Gene Information

Name : entE (CFSAN002069_05865)
Accession : YP_008250013.1
Strain : Salmonella enterica CFSAN002069
Genome accession: NC_021812
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 1185986 - 1187596 bp
Length : 1611 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGCGTATACCTTTCACCCGTTGGCCGGATGAATTTGCCCGCCGTTATCGTGAAAAAGGCTACTGGCAAGACGTGCCGTT
GACCGATATTCTGACCCGCCACGCTGACAGCGATAAGACGGCGGTCATTGAAGGCGAGCGCGCATTCAGCTATCGCCAGC
TCAACCAGGCCGCGGATAATCTGGCCTGCTATTTACGCCGTCAGGGCATCAAACCTGGCGAAACCGCTCTGGTGCAACTG
GGGAATGTACCGGAACTGTATATCACTTTTTTCGCCCTGTTGAAGCTTGGCGTAGCGCCCGTTCTGGCGCTGTTTAGCCA
TCAACGTACCGAACTTAACGCCTATGCGATGCAGATCGCGCCGACGCTGGTGATTGCCGATCGCCAACATACGCTGTTCG
CCGGCGAGGACTTTCTCAACACGTTTGTGGCTGAACACCGCTCTGTGCGGGTGGTGTTATTGCGTAACGACGACGGCGAT
CACAGCCTGGACGCGGCGATGCGGCAGGCGGCGGAAGATTTCACTGCCACGCCATCACCTGCTGACGAAGTGGCCTACTT
TCAGCTTTCCGGCGGTACTACCGGCACGCCAAAGCTTATTCCCCGTACCCATAACGACTATTACTACAGCGTGCGCCGCA
GCAATGAGATTTGCGGTTTCAACGAGGAGACGCGTTTTCTGTGCGCGATTCCCGCCGCGCATAACTACGCCATGAGTTCG
CCGGGCGCGCTGGGCGTTTTTCTCGCCAAAGGAACGGTAGTGCTGGCGACCGATCCCAGCGCCACGCTCTGTTTCCCGCT
GATCGAAAAACACCAGATTAATGCCACGGCGCTGGTGCCGCCCGCGGTCAGTCTATGGCTACAGGCTATCCAGGAGTGGG
GCGGCAATGCGCCGCTGGCGTCATTAAGGTTATTGCAGGTCGGCGGCGCGCGGCTTTCTGCGACGCTGGCCGCCCGTATT
CCGGCTGAAATTGGTTGTCAGTTGCAGCAGGTCTTCGGCATGGCGGAAGGGTTAGTGAACTATACCCGGCTGGACGATAG
TCCGGAACGGATTATCAATACCCAGGGAAGACCCATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGCCGACGGGAATC
CACTGCCGCCGGGCGAGATTGGTCGTCTGATGACGCGCGGCCCCTATACTTTTCGCGGCTATTTCAACAGTCCGCAACAC
AATGCCAGCGCCTTTGACGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCCATTGATCAAGACGGCTACATCACCGT
TCACGGGCGTGAAAAAGATCAGATCAATCGGGGCGGCGAGAAGATAGCCGCCGAAGAGATAGAAAACCTGTTACTGCGCC
ACCCGGCGGTGATCCATGCGGCGCTGGTCAGCATGGAAGATGAACTGCTGGGGGAGAAAAGTTGCGCATATCTGGTGGTA
AAAGAGCCGCTGCGAGCGGTACAGGTACGCCGTTTCCTGCGAGAGCAGGGCGTGGCGGAATTTAAATTACCGGATCGCGT
GGAGTGCGTTGCGTCACTGCCGCTGACGCCGGTTGGTAAAGTCGATAAAAAACAATTACGCCAGCGGTTGGCGTCACGTT
CACCGCTCTGA

Protein sequence : 
MRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKTAVIEGERAFSYRQLNQAADNLACYLRRQGIKPGETALVQL
GNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDGD
HSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMSS
PGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQH
NASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLVV
KEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-88 42
ybtE CAA21394.1 - Virulence HPI Protein 2e-88 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-88 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-88 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-88 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-88 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-88 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-88 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-88 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008250013.1 enterobactin synthase subunit E VFG0932 Protein 0.0 87
entE YP_008250013.1 enterobactin synthase subunit E VFG0168 Protein 4e-115 46
entE YP_008250013.1 enterobactin synthase subunit E VFG1817 Protein 1e-89 42