Gene Information

Name : pyk (BSU6051_29180)
Accession : YP_007534905.1
Strain : Bacillus subtilis 6051-HGW
Genome accession: NC_020507
Putative virulence/resistance : Virulence
Product : pyruvate kinase Pyk
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.1.40
Position : 2984771 - 2986528 bp
Length : 1758 bp
Strand : -
Note : Evidence 1a: Function experimentally demonstrated in the studied strain; PubMedId: 10559165, 10932722, 10966427, 11489127, 17022686; Product type e: enzyme

DNA sequence :
ATGAGAAAAACTAAAATTGTTTGTACCATCGGTCCGGCAAGTGAAAGTATTGAAATGCTTACGAAATTAATGGAGTCAGG
AATGAACGTGGCTCGATTAAACTTTTCTCACGGAGATTTTGAGGAGCACGGTGCAAGAATTAAAAATATCCGCGAAGCAA
GTAAAAAACTTGGCAAGAACGTTGGAATTCTGCTTGATACAAAAGGTCCTGAAATCCGCACACATACAATGGAAAACGGC
GGTATTGAGCTTGAAACAGGCAAAGAGCTCATTATTTCAATGGACGAGGTTGTAGGAACAACAGATAAAATTTCAGTGAC
ATATGAAGGTTTAGTCCATGACGTTGAACAAGGTTCAACGATTCTGTTAGATGACGGCCTTATCGGTCTTGAGGTACTTG
ATGTAGATGCCGCTAAACGCGAAATCAAAACAAAAGTATTAAACAACGGAACACTCAAAAATAAAAAAGGTGTTAACGTA
CCGGGCGTAAGTGTCAATCTTCCGGGGATTACTGAAAAGGATGCGCGAGACATCGTTTTCGGTATTGAGCAAGGAGTAGA
CTTCATCGCACCATCTTTCATTCGACGTTCTACGGATGTGCTCGAAATCCGTGAGCTTCTTGAAGAGCACAACGCTCAGG
ATATTCAAATCATCCCTAAAATCGAAAACCAAGAGGGCGTTGACAACATCGATGCGATTCTCGAAGTGTCTGACGGCTTA
ATGGTTGCACGCGGAGACTTAGGTGTGGAAATTCCAGCTGAAGAAGTGCCGCTTGTGCAAAAAGAACTGATCAAAAAATG
CAACGCGCTGGGCAAACCTGTTATTACAGCGACACAAATGCTTGACAGCATGCAGCGCAACCCGCGTCCGACTCGTGCGG
AAGCAAGTGACGTTGCAAACGCGATCTTCGACGGAACAGATGCGATCATGCTTTCTGGTGAAACTGCTGCCGGAAGTTAC
CCGGTTGAAGCAGTTCAAACAATGCATAACATCGCGTCCCGTTCTGAAGAAGCATTAAATTATAAAGAAATTCTCTCAAA
ACGCAGAGACCAAGTGGGCATGACAATTACAGACGCAATTGGACAATCTGTCGCACATACGGCGATTAACCTGAATGCTG
CTGCGATCGTAACGCCGACAGAAAGCGGCCATACAGCACGTATGATTGCAAAATACCGTCCGCAGGCTCCGATTGTTGCG
GTTACTGTAAATGACTCTATTTCCAGAAAGCTTGCCCTCGTATCTGGCGTATTCGCGGAAAGCGGCCAAAATGCGAGCTC
AACAGATGAGATGCTTGAGGATGCTGTCCAAAAATCATTGAACAGCGGAATTGTAAAACACGGCGATCTTATCGTTATTA
CAGCTGGCACTGTCGGTGAGTCCGGCACTACGAACTTAATGAAGGTTCATACTGTCGGCGATATCATCGCTAAAGGCCAA
GGCATTGGACGCAAATCAGCTTACGGTCCGGTTGTCGTTGCACAAAATGCAAAAGAAGCTGAGCAAAAAATGACTGACGG
TGCGGTACTTGTTACCAAAAGCACTGACCGTGATATGATTGCATCCCTTGAAAAAGCGTCTGCTCTTATTACAGAAGAAG
GCGGTTTGACTAGCCATGCTGCGGTAGTCGGATTAAGCCTTGGCATCCCGGTTATCGTGGGTCTGGAAAATGCGACATCT
ATTTTGACAGATGGCCAGGATATTACAGTTGACGCTTCCAGAGGCGCAGTCTATCAAGGCCGTGCGAGCGTTCTTTAA

Protein sequence :
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHTMENG
GIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGSTILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNV
PGVSVNLPGITEKDARDIVFGIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGSY
PVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAIGQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNDSISRKLALVSGVFAESGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
ILTDGQDITVDASRGAVYQGRASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-117 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_007534905.1 pyruvate kinase Pyk VFG0479 Protein 1e-117 53