGCCCAAACTCAT
CCCTCGCCGACACGCCGACTATAGCTATAACTTCAGCGCTTCTGCTGAACTGTGCGGCATCAGTCAACAGAGCGTATATC
TCGCCGTCCTCCCGGTGGCGCATAACTTTCCGCTGGCCTGCCCCGGCATTCTGGGTACGCTTGCCTGCGGCGGAAAAGTG
GTGCTGACCGACAGCGCCAGCTGTGATGAGGTGATGCCGTTAATCGCGCGGGAAGGAGTGACTCATGTCGCCCTGGTTCC
GGCGCTGGCGCAATTATGGGTACAGGCCAGGGAGTGGGAAGACAGCGACCTCTCGTCGCTGCGCGTCATTCAGGCAGGCG
GCGCCCGGCTCGACCCGGCGCTTGCTGAGCAGGCTATCGCCACCTTTGACTGTACCCTGCAACAGGTCTTCGGTATGGCG
GAAGGCCTGCTCTGCTTTACCCGGCTGGACGATCCGCATACCACTATTCTCCACAGCCAGGGGCGCCCGTTGTCCCCTCT
GGATGAAATCCGCATCGTTGATCAAAACGAGAACGACGTCGCGCCGGGCGAAACAGGGCAATTGTTAACGCGCGGCCCTT
ATACCATTTCAGGTTATTACCGCGCCCCTGCCCACAACGCGCAGGCCTTTACCACGCAAGGGTTTTACCGCACAGGCGAC
AATGTCAGGCTGGATGAGGCGGGGAACCTGCACGTTGAGGGGCGCATAAAAGAGCAGATCAACCGCGCCGGAGAAAAAAT
AGCCGCGGCTGAAGTGGAGTCGGCACTGATGCGTTTAGCGGAAGTGCAAGATTGCGCGGTGGTCGCCGCGCCGGACACGC
TGCTTGGTGAGCGGATTTGCGCGTTTATCATCGCGCAGCAGGTGCCAACTGACTATCAGCAGTTGCGTCAACAACTGACC
CGTATGGGGCTCAGCGCGTGGAAAATTCCTGACCAAATCGAGTTTCTGGCCCACTGGCCACTCACCGCCGTCGGCAAGAT
AGACAAAAAACGCCTGACGGCTCTCGCCATCGACCGTTATCGCCATTCTGCCCAATAA

Protein sequence : 
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDARVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFALLRLGVIPVLAMPSQRALDIDALVELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSNDFTPLFSLHGEQ
QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAREGVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPALAEQAIATFDCTLQQVFGMA
EGLLCFTRLDDPHTTILHSQGRPLSPLDEIRIVDQNENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTTQGFYRTGD
NVRLDEAGNLHVEGRIKEQINRAGEKIAAAEVESALMRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLAHWPLTAVGKIDKKRLTALAIDRYRHSAQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 0.0 98
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 0.0 98
ybtE CAA21394.1 - Virulence HPI Protein 0.0 98
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 0.0 98
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 0.0 98
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 0.0 98
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 0.0 97
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 0.0 97
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 0.0 97
irp2E YP_005163485.1 siderophore biosynthetic protein Virulence Not named Protein 3e-75 42
irp2E YP_005134497.1 siderophore biosynthetic protein Virulence Not named Protein 2e-75 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008258181.1 enterobactin synthase subunit E VFG0168 Protein 3e-107 51
entE YP_008258181.1 enterobactin synthase subunit E VFG1817 Protein 2e-89 44
entE YP_008258181.1 enterobactin synthase subunit E VFG0932 Protein 2e-79 42