Gene Information

Name : entE (M621_18170)
Accession : YP_008160406.1
Strain : Serratia plymuthica S13
Genome accession: NC_021659
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 3815336 - 3816961 bp
Length : 1626 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGAGCATTGATTTTACTCCCTGGCCTGAGGCGTTGGCCCATCGTTATCGTGAACGCGGTTATTGGACCGACCGGCCATT
GACCGACATTATTGCCCGCCAGGCCAAAAATGACGCCGTGGCGCTGATCGACCCGCAGCGCAGCCTGAGCTATCGCCGGC
TCAATCAACTTTCTGACCGGCTGGCCGCCGCGCTGCAACGTCGGGGCTTGCACAGTGGCGATACCGCTCTGGTGCAGTTG
GGCAACGTGGTCGAGTTCTATGTGGCCTTCTTTGCGCTGTTGAAAATTGGCGTGGTGCCGGTGAACGCGCTGTTCAGCCA
TCAACGCAACGAACTCAAGGCTTATGCCGCACAAATCAAACCTGCGCTGCTGATTGCCGATCGCCAGCATGGCCTGTTCG
GCAACGACGAATTCCTCACGGCGTTTCGCGCCGAAAATCCTTCATTGCGCGTAGTGGCGCTGCGCAGCCAGGAGGAAGGC
GAGCAGTCGCTGGCCGCCTGGCTCGAAGAGGATGCCGAAGATTTTATCGCGACACCGACCCCGGCTGACCAGGTGGCGTT
CTTCCAGCTTTCCGGTGGCAGCACCGGCACGCCAAAGCTGATCCCGCGCACCCACAATGATTACTACTACAGCATCCGTC
GCAGCGTGGAAATTTGCCATTTCGATGCGCATACCCGCTACCTGTGCGCGCTGCCGGTGGCGCATAACTACCCAATGAGT
TCTCCGGGCGTGCTCGGCGTGTTTTATGGCGCCGGGCTGGTGGTGTTCGCCAGCGATCCGGATGCCGGGCAATGCTTCCG
TTTGATCGAGCAGCACCAGATTAACGTGACGGCGCTGGTGCCGCCGGCGGTGACGCTGTGGCTACAGGCGATTGAAGAGT
GGGGCGGCCAACCATTGGCCAGCCTTAAGCTGTTGCAGGTGGGGGGCGCCAAGCTGAGTGAAACCTTGGCCGCGCGTATT
CCGGCGGAAATCGGCTGCCAGTTGCAGCAGGTCTTCGGCATGGCGGAAGGTCTGGTGAATTACACCCGGTTGGACGATGA
CGAGCGGCATATTCTCACCACTCAGGGCTGCCCGATGTCGCCGGACGACGAACTGTGGGTGGCCGATGACGAGGGCAACC
CGCTGCCGATGGGGCAAACCGGGCGCCTGATGACCCGGGGCCCTTACACCTTCCGCGGTTATTACCAAAGCCCCGAACAT
AACGCCGCCGCCTTTGATAAAGACGGTTTTTACTGTTCCGGGGATCTGATCAGCCTGACTGAAGACGGCTATGTGAAGGT
GGAAGGGCGTCAGAAAGATCAGATCAACCGCGGTGGGGAGAAGATCGCCGCCGAGGAGATCGAAAACCTGCTGTTGCGCC
ACCCGGAGGTGATCAATGCGGCGCTGGTTTCCATGCCGGATGAGCTGATGGGCGAGAAGAGCTGCGCCTTTATTATCGCC
GCCAGCGCGTTGAAGCCGGTGGTGCTGCGCCGCCATTTGCGTGCAGAGGGCGTGGCCGAATTTAAATTACCTGACCGCTT
TATACAGGTCGATACGCTGCCGCTCACCCCGGTAGGAAAAGTGGATAAAAAACGGTTGCGTCAACGCCTGGAGACGCAAT
CACTGACTCAGGCCCAGGGAGAATAA

Protein sequence : 
MSIDFTPWPEALAHRYRERGYWTDRPLTDIIARQAKNDAVALIDPQRSLSYRRLNQLSDRLAAALQRRGLHSGDTALVQL
GNVVEFYVAFFALLKIGVVPVNALFSHQRNELKAYAAQIKPALLIADRQHGLFGNDEFLTAFRAENPSLRVVALRSQEEG
EQSLAAWLEEDAEDFIATPTPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDAHTRYLCALPVAHNYPMS
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGQPLASLKLLQVGGAKLSETLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDDERHILTTQGCPMSPDDELWVADDEGNPLPMGQTGRLMTRGPYTFRGYYQSPEH
NAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAFIIA
ASALKPVVLRRHLRAEGVAEFKLPDRFIQVDTLPLTPVGKVDKKRLRQRLETQSLTQAQGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-88 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-88 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-88 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-88 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-88 44
ybtE CAA21394.1 - Virulence HPI Protein 3e-88 44
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-88 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-88 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-88 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008160406.1 enterobactin synthase subunit E VFG0932 Protein 0.0 71
entE YP_008160406.1 enterobactin synthase subunit E VFG0168 Protein 3e-109 47