Gene Information

Name : lysS (H650_10770)
Accession : YP_008107971.1
Strain : Enterobacter sp. R4-368
Genome accession: NC_021500
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : -
COG ID : -
EC number : 6.1.1.6
Position : 2101159 - 2102676 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAACAAGCACAAGGCGCTGACGCGGCAGTCGATCTTAATAATGAACTGAAAACTCGCCGCGAGAAGCTGGC
GCAACTGCGTGAGCAGGGCGTGGCGTTCCCGAACGATTTCCGTCGCGATCAGACCTCTGACAAGCTACATGCGGAATTCG
ATGCCAAAGAGAACGAAGAGCTGGAAGCGCTGGGCATTGAAGTCGCCGTTGCGGGCCGTATGATGACCCGCCGCATCATG
GGTAAAGCCTCTTTCGTAACCTTGCAGGACATGGGGGGCCGCATTCAGCTGTATGTCTCTCGTGACGATCTGGCGGAAGG
TGTCTACAACGAGCAGTTCAAAAAATGGGATCTCGGCGATATCCTCGGCGCGCGCGGCAAGCTGTTCAAGACCAAGACCG
GTGAACTCTCTATCCATTGCACCGAGCTGCGCCTGCTGACTAAAGCGCTGCGTCCGTTGCCGGACAAATTCCACGGTTTG
CAGGATCAGGAAGCGCGCTATCGTCAGCGTTACCTGGATCTCATCTCGAATGATGAATCCCGCAACACTTTCAAAATTCG
CTCGCAGATCCTGGCCGGTATCCGCCAGTTCATGGTAGGCCGCGACTTTATGGAAGTGGAAACGCCGATGATGCAGGTGA
TCCCGGGCGGTGCGTCTGCGCGTCCGTTTGTCACCCACCATAATGCGCTGGATCTCGACATGTACCTGCGTATCGCGCCG
GAACTGTACCTTAAACGTCTGGTGGTTGGCGGTTTCGAGCGCGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTAT
CTCCGTTCGCCATAACCCTGAGTTCACCATGATGGAACTCTATATGGCGTACGCGGATTATAAAGATCTGATCGAGCTGA
CCGAATCTCTGTTCCGTACGCTTGCGCAGAACATCCTCGGCAAAACCGAAGTGCCGTACGGCGACGAAGTGTTTGATTTC
GGCAAGCCGTTCGAAAAACTGACCATGCGCGAAGCGATCAAGAAATACCGTCCGGAAACCAACATGGCGGATCTGGATAA
TTTCGACAGCGCGAAAGCAATTGCTGACAGCATCGGTATCAAGGTTGAGAAGAGCTGGGGTCTGGGCCGTATCGTCACGG
AGATCTTCGAAGAAGTGGCCGAAGCGCACTTGATCCAGCCGACCTTCATTACCGAATACCCGGCGGAAGTTTCGCCGCTG
GCGCGTCGTAACGATCAGAACCCGGAAATCACCGACCGCTTTGAGTTCTTCATTGGCGGACGTGAAATCGGTAACGGCTT
TAGCGAGCTGAACGACGCGGAAGATCAGGCGCAGCGCTTCCAGGATCAGGTCGATGCGAAAGCAGCGGGCGACGACGAAG
CAATGTTCTTCGACGAAGACTACGTGACCGCGCTGGAACACGGCTTACCGCCGACTGCCGGCCTGGGTATCGGTATCGAC
CGTATGGTGATGCTGTTCACCAACAGCCATACCATTCGCGATGTGATCCTGTTCCCGGCGATGCGTCCGGTGAAATAA

Protein sequence :
MSEQQAQGADAAVDLNNELKTRREKLAQLREQGVAFPNDFRRDQTSDKLHAEFDAKENEELEALGIEVAVAGRMMTRRIM
GKASFVTLQDMGGRIQLYVSRDDLAEGVYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
QDQEARYRQRYLDLISNDESRNTFKIRSQILAGIRQFMVGRDFMEVETPMMQVIPGGASARPFVTHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQNILGKTEVPYGDEVFDF
GKPFEKLTMREAIKKYRPETNMADLDNFDSAKAIADSIGIKVEKSWGLGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPL
ARRNDQNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFQDQVDAKAAGDDEAMFFDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-170 69
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-93 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_008107971.1 lysyl-tRNA synthetase VFG1668 Protein 8e-171 69