Gene Information

Name : entE (H650_21615)
Accession : YP_008110061.1
Strain : Enterobacter sp. R4-368
Genome accession: NC_021500
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 4379142 - 4380752 bp
Length : 1611 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate;

DNA sequence : 
ATGACCATTCCGTTTACCCGCTGGCCGCACGAGTTCGCCGAGCGTTACCGTGAAAAGGGCTACTGGCAGGATTTACCGCT
GACCGATATTTTGACCCGCCACGCGCGCAGTGATGCCACGGCGGTGATCGAGGGCGAACGCCGCTTGAGCTACCGCCAGC
TTAACGATGCCGCAGACAATCTGGCCTCGGCGTTACAGGCGCAGGGCATCAGGCGCGGGGAAACCGCGCTGGTGCAGCTG
GGCAACGTCAGCGAGTTCTACATTGTTTTCTTCGCGCTGCTGAAAATCGGCGTGGTGCCAGTCAACGCGCTGTTCAGCCA
TCAGCGCAGCGAATTGAACGCGTATGCCGCGCAAATCGCACCGGCGCTGCTGATTGCCGATCGCGCGCATACGCTATTTG
AGAATGACCATTTCCTCACCGCGTTTGTTGCACAGCACCCGTCGGTGCGCACTGTGCTGCTGCGCGGCGACAGCGGCGAG
CAGGCGCTGGAAGACGCCATTGCCCGCCCGGCAGATAATTTTATCGCCACGCCAACGCCCGCCGATGAAGTGGCGTTTTT
CCAGCTTTCTGGCGGCAGCACCGGCACGCCAAAACTTATCCCGCGCACACACAATGATTACTACTACAGCATTGTGCGCA
GTAACGAGATCTGCGGATTTAACGCGCAAACCCGTTACCTCTGCGCGCTGCCGGCGGCGCACAACTACCCGTTGAGTTCA
CCCGGCGCGCTTGGCGTCTTCCACGCCGGAGGCTGCGTGGTGCTGGCTGCCGATCCGAGCGCCACGCTCTGCTTCCCGCT
GATTGAAAAACACCAGATTAACGTTACCGCGCTGGTGCCGCCGGCGGTAAGCCTGTGGTTACAGGCGATCGCCGAGTGGG
GCAGCAATGCGCAACTCGCCTCGCTGCGGCTGTTGCAGGTCGGCGGGGCGCGTTTATCGGCAACGCTTGCGGCGCGTATT
CCGGCGGAGATCGGCTGCCAGTTGCAGCAGGTATTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTGGACGATCC
CTTTGACCGCATTATCAACACGCAAGGCCGCCCGATGTGCCCGGATGATGAAGTGTGGGTGGCGGATGAAAACGGCAATC
TGCTGCCGCCAGGCCACGTTGGCCGCCTGATGACGCGCGGCCCGTACACCTTTCGCGGCTACTTTAATAGCCCGGAACAT
AATGCCAGCGCCTTTAACGAACACGGTTTTTACTGCTCCGGCGATCTGATTTCGATCGACGAACAGGGCTATATCACCGT
GCAGGGACGGGAAAAAGATCAGATCAACCGTGGGGGCGAGAAGATTGCCGCCGAGGAGATCGAAAACCTGCTGCTGCGCC
ATGAGTCGGTGATCCACGCCGCGCTGGTCAGTATGGAAGACAGCCTGCTGGGTGAAAAAAGCTGCGCTTACCTGGTGGTG
AAAACGCCGCTGCGCGCGGTAGCTGTCCGCCGCTTTCTGCGAGAGCAGGGCGTTGCCGAGTTCAAACTGCCGGATCGTGT
GGAGTGCGTGGACGCGCTGCCGCTGACGCCGGTGGGTAAGGTCGATAAAAAACAGTTACGTCAGTGGTTAGCGCAACGCG
CACCGGCGTAA

Protein sequence : 
MTIPFTRWPHEFAERYREKGYWQDLPLTDILTRHARSDATAVIEGERRLSYRQLNDAADNLASALQAQGIRRGETALVQL
GNVSEFYIVFFALLKIGVVPVNALFSHQRSELNAYAAQIAPALLIADRAHTLFENDHFLTAFVAQHPSVRTVLLRGDSGE
QALEDAIARPADNFIATPTPADEVAFFQLSGGSTGTPKLIPRTHNDYYYSIVRSNEICGFNAQTRYLCALPAAHNYPLSS
PGALGVFHAGGCVVLAADPSATLCFPLIEKHQINVTALVPPAVSLWLQAIAEWGSNAQLASLRLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDPFDRIINTQGRPMCPDDEVWVADENGNLLPPGHVGRLMTRGPYTFRGYFNSPEH
NASAFNEHGFYCSGDLISIDEQGYITVQGREKDQINRGGEKIAAEEIENLLLRHESVIHAALVSMEDSLLGEKSCAYLVV
KTPLRAVAVRRFLREQGVAEFKLPDRVECVDALPLTPVGKVDKKQLRQWLAQRAPA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 4e-88 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-88 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-88 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-88 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-88 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-88 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 5e-88 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-88 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 4e-88 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_008110061.1 enterobactin synthase subunit E VFG0932 Protein 0.0 82
entE YP_008110061.1 enterobactin synthase subunit E VFG0168 Protein 3e-106 46
entE YP_008110061.1 enterobactin synthase subunit E VFG1817 Protein 7e-89 41