Gene Information

Name : D781_3942 (D781_3942)
Accession : YP_007346317.1
Strain : Serratia marcescens FGI94
Genome accession: NC_020064
Putative virulence/resistance : Virulence
Product : type III secretion apparatus H+-transporting two-sector ATPase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 4157495 - 4158862 bp
Length : 1368 bp
Strand : -
Note : PFAM: ATP synthase alpha/beta family, beta-barrel domain; ATP synthase alpha/beta family, nucleotide-binding domain; TIGRFAM: ATPase FliI/YscN family; type III secretion apparatus H+-transporting two-sector ATPase

DNA sequence :
ATGACGGAGATCGCCGGCGGGATCGCCGCCTGGGCCGATCGTCAGCGGCGGCGCCTGCAGGCGGCGGACGCGGTGACGGC
CTACGGCCGGGTGACCGGCATCAGCGGCATCCTGCTGGAGTGCAGCCTGCCGCGCGCGCGCATCGGCGATCTGTGCCGCA
TCACGCGCGGTGAGCAGGAGAGCGTGCTGGCGGAGGTGGTGGGTTTTAACCCGCAGCATACGCTGCTGTCGGCGCTGGGG
CCGCTGGACGGCATTGCCCGCGGCGCGCGGGTGACGCCGCTGTGGCTGCCGCACAGCATCAGCGTGTCCGAGGCGCTGCT
CGGCAGCGTGCTGGACGGCTTCGGCCGGCCGCTGGATGAACAGAGCGTCGGCGCCTTTGCGCTGCCGGGGAGCGGCGTTG
ATACCGTGCCGGTGGTGAGCGACGCGCCGCCGCCGACCGCGCGTCCGCGCATTGCCCAGGCGCTGCCGACCGGCGTGCGC
GCCATCGACGGTCTGTTGACCCTCGGCGTCGGCCAGCGGGTCGGCGTGTTTGCCGGCGCCGGCTGCGGCAAAACCACGCT
GCTGGCGGAGCTGGCGCGCAATACCCCCTGTGACGCCATCGTTTTCGGCCTGATCGGCGAGCGCGGCCGCGAGCTGCGTG
AGTTCCTCGACCATGAGCTGGATGAAGCGCTGCGCAGCCGCACGGTGCTGGTGTGCGCCACCTCGGATCGCAGCAGCATG
GAGCGCGCCCGCGCGGCCTTTACCGCCACGGCGATTGCCGAAGCGTTCCGCGCGCAGGGAAAAAGCGTGCTGCTGATCCT
CGACTCGCTGACCCGCTTCGCCCGCGCCCAGCGTGAAATCGGCCTGGCGCTGGGGGAGCCGCCGGGGCGCGGCGGGCTGC
CGCCTTCGGTGTATACCCTGTTGCCGGGGCTGCTGGAGCGGGCGGGGCAGACCATGACGGGCAGCATCACCGCCCTGTAT
TCGGTGTTGATTGAAGCGGATTCGATGAACGACCCGGTGGCGGACGAAGTGCGCTCGCTGATCGACGGCCATATTGTGCT
GACCCGCCGGCTGGCAGAGCGCGGCCACTATCCGGCCATCGACGTGCTGGCCAGCCTCAGCCGCACCATGAGCAACGTGG
CGGAGCGGGAGCAGCTAACCGACGCCACCCGGTTGCGCCGCCTGATGTCGGCCTGGCAGCAGGTGGAGATGCTGATCCGG
CTGGGGGAGTACGAGCCGGGGCACGACGCGATGACCGACGCCGCGGTGGAGGCGCAGGGCGCCATCAACGGTTTTTTACG
CCAGGAGACGCGCGCCCCCTGCGCCTGGGAAGAGACTTTGCAGCAACTGACGGAGGTAAGCGGCTATGCGCCGGAACAGC
AACATTGA

Protein sequence :
MTEIAGGIAAWADRQRRRLQAADAVTAYGRVTGISGILLECSLPRARIGDLCRITRGEQESVLAEVVGFNPQHTLLSALG
PLDGIARGARVTPLWLPHSISVSEALLGSVLDGFGRPLDEQSVGAFALPGSGVDTVPVVSDAPPPTARPRIAQALPTGVR
AIDGLLTLGVGQRVGVFAGAGCGKTTLLAELARNTPCDAIVFGLIGERGRELREFLDHELDEALRSRTVLVCATSDRSSM
ERARAAFTATAIAEAFRAQGKSVLLILDSLTRFARAQREIGLALGEPPGRGGLPPSVYTLLPGLLERAGQTMTGSITALY
SVLIEADSMNDPVADEVRSLIDGHIVLTRRLAERGHYPAIDVLASLSRTMSNVAEREQLTDATRLRRLMSAWQQVEMLIR
LGEYEPGHDAMTDAAVEAQGAINGFLRQETRAPCAWEETLQQLTEVSGYAPEQQH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-139 79
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-130 72
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-130 72
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 6e-131 72
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 9e-130 72
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 3e-120 70
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-120 70
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-109 70
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-117 69
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-119 68
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-107 65
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-107 65
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-75 52
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-75 52
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-74 52
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-74 52
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-75 52
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-81 52
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-78 52
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-78 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-79 51
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-79 51
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-80 51
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 4e-80 51
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-80 51
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-80 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-80 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-74 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 5e-71 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 5e-71 47
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-69 47
escN AAC38383.1 EscN Virulence LEE Protein 1e-70 47
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-70 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 8e-70 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-70 47
escN AAC31513.1 L0034 Virulence LEE Protein 6e-70 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-70 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-70 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-70 47
escN AAK26715.1 EscN Virulence LEE Protein 1e-70 47
escN AAL57542.1 EscN Virulence LEE Protein 1e-70 47
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-70 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 8e-70 47
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-60 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 7e-60 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-60 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-60 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-60 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 5e-58 44
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 5e-60 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0390 Protein 3e-80 53
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0048 Protein 3e-83 53
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0192 Protein 1e-82 53
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0515 Protein 1e-75 52
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG2144 Protein 1e-77 48
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG1254 Protein 6e-62 48
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0730 Protein 8e-71 47
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0812 Protein 4e-70 47
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG0555 Protein 3e-60 44
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG1008 Protein 5e-59 44
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG2459 Protein 6e-64 44
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG1778 Protein 2e-59 44
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG2499 Protein 1e-60 44
D781_3942 YP_007346317.1 type III secretion apparatus H+-transporting two-sector ATPase VFG2331 Protein 5e-65 43