Gene Information

Name : pyk (Clst_2229)
Accession : YP_007680272.1
Strain : Clostridium stercorarium DSM 8532
Genome accession: NC_020887
Putative virulence/resistance : Virulence
Product : Pyk
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.1.40
Position : 2514838 - 2516601 bp
Length : 1764 bp
Strand : +
Note : pyruvate kinase; COG:0469 Pyruvate kinase; KEGG_module M00002:Glycolysis; KEGG_module M00001:Glycolysis; KEGG_module M00050:Guanine ribonucleotide biosynthesis IMP => GDP; KEGG_module M00049:Adenine ribonucleotide biosynthesis; KO:K00873 pyruvate kinase

DNA sequence :
ATGCGCAGAACTAAAATTATTTGTACCCTTGGTCCCGCATGCGAAAGTGAAGAAATCTTACGTGAGCTTGTTCTTGCGGG
CATGGATGTAGCCAGGATGAATTTTTCCCATGGCTCATATGAGGAACACAAACGCAGGATTGACATGCTGAAAAAAATCA
GGGAAGAAACAGGACGTCCAGTGGCGTTGCTGCTTGACACAAAAGGGCCTGAAATTCGCACGGGTAAGTTTAAAAACGGC
TCTGCCACCCTTTCCGAAGGGGATAGCTTTGTATTGACCCATGATGATATCCTTGGTGACGAGCATAAGGTATCGGTATC
ATACAAAGGATTGTACAAGGATGTTAAAAAAGGTGACCGAATCCTTATAGATGACGGGCTGATAGAACTGGAAGTAACAG
CCATTACAGGGAAGGACATTCACTGTGTTGTTTTAAACGGCGGCGTATTGGGCAATAACAAGGGAATCAATGTCCCGGGA
GTGGATATTAACCTTCCGTCCGTTACCCAGCAGGATATAGAGGACATCAAGTTTGGTATTGAAAATGACGTTGATTTTAT
TGCTGCTTCTTTTGTACGCAAAGCCGATGATGTAATTGAAATCAGAAAAATCCTGGATAAATACAACGGTCAGGATATTA
AAATCATATCCAAAATTGAAAACCGCCAGGGAGTAAACAATATCGACGAAATCCTTCTTGCTTCGGACGGAATTATGGTT
GCCCGCGGCGATTTGGGCGTTGAAATCCCCGTGGAAGAAGTTCCCGTTGTCCAGAAAATGCTTATTGAGAAATGTTTCCG
CAGCGGTAAACCTGTTATTACCGCCACTCAAATGCTTGACTCGATGATACGCAACCCAAGACCCACCCGTGCGGAAGCCA
GTGACGTAGCCAATGCCATTTACGATGGTACAAGCGCAATTATGCTTTCGGGAGAAACCGCATCAGGAAAATACCCGGTT
GAAGCTCTTAAAACGATGTCGAAAATAGCCGAAAAAGCAGAAAGTTCAATAGACTACTGGAAGCGTTTCATGAACACGCA
GCATGAAATGCTCCCTACGATTACCAACGCAATAAGCCATGCCACATGCACAACCGCGATGGATCTGAAAGCATCGGCAA
TTATTACGGTAACAAAATCAGGACATACCGCGCGCATGATTTCACGTTTCAGGCCCGAATGTCCCATTATTGCCACTACC
GTCAGCCCAAAGGTTCAACGCCAGCTGTCTCTGTGCTGGGGCGTGGTTCCGTTCCTGGTCAGCGAAGCGAAATCCACCGA
TGAGATGTTTGATACAGGCGTACAGAAAGCTTTGGAATCAGGTCTTGTAAAACACGGCGACCTGACCGTAATAACTGCCG
GTGTGCCCATAGGAGTAAGCGGTACGACTAACATTCTGAAAGTACAGCTTGTAGGCAAAGTACTTGTGAAAGGAACGGGT
ATTGGGACAGGCGTGATTACCGGCGAGTTATGCGTTGCCAAAACTGCCGATGAACTGAAAAGGAAATACGCAAAAGGCAA
CATTATTGTAGTGCCGTATACCACAAATGAAATGCTTCCGATGATAAAAAGCGCTTCAGCGCTAATTGTTGAGGAAAGCG
GCACAAACAATCACGCCGCTATCGTCGGTCTGGCACTGGACATACCGGTTATTGTAGGTGCACAGAATGCCACAAGCCTG
TTGAAAAGCGGATGTGTAGTTACCGTAGACGCAGAACGCGGAATTGTTCACAACGAAAACTTAATGCCATGCAGTCTGGA
ATAA

Protein sequence :
MRRTKIICTLGPACESEEILRELVLAGMDVARMNFSHGSYEEHKRRIDMLKKIREETGRPVALLLDTKGPEIRTGKFKNG
SATLSEGDSFVLTHDDILGDEHKVSVSYKGLYKDVKKGDRILIDDGLIELEVTAITGKDIHCVVLNGGVLGNNKGINVPG
VDINLPSVTQQDIEDIKFGIENDVDFIAASFVRKADDVIEIRKILDKYNGQDIKIISKIENRQGVNNIDEILLASDGIMV
ARGDLGVEIPVEEVPVVQKMLIEKCFRSGKPVITATQMLDSMIRNPRPTRAEASDVANAIYDGTSAIMLSGETASGKYPV
EALKTMSKIAEKAESSIDYWKRFMNTQHEMLPTITNAISHATCTTAMDLKASAIITVTKSGHTARMISRFRPECPIIATT
VSPKVQRQLSLCWGVVPFLVSEAKSTDEMFDTGVQKALESGLVKHGDLTVITAGVPIGVSGTTNILKVQLVGKVLVKGTG
IGTGVITGELCVAKTADELKRKYAKGNIIVVPYTTNEMLPMIKSASALIVEESGTNNHAAIVGLALDIPVIVGAQNATSL
LKSGCVVTVDAERGIVHNENLMPCSLE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-100 52
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-98 52
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-99 52
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-99 52

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_007680272.1 Pyk VFG0479 Protein 6e-100 52