Gene Information

Name : I653_15405 (I653_15405)
Accession : YP_007663831.1
Strain : subtilis subtilis BAB-1
Genome accession: NC_020832
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 3064002 - 3065621 bp
Length : 1620 bp
Strand : -
Note : COG1021 Peptide arylation enzymes

DNA sequence : 
ATGCTGAAAGGATTTACGCCTTGGCCTGATGAGTTAGCTGAGACCTATCGAAAGAACGGCTGCTGGGCCGGTGAAACGTT
TGGAGATTTGTTAAGGGACCGAGCAGCCAAATATGGTGATCGCATTGCCATTACATGCGGAAACACCCATTGGAGCTATC
GAGAGCTTGACACAAGGGCTGATCGCCTGGCTGCCGGATTTCAAAAGCTCGGCATTCAACAGATGGATCGTGTTGTGGTG
CAGCTGCCAAATATCAAAGAGTTTTTTGAAGTCATTTTCGCGCTTTTCCGTCTGGGGGCGCTTCCAGTCTTCGCACTGCC
TTCTCATCGAAGCAGTGAAATTACATATTTTTGCGAGTTTGCTGAAGCGGCTGCATATATCATTCCCGACGCATATTCCG
GTTTTGACTACCGTTCGCTTGCCAGACAGGTTCAAAGCAAGCTGCCGACTTTGAAAAACATCATTGTCGCAGGTGAGGCA
GAGGAATTTTTGCCGCTCGAGGATTTGCATACAGAACCTGTAAAACTGCCTGAAGTCAAATCTTGGGATGTGGCGTTCCT
CCAGCTTTCTGGCGGGAGCACCGGGCTATCGAAACTGATTCCGAGGACCCACGATGACTACATATACAGCCTGAAGCGGA
GTGTGGAGGTCTGCTGGCTCGACCACAGCACGGTGTACTTGGCCGCGCTGCCGATGGCACACAATTATCCGCTGAGCTCA
CCTGGCGTTCTCGGCGTATTATACGCTGGAGGAAGAGTGGTATTATCTCCTTCTCCAAGTCCAGATGACGCATTTCCTTT
GATTGCACGCGAAAAGGTCACGATAACCGCTCTTGTTCCGCCTCTTGCGATGGTATGGATGGATGCGGCATCCTCACGCC
GTGACGATTTATCCAGCCTTCAAGTGTTGCAGGTCGGCGGTGCCAAGTTTAGTGCTGAAGCCGCGCGCAGGGTAAAAGCT
GTTTTCGGCTGCACGCTGCAGCAGGTGTTCGGAATGGCAGAGGGTCTCGTCAATTATACGAGATTGGATGATCCTGAGGA
GATCATTGTCAACACCCAAGGAAGACCGATGTCCCCATATGATGAAGTGCGTGTTTGGGATGATCATGATCGCGACGTAA
AACCTGGTGAAACAGGCCATCTGCTGACGCGGGGACCGTATACAATTCGAGGTTACTATAAGGCAGAAGAGCATAACGCC
GCTTCATTTACTGAGGACGGTTTTTACCGTACGGGTGATATCGTCAGGCTGACACGAGACGGCTATATTGTCGTTGAAGG
CCGGGCGAAGGATCAAATTAACCGTGGAGGAGAAAAGGTTGCGGCTGAAGAGGTAGAAAATCATCTGCTGGCGCATCCAG
CTGTCCATGATGCGGCAATGGTCTCCATGCCTGACCAGTTTCTTGGTGAGAGATCTTGTGTGTTCATTATTCCCAGGGAT
GAAGCCCCAAAAGCCACAGAGCTCAAAGCATTTTTGAGAGAGCGCGGACTGGCGGCATATAAAATCCCTGATCGAGTTGA
ATTTGTCGAATCCTTTCCGCAGACAGGGGTAGGAAAGGTAAGCAAAAAGGCTCTTCGTGAAGCTATTTCCGAGAAACTTC
TTGCAGGGTCAAAAAAATAA

Protein sequence : 
MLKGFTPWPDELAETYRKNGCWAGETFGDLLRDRAAKYGDRIAITCGNTHWSYRELDTRADRLAAGFQKLGIQQMDRVVV
QLPNIKEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAAAYIIPDAYSGFDYRSLARQVQSKLPTLKNIIVAGEA
EEFLPLEDLHTEPVKLPEVKSWDVAFLQLSGGSTGLSKLIPRTHDDYIYSLKRSVEVCWLDHSTVYLAALPMAHNYPLSS
PGVLGVLYAGGRVVLSPSPSPDDAFPLIAREKVTITALVPPLAMVWMDAASSRRDDLSSLQVLQVGGAKFSAEAARRVKA
VFGCTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGRPMSPYDEVRVWDDHDRDVKPGETGHLLTRGPYTIRGYYKAEEHNA
ASFTEDGFYRTGDIVRLTRDGYIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVHDAAMVSMPDQFLGERSCVFIIPRD
EAPKATELKAFLRERGLAAYKIPDRVEFVESFPQTGVGKVSKKALREAISEKLLAGSKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-116 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-116 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-116 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-116 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-116 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-116 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-116 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-116 50
ybtE CAA21394.1 - Virulence HPI Protein 6e-116 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
I653_15405 YP_007663831.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG0168 Protein 8e-135 55
I653_15405 YP_007663831.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG0932 Protein 2e-109 48
I653_15405 YP_007663831.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG1817 Protein 3e-110 45