Gene Information

Name : lysS (J450_02020)
Accession : YP_008219466.1
Strain : Mannheimia haemolytica D171
Genome accession: NC_021738
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : -
COG ID : -
EC number : 6.1.1.6
Position : 430292 - 431794 bp
Length : 1503 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAAGTAGAAAATCAAGAATTAGATCTAAACGGTGAAATGTTAGCCCGCCGTGAAAAATTAAACAAAATCCGTGA
GCAGGGTAACGCATTTCCAAATACATTCCGTCGTGATGCGTTAGCCAAAGATCTGCACGCTCAATATGATGCAGTGGAAG
GTGAAACCTTAAAAGAACAGAATATTCAAGTGAAAGTTGCCGGTCGTATTATGTTAAAACGAGTAATGGGTAAGGCATCT
TTCTTTACCATTCAAGATGTGAGCGGTGCGATTCAGCTTTATGTAGCTCGTGATAACTTAAGTGACAGCGTTTACGAAGA
AAAAGTCAGCCTTTGGGATTTAGGCGACATTGTGGGCGTCGAAGGTGCGTTATTTAAAACCAAAACCGGTGAATTAACGG
TTCGTTGCTCTGAAGTTCAACTTTTAACCAAGTCTTTACGCCCGTTGCCGGATAAACATCACGGTTTAACGGATTTAGAA
ACTCGTTATCGTCAGCGTTATTTAGACTTGATTTCTAATGAAGAATCTCGCCGTACTTTTATGATCCGCTCGCAAGTAGT
TTCAGGCATTCGCAAATTCTTCTTAGAAAGAGACTTTATTGAAGTTGAAACGCCAATGTTGCAAGTGATCCCGGGCGGTG
CGGCAGCGAAACCGTTTATCACTCATCATAATGCGTTAGATGTGGATATGTATCTGCGTATTGCTCCAGAGCTTTACTTA
AAACGTTTAGTTGTGGGTGGTTTTGAGCGAGTATTTGAGCTTAACCGTAACTTCCGTAACGAAGGGGTTTCAGTACGCCA
CAATCCTGAATTTACAATGATTGAGTACTACCAAGCCTATGCGGATTATCACGATTTAATGGATAATACCGAAGAACTGT
TACGCCAATTAGCCTTAGATATTCTTGGCACAACTGACGTACCTTATGGTGATTATGTATTCGATTTCGGTAAACCGTTT
GAGCGTATTACGATGCACGATGCGATTGTGAAATATGGTAACGGCATTCAACGTGAAGATTTAGACAGCTTCGAGAAAAG
TGTGGAAATTGCGAAAGGCTTAGGCATTGAAATTCAAAAATCTTGGGGTTTAGGCTCGGTAGTCAATGCGATTTTTGAAG
AAGTGGCAGAACATCAATTAATTCAGCCAACTTTCTTGATGGCTCACCCGGCAGAAATTTCACCATTAGCTCGTCGTAAT
GATGAAAACCCAGAGGTAACTGATCGTTTTGAGTTATTTATCGGTGGTCGTGAAATTGGTAATGGTTTCTCAGAATTAAA
CGATGCAGAAGACCAAGCAGAGCGTTTTGATGCTCAAGTTGCGGCGAAAGATGCCGGTGATGATGAAGCAATGTTTAAAG
ATGAAGACTTTATCGTGGCATTGGAACACGGTCTGCCGCCAACAGCGGGTGAAGGTTTAGGAATTGACCGCTTAGCAATG
ATTTTCGCTAACGCCCCATCAATCCGTGATGTAATTTTATTCCCAGCGATGCGTCAGAAGTAA

Protein sequence :
MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKDLHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKAS
FFTIQDVSGAIQLYVARDNLSDSVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPF
ERITMHDAIVKYGNGIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRN
DENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFIVALEHGLPPTAGEGLGIDRLAM
IFANAPSIRDVILFPAMRQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-148 62
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-85 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_008219466.1 lysyl-tRNA synthetase VFG1668 Protein 6e-149 62