Gene Information

Name : H045_00170 (H045_00170)
Accession : YP_007395606.1
Strain : Pseudomonas poae RE*1-1-14
Genome accession: NC_020209
Putative virulence/resistance : Virulence
Product : type III secretion ATP synthase RscN
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 41183 - 42541 bp
Length : 1359 bp
Strand : +
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
GTGAGTACGGACCTGCAGGCGCGCCTGGAGGCCTGGGAGCAGCGCCAGACTCGGTCGCTGGCCAACTTCATGCCGGTGAC
GATGCGCGGTCGAGTGCAGCGCGTGAATGGCATGCTGTTGCAATGCCGTTTGCCCAACGCGCGTATCGGTGACCTGTGCC
AGGTTGAGAAGTCTGTCGACGACCACATGCTGGCTGAGATCATCGGTTTCGATCAGCAGGACGCCGTACTCAGTGCTCTG
GGCAATCTCGAAGGGGTACGCGTGGGCGCCAGCGTGCAGCGTCTGGGGGTGTCGCATCGAGTGCTGGTCGGTGATGAGCT
TCTGGGTCAGGTGCTGGATGGTTTTGGACGGCCGATCAGCGGCGTTGGCCCCAGTGCGTTTGTCGAGGTCGACAGTCCCG
ATGCAAGCATGGTGTTGTGCGAGGCGCCGCTGCCTACCGAACGGCCCAGGATCCATCGCGCACTGGCGACCGGGGTGCGC
TCGATCGATGGCCTGCTGACGCTTGGCGAGGGGCAGCGTGTGGGGCTGTTTGCCGGTGCCGGCTGTGGCAAGACCACCTT
GCTGGCGGAGATTGCCCGTAACGTCGAGTGCGATGTGATTGTGTTTGGCTTGATCGGCGAGCGGGGACGCGAACTGCGCG
AGTTTCTCGACCACGAGCTGGATGACCAGTTGCGCGCCAAGGCCGTGCTGGTGTGCGCGACATCCGATCGTTCAAGCATG
GAGCGCGCCCGCGCAGCCTTCACCGCGACCGCGCTGGCTGAGGGCTATCGGCGCAAGGGCAAGCGGGTGCTGCTGCTGAT
CGACTCCCTGACCCGGTTTGCGCGGGCCCAGCGCGAAATCGGTCTGGCGGCCGGTGAGCCGTTGGGGCGAGGTGGCCTGC
CACCCTCGGTTTACAGCTTGCTGCCGCGACTGGTGGAGCGCGCCGGGCTGACCCAGAACGGCGTTATAACGGCGATCTAC
ACCGTGCTTATCGAGCAGGACTCCATGAGCGATCCCGTGGCGGACGAAGTCCGCTCGCTGCTCGATGGCCACATTGTTCT
GTCCCGCAAGCTGGCCGAGCGTGGTCACTACCCGGCCGTTGATGTGCTGGCCAGCCTGTCGCGAATTTTGAGCAACGTCG
CCACGCCCGAACATATTCAGGCCGGTACGGCACTGCGGCGCTTGTTGTCGGCGTACCAGCAGATCGAGCTGATGCTGAAG
TTGGGCGAGTACCAGCCTGGCAGTGACGCCCTGACCGACATGGCGGTGGACACCCGTTCGCGGGTCGATGGTTTTCTGCG
TCAGGACTTGCGCGAGCCCGCGCTGATGGACTCGACCCTGGATCAATTGACGGAGCTTACTGCCCATGTCCCTTTCTGA

Protein sequence :
MSTDLQARLEAWEQRQTRSLANFMPVTMRGRVQRVNGMLLQCRLPNARIGDLCQVEKSVDDHMLAEIIGFDQQDAVLSAL
GNLEGVRVGASVQRLGVSHRVLVGDELLGQVLDGFGRPISGVGPSAFVEVDSPDASMVLCEAPLPTERPRIHRALATGVR
SIDGLLTLGEGQRVGLFAGAGCGKTTLLAEIARNVECDVIVFGLIGERGRELREFLDHELDDQLRAKAVLVCATSDRSSM
ERARAAFTATALAEGYRRKGKRVLLLIDSLTRFARAQREIGLAAGEPLGRGGLPPSVYSLLPRLVERAGLTQNGVITAIY
TVLIEQDSMSDPVADEVRSLLDGHIVLSRKLAERGHYPAVDVLASLSRILSNVATPEHIQAGTALRRLLSAYQQIELMLK
LGEYQPGSDALTDMAVDTRSRVDGFLRQDLREPALMDSTLDQLTELTAHVPF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-129 68
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-143 67
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 4e-144 67
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-143 67
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-130 67
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-136 65
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 3e-137 65
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-137 65
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 7e-142 65
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 6e-129 64
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 6e-129 64
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-136 64
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-97 52
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-85 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-85 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-84 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-84 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-85 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-84 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-84 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-86 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-85 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 6e-86 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 6e-86 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-85 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-88 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-84 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-84 49
escN CAI43874.1 EscN protein Virulence LEE Protein 8e-81 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-80 46
escN AAK26715.1 EscN Virulence LEE Protein 2e-80 46
escN AAL57542.1 EscN Virulence LEE Protein 2e-80 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-80 46
escN AAC38383.1 EscN Virulence LEE Protein 4e-79 45
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-78 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-80 45
escN AAC31513.1 L0034 Virulence LEE Protein 3e-79 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-79 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-79 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-79 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-79 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-80 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-80 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-65 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-71 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 6e-66 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 5e-67 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0192 Protein 2e-97 54
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0390 Protein 1e-92 53
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0048 Protein 3e-93 50
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0515 Protein 1e-84 50
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG2144 Protein 3e-84 46
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0730 Protein 2e-79 45
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0812 Protein 2e-79 45
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG1254 Protein 4e-71 44
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG1778 Protein 1e-69 43
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG2499 Protein 1e-70 43
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG0555 Protein 3e-71 42
H045_00170 YP_007395606.1 type III secretion ATP synthase RscN VFG1008 Protein 6e-69 41