Gene Information

Name : CLS_04660 (CLS_04660)
Accession : YP_007848286.1
Strain : Clostridium saccharolyticum K10
Genome accession: NC_021047
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : -
COG ID : -
EC number : 6.3.2.4
Position : 464954 - 466117 bp
Length : 1164 bp
Strand : -
Note : -

DNA sequence :
ATGAATCAATTTGATAACGAAGTAAGCAGCACTTTGAAAGAAATTGCGGAGCGCGTTGAAACACCGCCATACTTGAAAGA
CAGAATTGATAAAGAACTTTTGCGGCGCTGCCCAGAATATCGGAAGAAAATTGCCGTTATTTTCGGCGGTTGTTCCCCGG
AGTACAGCGTATCGCTGCAATCCGCATATGCGGTAATCAGCCATATGGATACTGAAGTATATACCCCTGTTCTGATCGGC
ATTTCTCAATCTGGCGATTGGTTTCTGTATGAGGGAGATATTGAAAAAATTGTTTCGGATACATGGTGTGGCGGGAAGGA
TTGTACGCCGGTTGTGGTATCACCGAACCGCACTTCGCATAATCTTTTGCTTTTGGAAAACGGCAAAGTGAAGAAACTGC
ATATAGATGCGGCCTTTCCGGTGCTGCACGGCAGAAACGGCGAAGATGGAACGATTCAGGGGCTTTTTGAAATGGCGGGG
ATTCCCGTTGTGGGATGTGGCGTCTTGTCCTCGGCCTTGTGTATGGATAAGGATCGGGCGCACAAGTTAGTACATACCGC
TGGAATTGCCGTTCCTTCCTCCTTTGTACTGGGGAAAAGTGCTTCGGTTGCGACAGCTTTGCAGCAAGCCGAAAAAATTG
GGTATCCGCTATTTGTAAAACCTGTAAAGGCGGGTTCTTCTTTTGGCATTACAAAGGTTTCTTCTTCTAATGAGCTTCCA
GCTGCCTTAGAGCTGGCCTTTGAATATGACAAACGGGTAATCGTAGAAGAATGTATTTCTGGTTTTGAAGTGGGCTGCGC
CATATTAGGCGATGAGGATTTAACCGTAGGTGAAGTGGATGAAATTGAACTGTCCGGCGGATTCTTTAACTTTACAGAAA
AATACACCTTAAAGACCTCTGCAATCCATGTTCCAGCAAGAATCCCGCAGAATACCGCAGAACAAATTAAGCAAGCCGCC
AAAACCATTTATCGGACGCTGGACTGCCAAGGATTTGCGAGGGTCGATCTGTTCCTGACTCCTTCCGGTGAAATTGTGTT
TAACGAAGTCAATACCATTCCCGGATTTACCACTCATAGCCGCTATCCGAATATGATGAAAGCAGCGGGTTTTACCTTTG
AGCAGATCGTGTCTGCGCTGATCGAACAGGCGGTGAGAGAATGA

Protein sequence :
MNQFDNEVSSTLKEIAERVETPPYLKDRIDKELLRRCPEYRKKIAVIFGGCSPEYSVSLQSAYAVISHMDTEVYTPVLIG
ISQSGDWFLYEGDIEKIVSDTWCGGKDCTPVVVSPNRTSHNLLLLENGKVKKLHIDAAFPVLHGRNGEDGTIQGLFEMAG
IPVVGCGVLSSALCMDKDRAHKLVHTAGIAVPSSFVLGKSASVATALQQAEKIGYPLFVKPVKAGSSFGITKVSSSNELP
AALELAFEYDKRVIVEECISGFEVGCAILGDEDLTVGEVDEIELSGGFFNFTEKYTLKTSAIHVPARIPQNTAEQIKQAA
KTIYRTLDCQGFARVDLFLTPSGEIVFNEVNTIPGFTTHSRYPNMMKAAGFTFEQIVSALIEQAVRE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 6e-71 47
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 1e-69 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 4e-72 49
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 8e-70 48
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 1e-71 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 2e-69 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 1e-71 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 5e-70 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 5e-70 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 5e-70 47
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase AF162694.gene.p01 Protein 8e-71 46
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase NC_005054.2598280.p0 Protein 2e-67 44
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase NC_014475.1.orf3.gen Protein 2e-67 44
CLS_04660 YP_007848286.1 D-alanine--D-alanine ligase L29638.gene.p01 Protein 2e-65 44