Gene Information

Name : lysS (M495_20300)
Accession : YP_008231968.1
Strain : Serratia liquefaciens ATCC 27592
Genome accession: NC_021741
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : -
COG ID : -
EC number : 6.1.1.6
Position : 4245496 - 4247013 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAGCAACAACCACAAGGCGCCGATCAGGCGTTGGATCTCAATAACGAGTTGCAGTCACGCCGTGAGAAACTGGC
TGCGCTGCGCGAGAACGGCATTGCGTTCCCGAATGATTTTCGTCGTGACAACACGTCAGACAAGCTGCATGCAGCGTACG
GCGAAAAAGACAACGAAGAGCTGGAAGCGCTGGGCGTAGAAGTCACAGTAGCCGGCCGCATGATGACGCGCCGTATTATG
GGTAAAGCGTCATTTGTCACGCTGCAGGACGTTGGCGGTCGTATCCAGCTGTACGTTGCGCGCGACGATCTGGCGGAAGG
CGTTTACAACGAACAATTCAAGAAGTGGGACCTGGGCGATATCGTTGGCGCGCGCGGCAAGCTGTTCAAGACCAAGACCG
GCGAGCTGTCCATCCACTGCACCGAACTGCGTCTGCTGACCAAGGCCCTGCGCCCGCTGCCGGACAAGTTCCATGGTCTG
GCGGATCAGGAAACCCGTTACCGTCAGCGTTACCTGGATCTGATCGCCAACGACGAATCGCGCAAGACCTTCCAGGTGCG
CTCTCAGATCATGGCGGCAATGCGTAACTTCATGGTGGAGCGCGGCTTCATGGAAGTCGAAACTCCTATGATGCAGGTGA
TCCCTGGCGGTGCTTCCGCACGTCCGTTTGTGACACACCACAATGCGCTGGACATCGATATGTACCTGCGTATCGCGCCA
GAGCTGTACCTGAAGCGTCTGGTCGTCGGCGGTTTCGAGCGTGTGTTCGAGATCAACCGTAACTTCCGTAACGAAGGCGT
TTCTCCGCGTCATAACCCAGAGTTCACCATGATGGAACTCTACATGGCTTACGCGGATTACAAAGATCTGATCGAACTGA
CCGAATCCCTGTTCCGTACCCTGACCCAGAAAGTGCTGGGTAACACTCAGGTACAGTACGGTGATGAAGTGTTCGACTTC
GGTAAGCCGTTTGAAAAGCTGACCATGACCGAAGCGATCAAAAAATACCGTCCGGAAACCGATCTGGCCGATCTGGCTGA
CATGGGCAAAGCGGTTGCTATCGCCGAATCCATTGGCATCAAGGTTGAGAAGAGCTGGGGCCTGGGTCGCGTAGTGACCG
AGATCTTCGAAGAAGTGGCGGAAAGCCATCTGATTCAACCGACCTTCATTACTGAATACCCGGCGGAAGTATCCCCGCTG
GCACGTCGTAATGATAACAACCCGGAAATCACCGACCGCTTCGAGTTCTTTATCGGCGGCCGTGAAATCGGTAACGGCTT
CTCCGAGCTGAATGACGCAGAAGACCAGGCGCAGCGCTTCGCTGACCAGGCTAATGCGAAAGACGCCGGCGATGATGAAG
CCATGTTCTACGACGAAGACTACGTCACTGCGCTGGAGCACGGTTTACCGCCAACTGCAGGCCTGGGTATCGGTATCGAC
CGTATGGTAATGCTGTTCACTAACAGCCACACCATCCGTGACGTGATCCTGTTCCCGGCCATGCGCCCGCAGAAATAA

Protein sequence :
MSEQQPQGADQALDLNNELQSRREKLAALRENGIAFPNDFRRDNTSDKLHAAYGEKDNEELEALGVEVTVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVARDDLAEGVYNEQFKKWDLGDIVGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDESRKTFQVRSQIMAAMRNFMVERGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIELTESLFRTLTQKVLGNTQVQYGDEVFDF
GKPFEKLTMTEAIKKYRPETDLADLADMGKAVAIAESIGIKVEKSWGLGRVVTEIFEEVAESHLIQPTFITEYPAEVSPL
ARRNDNNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFADQANAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-171 68
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 6e-93 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_008231968.1 lysyl-tRNA synthetase VFG1668 Protein 2e-171 68