Gene Information

Name : H681_00040 (H681_00040)
Accession : YP_007655430.1
Strain : Pseudomonas denitrificans ATCC 13867
Genome accession: NC_020829
Putative virulence/resistance : Virulence
Product : N-6 DNA methylase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 11946 - 14330 bp
Length : 2385 bp
Strand : -
Note : COG0286 Type I restriction-modification system methyltransferase subunit

DNA sequence :
ATGAATTCCCCCAAGCTCCAAGACCAAAGCCAGATCAAGTGGATTTCCGACTTCATCTGGAACATCGCCGATGACCGCCT
GCGCGATGTGTATGTACGCGGCAAGTACCGCGACGTGATCCTGCCCTTCACCGTACTGCGACGGCTCGATGCAGTGCTAG
AAGCTACTAAGGACGCGGTGCTGGAGCGCAAGAAACTACTTGATGCCCACAACGTGGCAGAGCAGGACGGTGCACTACGT
GATGCTGCCAAGCAGGCGTTCTACAACGTTTCGGAATTCACCCTAGCCAAGCTGAAGGCTAGCGCGGCAGGCCAGCGCCT
GCGCGATGACTTCATTGCATATTTAGACGGCTTCTCGCCCAACGTGCAGGAGATCCTCACCAAGTTCAACTTCCGCAACC
AGATTCAGAAGCTAGTGGATTCCCACGTCCTCGGCTACCTGATCGACGACTTCCTCGACCCGGAGATCAATCTCGCGCCG
CTGCCGGTCAAGGATGCAGACGGGCGCATCAAGCTGCCTGCGCTGGACAACCACGGCATGGGCACCGTGTTCGAGGAGCT
GATCCGCCGCTTCAACGAAGAAAACAACGAAGAGGCGGGCGAACACTTCACGCCACGCGACGTGGTTCAACTCATGGCTA
AGCTGTTGTTCCTGCCCGTGGCTGACTGGATTGAATCCAGCACCTACTCGCTATACGACGGCAGCTGCGGCACAGGCGGG
ATGCTGACTGTTGCCGAAGAAGTCTTGCACGAACTGGCGGACGAACACGACAAGGAGGTGTCCATTCACCTGTTCGGCCA
GGAGATCAGCGACGAAACCTACGCCATCTGCAAGGCCGACTTGCTCCTGAAAGGCGAGGGCGCAGAGGCCGAGAACATAG
TTGGCGGTGCAGACAAATCGACCCTGTCCGCCGATCAGTTCCGCAGCCGCGAGTTCGATTTCATGATCTCCAACCCTCCC
TATGGCAAGAGTTGGAAAACCGATCTGGAGCGCATGGGCGGCAAGAAGGAATTCAACGATCCTCGCTTTATCGTCAGCCA
CGCGGGCGAGTCCGAGTTCAAGCTCATCACCCGCTCCAGCGACGGGCAGCTCATGTTCCTGGTGAACAAGCTGCAAAAGA
TGAAGCACAACACGCCGCTGGGCAGCCGCATCGCGCTGGTGCATAACGGCTCGGCGCTGTTTACCGGCGACGCAGGCCAA
GGTGAGAGCAACATACGCCGCTGGGTACTAGAGAACGACTGGCTGGAGGCCATCATCGCCCTACCGCTCAACATCTTCTA
CAACACCGGCATTGCCACCTATATATGGGTGCTGGCCAACAAGAAGGCCCAAGCGCGCCGTGGCAAAGTGCAGTTAATAG
ATGCATCGCAGTGGTTTCAGCCGCTGCGCCGTAACCTCGGCAAGAAGAACTGCGAACTGAGTGATTCTGACGTTGCCTGC
ATCCTCGATCTCTACCTCGGTGAAGCACAGGAAACCGCCCAGTCCAAATGGTTCGATACCCAGGACTTCGGCTACTGGAA
GATCACCGTCGAGCGCCCGCTACGCCTGAAAAGCCAACTCTCCGACGAACGCATTGAGCCCCTGCGTTTTGCCTCCAGCG
ACGAGGCACTGCGCGCCGAGATCTACGCCACCCACGGCGAAGCGCTCTACAGCGAGTTCGCCAAGCGCAAAACGGCCATC
GAGGCCTGGCTAAAAGGTGAGGATGAGAACGAAGACGAGGACAGTGAAGACGGCGACAGCGACGATGACAGTGAGACCCC
CGCAACCCGCAAGGCTGTGCCCGCCAAGCGCCGCAAGAAACTGCTCGACGCATCGACTTGGCAGCGTGACAAGCGGTTGC
TGGAGGTGGCGCAACGGGCTCGGAAGGCGCTGGGCAGCGCCGTGTTCGACGACCACAATGAGTTCCGCACCTGCTTCGAT
GCTGCACTCAAAGCGCAGGGCGACAAGCTCAGCGCGTCAGAGAAGAAGGCCATCTACAAGGCTGTCAGCTGGCGAGACGA
AACGGCTCCGCCCGTTATTGCAAAACGCACAAAACTCAAAGCCTCTGACCCCTTTGTGGGGAATTACGATGGTCGTTATC
TGTTAGATGAACACAACCACCGCGAAGTCGTGGAATATGAATCCGACGCGGATCTGCGCGACACCGAGCAGGTGCCGTTA
AAAGAGCCAGGCGGTATCGACGCCTTCTTTGCCCGCGAAGTGCGGCCGCATACACCAGATGCCTGGATCGCCACGGACAA
AACCCAGATCGGCTATGAAATTTCGTTCGCCCGCTACTTCTACAAGCCCACGCCACTGCGCACTCTGGCGGAAATCCGCG
CTGACATACTCGCGCTGGAGCAGCAGAGCGAAGGTTTGCTGCACAAGATTGTGGGGGGGGTGTGA

Protein sequence :
MNSPKLQDQSQIKWISDFIWNIADDRLRDVYVRGKYRDVILPFTVLRRLDAVLEATKDAVLERKKLLDAHNVAEQDGALR
DAAKQAFYNVSEFTLAKLKASAAGQRLRDDFIAYLDGFSPNVQEILTKFNFRNQIQKLVDSHVLGYLIDDFLDPEINLAP
LPVKDADGRIKLPALDNHGMGTVFEELIRRFNEENNEEAGEHFTPRDVVQLMAKLLFLPVADWIESSTYSLYDGSCGTGG
MLTVAEEVLHELADEHDKEVSIHLFGQEISDETYAICKADLLLKGEGAEAENIVGGADKSTLSADQFRSREFDFMISNPP
YGKSWKTDLERMGGKKEFNDPRFIVSHAGESEFKLITRSSDGQLMFLVNKLQKMKHNTPLGSRIALVHNGSALFTGDAGQ
GESNIRRWVLENDWLEAIIALPLNIFYNTGIATYIWVLANKKAQARRGKVQLIDASQWFQPLRRNLGKKNCELSDSDVAC
ILDLYLGEAQETAQSKWFDTQDFGYWKITVERPLRLKSQLSDERIEPLRFASSDEALRAEIYATHGEALYSEFAKRKTAI
EAWLKGEDENEDEDSEDGDSDDDSETPATRKAVPAKRRKKLLDASTWQRDKRLLEVAQRARKALGSAVFDDHNEFRTCFD
AALKAQGDKLSASEKKAIYKAVSWRDETAPPVIAKRTKLKASDPFVGNYDGRYLLDEHNHREVVEYESDADLRDTEQVPL
KEPGGIDAFFAREVRPHTPDAWIATDKTQIGYEISFARYFYKPTPLRTLAEIRADILALEQQSEGLLHKIVGGV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC0395_A1367 YP_001217310.1 DNA methylase HsdM Not tested VPI-2 Protein 4e-156 47
VC1769 NP_231404.1 DNA methylase HsdM Not tested VPI-2 Protein 4e-156 47
VPI2_0017c ACA01834.1 DNA methylase HsdM Not tested VPI-2 Protein 1e-155 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
H681_00040 YP_007655430.1 N-6 DNA methylase VFG1102 Protein 2e-156 47