Gene Information

Name : dhbE (BAM5036_2819)
Accession : YP_007498492.1
Strain : Bacillus amyloliquefaciens UCMB5036
Genome accession: NC_020410
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 3009228 - 3010853 bp
Length : 1626 bp
Strand : -
Note : Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 12354229, 16672620, 9214294; Product type e : enzyme

DNA sequence :
GTGCTGACTGGATTTACGCCATGGCCGGAAGAATGGGCTGACAAATACCGAAAAGACGGCTGCTGGAAGGGTGAGACGTT
CGGAGGAATGCTGAAAGAGCGCGCGGCTTTATACGGAAATCAAACTGCCGTGACGTGTAAAGATGTACATTGGACCTACA
AAGAATTAGATGAAAGGGCAGATCGTTTAGCGGCGGGGCTGCATAAGCTCGGAATTCAAAAGGAAGACCGCGTTGTCGTT
CAGCTGCCGAATATCGCTGAGTTTTTTGAAGTGATTTTCGCGCTGTTCCGGCTGGGAGCGCTGCCCGTTTTCGCGCTGCC
TTCTCATAGAAGCAGTGAGATTACGTATTTCTGCGAGTTTGCAGAGGCGAAAGCATACATCATTCCTGATATACATTCAG
GTTTTGATTACAGGGCGCTTGCCAGACAGGTGAAAGGCAAACTTCCGGATTTGGCGCATGTCATTGTGGCCGGCGAAGCT
GAAGAGTTTCAGGAACTGAGCAGCCTGCATAGAGAGCCTGTTCAGCTGGAAGATATAAGCCCGGCGGAAGTCGCCTTTTT
ACAGCTATCAGGAGGCAGTACGGGGCTGTCCAAGCTGATTCCGCGAACACACGACGATTACATATACAGTTTGCGCATCA
GTGCGGAAATATGCGGCTTGGATCATCACAGCGTTTATTTGGCCGCTCTGCCGACAGCGCATAATTATCCGCTCAGCTCG
CCGGGTGTGCTGGGAACTTTATATGCCGGGGGCCGGGTTGTTCTGTCACCGACGCCGAGTCCGGATGACTGCTTCCCGTT
AATTGAAAAAGAACGTGTCACGATTACAGCGCTCGTCCCGCCGCTTGCGATGGTGTGGATGGACGCATCCGGTTCGCGAA
GTGATGATTTATCAAGCCTTCAAGTGCTGCAGGTGGGAGGCGCCAAATTCAGCGCTGAGGCGGCCCGCCGCGTGAAGAGC
GCTTTCGGATGCACCCTTCAGCAGGTTTTCGGAATGGCGGAAGGATTGGTGAATTATACGAGACTGGATGATCCTGAAGA
GATCATTGTCAATACGCAAGGCAGACCGATGTCTCCTTTTGATGAGGTAAGGGTTTTAGACGATGACGGGCGGGATGTCG
CTCCCGGTGAAGCGGGACATTTGTTTACACGCGGCCCTTACACGATTCGCGGATATTATAAAGCGCCTGAACATAATGAA
AGATCATTCACAGCCGACGGATTTTATCAGACCGGCGATATCGTCAAACTGACGGCGGACGGCTATATCATCGTCGAAGG
ACGTGCGAAAGATCAAATCAATCGGGGCGGTGAAAAAATAGCCGCTGAGGAGGTGGAAAATCACCTTCTTGCGCATCCTG
CCGTTCATGATGCGGCCATGGTTTCAATGCCGGACGATTTTCTCGGGGAGCGATCCTGTGTGTTCGTCATCCCGAAAGGA
GAGGCGCCGAAACCGGGAGAGCTGAAAGCATTTTTGCGTGAGCGCGGACTGGCGGCATATAAAATCCCCGACCGGATTGA
ATTTATCGCTTCTTTTCCGAAAACCGGCGTCGGCAAGGTCAGTAAAAAGGATCTTCGCGAAACGATCGCCCAAAAAGTAA
AGCCGGCTGAAATCAAACAATCTTAA

Protein sequence :
MLTGFTPWPEEWADKYRKDGCWKGETFGGMLKERAALYGNQTAVTCKDVHWTYKELDERADRLAAGLHKLGIQKEDRVVV
QLPNIAEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAKAYIIPDIHSGFDYRALARQVKGKLPDLAHVIVAGEA
EEFQELSSLHREPVQLEDISPAEVAFLQLSGGSTGLSKLIPRTHDDYIYSLRISAEICGLDHHSVYLAALPTAHNYPLSS
PGVLGTLYAGGRVVLSPTPSPDDCFPLIEKERVTITALVPPLAMVWMDASGSRSDDLSSLQVLQVGGAKFSAEAARRVKS
AFGCTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGRPMSPFDEVRVLDDDGRDVAPGEAGHLFTRGPYTIRGYYKAPEHNE
RSFTADGFYQTGDIVKLTADGYIIVEGRAKDQINRGGEKIAAEEVENHLLAHPAVHDAAMVSMPDDFLGERSCVFVIPKG
EAPKPGELKAFLRERGLAAYKIPDRIEFIASFPKTGVGKVSKKDLRETIAQKVKPAEIKQS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 2e-119 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-119 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-119 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-119 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-119 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-119 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-119 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-119 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-119 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
dhbE YP_007498492.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG0168 Protein 5e-143 55
dhbE YP_007498492.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG0932 Protein 1e-117 48
dhbE YP_007498492.1 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E) VFG1817 Protein 7e-114 44