Gene Information

Name : SFUL_394 (SFUL_394)
Accession : YP_007929263.1
Strain : Streptomyces fulvissimus DSM 40593
Genome accession: NC_021177
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 457231 - 458874 bp
Length : 1644 bp
Strand : +
Note : Activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate; pathway:Siderophore biosynthesis; bacillibactin biosynthesis; UniProt-pubmed:21317323; UniProt-pubmed:8921902; UniProt-pub

DNA sequence :
GTGAGTACGCAAGCGGCGCCGACCTGGCCCGCCGAGTTCGCCGCGAAGTACCGGGCGGCCGGCTGGTGGCGCGGGGAGAC
CTTCGGCGAGATGCTGCGGCAGCGCGCCGAGGAGCACCCGGACCGGATCGCGATCGTCGACCCGGCGGCCGGGAGCCGGT
GGACCTACGCCGATCTCGACCGGCGCGCCGACCGGCTGGCCGCGGGCTTCCTCGCCCGCGGGATCGCCAAGGGCGACAAC
GTGGTGGTCCAACTCCCCAACATCGCCGAGTTCTTCGAGGTGATCTTCGCGCTGTTCCGGATCGGCGCGCTGCCCGTCTT
CGCGCTGCCCGCCCACCGCGAGACCGAGATCGCCTACTTCTGCGAGTTCACCGAGGCGGTCGCCTACGTCATCGCGGCCG
AGCACGGCGGCTACGACTACCGGGAGCTTGCGGCGAAGACCCGCGCCCACGTACCCACGCTGAAAAACGTGTTCGTCGCC
CAGGGGGACCCCGGCGACTTCGAGGCGCTGGCCGAGGTGGCCGAGGAGCCGGTCGGCTACGGCGGGCCGCGCCCCGACGA
CCTGGCCTTCCTCCAGCTCTCCGGCGGCAGCACCGGCGTACCGAAACTGATCCCGCGCACCCACGACGACTACATCTACT
CGCTGTGGGGCTCCAACGAGATCTGCGGGGTCGACGAGAACAGCGTCTACCTGGTCGCGCTGCCCGCCGCCCACAACTTC
CCTCTGTCGTCGCCCGGTTCGCTCGGCACGCTGTACGCGGGTGGTCGTGTGGTGCTGTGCCCGCAGCCCTCGCCCGAGGT
GGCCTTCCCGCTGATCGAGCGTGAGGGCGTGACCATCACCGGCCTGGTGCCGCCACTGGCCCTCCTCTGGACCGAGGCCG
CACCCGGCAGCGCGCACGACCTCAGCAGCCTGGACGTGCTGCTCGTGGGCGGCGCCAAGTTCAGCGAGGAGGCGGCACGC
AGGGTGCGTCCCGCGCTCGGCTGCACGCTCCAGCAGGTCTTCGGGATGGCGGAGGGGCTGGTCAACTACACGCGCCTGGA
CGACCCGGTGGAGACCATCGTCACCACTCAGGGCCGGCCCATCTCCCCGGACGACGAGATCCTGGTCGTCGACGACGAGG
ACCGGGAGGTGGAGCCCGGCGGGACGGGCCACCTGCTGACCCGGGGCCCGTACACGATCCGCGGCTACTGGAACGCCCCC
GAGCACAACGCCCGTTCCTTCACGGAGGACGGCTTCTACCGCACCGGCGATGTCGTCCGGGTCACGGAGAGCGGACACAT
CGTCGTGGAGGGGCGCGCCAAGGACCAGATCAACCGGGGCGGCGAGAAGGTCGCCGCCGAGGAGGTGGAGAACCACATCC
TCGCCCACCCTGCCGTGCACGACGCGAACGTGGTGGCCGAGCCCGACCCGTATCTGGGTGAGCGCGTCTGCGCGTACGTG
ATCCTGCGCTCGGGCGCCGGTCCGCTGAAGGCGGTGGCGATCAAACGGTTCGTACGGGAACGGGGTCTGGCCGCCTACAA
GGTGCCGGACCGGGTCGAGTTCGTGGACGCGTTCCCGCAGACCGGGGTCGGCAAGGTCTCCAAGAAGGACCTGCGCAACG
CCGCCGCCCGCACCGCCGCACCCGCGCCTGCTCCCGCCCTCTGA

Protein sequence :
MSTQAAPTWPAEFAAKYRAAGWWRGETFGEMLRQRAEEHPDRIAIVDPAAGSRWTYADLDRRADRLAAGFLARGIAKGDN
VVVQLPNIAEFFEVIFALFRIGALPVFALPAHRETEIAYFCEFTEAVAYVIAAEHGGYDYRELAAKTRAHVPTLKNVFVA
QGDPGDFEALAEVAEEPVGYGGPRPDDLAFLQLSGGSTGVPKLIPRTHDDYIYSLWGSNEICGVDENSVYLVALPAAHNF
PLSSPGSLGTLYAGGRVVLCPQPSPEVAFPLIEREGVTITGLVPPLALLWTEAAPGSAHDLSSLDVLLVGGAKFSEEAAR
RVRPALGCTLQQVFGMAEGLVNYTRLDDPVETIVTTQGRPISPDDEILVVDDEDREVEPGGTGHLLTRGPYTIRGYWNAP
EHNARSFTEDGFYRTGDVVRVTESGHIVVEGRAKDQINRGGEKVAAEEVENHILAHPAVHDANVVAEPDPYLGERVCAYV
ILRSGAGPLKAVAIKRFVRERGLAAYKVPDRVEFVDAFPQTGVGKVSKKDLRNAAARTAAPAPAPAL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-104 49
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-104 49
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-103 49
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-103 49
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-103 49
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-103 49
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-103 49
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-103 49
ybtE CAA21394.1 - Virulence HPI Protein 2e-103 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SFUL_394 YP_007929263.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 6e-129 55
SFUL_394 YP_007929263.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 3e-103 47
SFUL_394 YP_007929263.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 3e-106 45