Gene Information

Name : H175_ch2303 (H175_ch2303)
Accession : YP_007478764.1
Strain : Bacillus thuringiensis IS5056
Genome accession: NC_020376
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase [bacillibactin] siderophore
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 2289279 - 2290895 bp
Length : 1617 bp
Strand : +
Note : -

DNA sequence : 
ATGCTAGTAGGTTATACAGAATGGCCAAAAGAATTTGCCGATCATTATCGAGAAGAAGGATGTTGGCTTGGTGAAACATT
TGGAGGAGTGTTAAGAGAGCGGGCTGAGAAATATGGAGATCGAATCGCGGTTGTAAGTGGTAAGAAGCATATAACATATA
GTGAACTTGATAAAAATGCAGATCGTTTAGCAGCAGGTTTACTGAATTTAGGAATAAAGAAAGAGGACAGAGTTGTAATC
CAGTTACCTAACATTATCGAGTTTTTCGAAATATGTTTTGCACTATTTCGAATTGGAGCGCTTCCTGTCTTTGCACTACC
TTCACATCGAAGCAGTGAAATTAGTTATTTTTGTGAGTTTGGCGAGGCGAGCGCTTACGTTATTTCAGATAAGGCTCTCG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTGCCAACTTTACAACGTGTAATTGTAGTGGGGGAAGAA
GAAGAGTTTGTGAACATAAATGATCTGTATATGGATCCCGTCTCATTACCAGAAGTTCAGCCAAGTGATGTTGCATTTCT
CCAATTATCAGGAGGGACAACAGGTCTTTCTAAATTAATTCCTAGAACACATGATGACTATATTTATAGTTTACGTGTAA
GCGCTGAAATTTGTAATTTGAATGCAGAAAGCGTCTATATGGCAGTTCTTCCAGTAGCACACAATTACCCAATGAGTTCT
CCAGGGACATTTGGAACTTTCTATGCGGGTGGAAAAGTGGTATTGGCAACTGGTGGTAGTCCAGATGAGGCATTTGCTCT
TATTGAAAAAGAAAAAGTTACAATTACAGCGCTCGTTCCGCCATTAGCAATGATTTGGCTTGATGCTGCATCTTCTCGTA
ATACCAATTTATCGAGCCTAGAGGTTATTCAAGTAGGTGGTGCTAAGTTTAGCGCTGAGGTTGCAAGACGTATACGCCCT
ACATTTGGATGTACGTTACAGCAAGTATTCGGTATGGCGGAAGGATTAGTGAACTATACAAGATTAGACGATCCGGAAGA
AATTATTATTCATACACAAGGTAGACCAATGTCTGCACTCGATGAAGTACGAGTTGTTGATGAAAATGACAACGATGTAC
AACCTGGCGAAGTAGGTAGTTTATTAACAAGAGGTCCATATACAATTCATGGTTACTATAAAGCTGAAGAGCATAATGCA
CGATCATTTACAAAGGATGGATTTTATCGCACAGGTGATCTTGTAAAAGTAAATGAACAAGGATACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAACCGTGGTGGTGAGAAAGTTGCTGCGGAAGAAGTTGAAAATCATTTATTAGCACACGATG
CGGTCCATGACGTAGCAATTGTATCTATGCCTGACGATTATTTAGGTGAACGCACCTGTGCTTTTGTTATAGCCCGCGGA
CAAGTTCCGGCAGTAAGTGAATTAAAAATGTTTTTAAGAGAACGTGGTATAGCGGCTTATAAAATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGAGTCGGAAAAGTCAGCAAAAAAGAACTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLVGYTEWPKEFADHYREEGCWLGETFGGVLRERAEKYGDRIAVVSGKKHITYSELDKNADRLAAGLLNLGIKKEDRVVI
QLPNIIEFFEICFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQRVIVVGEE
EEFVNINDLYMDPVSLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLNAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNTNLSSLEVIQVGGAKFSAEVARRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDENDNDVQPGEVGSLLTRGPYTIHGYYKAEEHNA
RSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QVPAVSELKMFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-119 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-118 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 9e-119 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 50
ybtE CAA21394.1 - Virulence HPI Protein 9e-119 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-119 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-119 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
H175_ch2303 YP_007478764.1 2,3-dihydroxybenzoate-AMP ligase [bacillibactin] siderophore VFG0168 Protein 1e-135 52
H175_ch2303 YP_007478764.1 2,3-dihydroxybenzoate-AMP ligase [bacillibactin] siderophore VFG0932 Protein 3e-112 47
H175_ch2303 YP_007478764.1 2,3-dihydroxybenzoate-AMP ligase [bacillibactin] siderophore VFG1817 Protein 3e-111 45