Gene Information

Name : entE (M495_17625)
Accession : YP_008231448.1
Strain : Serratia liquefaciens ATCC 27592
Genome accession: NC_021741
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 3677893 - 3679521 bp
Length : 1629 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGAGCATTGCTTTTACTCCCTGGCCTGAGGCACTAGCCCGTCGCTATCGCGAACGGGGATATTGGACCGACCGGCCATT
GACCGACATCATCACCCGCCAGGCTAAAAATGACGCCATCGCGCTGATCGACCCGCAACGCAGCCTGAGCTATCGCCAGC
TTAATCAACTCTCCGATCGGCTGGCTGCGGCCTTGCTGCGTCGGGGCATACACAGCGGTGATACCGCGCTGGTGCAGTTG
GGTAACGTGGTCGAGTTCTACGTGACTTTCTTTGCGCTGTTGAAAATTGGCGTGGTGCCGGTGAATGCGCTGTTTAGCCA
CCAACGCAATGAGCTGAACGCCTATGCGGCGCAGATCAAACCGGCGTTGCTGATTGCCGATCGTCAGCATGGCCTGTTCG
GTAACGACGAATTCCTGACGACGTTTCGCGCTGAACATCCTTCATTGCGCCTGGTCGCTTTGCGCAGTGAGGAACAGGGC
GAGCAGTCGTTGGCGAGTTGGCTGGAAGAGGATTGCGGCGATTTCGTGGCTGCGCCAACCGCGGCCGATCAGGTAGCGTT
CTTCCAACTCTCCGGTGGCAGTACCGGCACGCCGAAGCTGATCCCACGTACCCACAACGACTACTACTACAGCATTCGCC
GCAGCGTGGAGATTTGTCATTTTGATGACGAAACCCGTTATCTGTGCGCACTGCCGGTAGCACACAACTATCCGATGAGC
TCGCCTGGCGTATTGGGCGTGTTTTACGGTGGTGGGCTGGTGGTGTTCGCCAGCGATCCGGATGCCGGGCAATGCTTCGG
TTTGATCGACCAGCATCAGATCAACGTGACTGCGCTGGTGCCGCCGGCGGTAACGCTGTGGCTGCAGGCCATTGAGGAAT
GGGGCGGTTGCCAGCAGTTGGCCAGCCTGAAGCTGCTGCAGGTGGGCGGTGCCAAGCTGGGGGAAACCCTGGCCGCGCGC
ATTCCGGCCGAAATCGGCTGTCAGCTGCAGCAGGTGTTCGGCATGGCGGAAGGCCTGGTGAACTACACCCGGCTGGATGA
TGACGAGCAGCATATTTTGACTACTCAGGGCTGCCCGATGTCGCCGGATGATGAACTTTGGGTGGCCGATGACGACGGTA
CTCCGCTGCCTGTCGGCGAGACCGGGCGTCTGATGACCCGTGGCCCCTACACCTTCCGTGGTTATTACCAAAGCCCGGAA
CATAATGCCGCCGCCTTCGATAAAGACGGTTTCTACTGCTCCGGCGACTTGATTAGCCTGACTGAAGACGGCTACGTGAA
GGTTGAAGGGCGTCAGAAAGACCAAATCAACCGCGGTGGTGAGAAGATTGCCGCCGAGGAGATCGAAAACCTGCTGCTGC
GTCACCCGGAAGTGATCAATGCGGCGTTGGTTTCGATGCCGGACGAGTTGATGGGCGAAAAAAGCTGCGCCTACATTATC
GCCACCAGCCCATTGAAACCGGTCGTTTTGCGTCGCCATTTACGCGCCGAGGGCGTGGCCGAATTCAAATTACCCGACCG
CTTTATACAGGTTGAGACGCTGCCGCTCACCCCGGTTGGCAAAGTGGATAAAAAACTGTTGCGCCAACGCCTGGAGGCGC
AACAACTGACTCAGGTCCAGGGAGAATAA

Protein sequence : 
MSIAFTPWPEALARRYRERGYWTDRPLTDIITRQAKNDAIALIDPQRSLSYRQLNQLSDRLAAALLRRGIHSGDTALVQL
GNVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAAQIKPALLIADRQHGLFGNDEFLTTFRAEHPSLRLVALRSEEQG
EQSLASWLEEDCGDFVAAPTAADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDDETRYLCALPVAHNYPMS
SPGVLGVFYGGGLVVFASDPDAGQCFGLIDQHQINVTALVPPAVTLWLQAIEEWGGCQQLASLKLLQVGGAKLGETLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDDEQHILTTQGCPMSPDDELWVADDDGTPLPVGETGRLMTRGPYTFRGYYQSPE
HNAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYII
ATSPLKPVVLRRHLRAEGVAEFKLPDRFIQVETLPLTPVGKVDKKLLRQRLEAQQLTQVQGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-80 44
ybtE CAA21394.1 - Virulence HPI Protein 8e-80 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-80 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-80 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 8e-80 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-80 44
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-79 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 7e-80 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-80 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008231448.1 enterobactin synthase subunit E VFG0932 Protein 0.0 71
entE YP_008231448.1 enterobactin synthase subunit E VFG0168 Protein 2e-102 47