Gene Information

Name : Mycsm_03069 (Mycsm_03069)
Accession : YP_007292793.1
Strain : Mycobacterium smegmatis JS623
Genome accession: NC_019966
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase (class II)
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 3012932 - 3016228 bp
Length : 3297 bp
Strand : -
Note : PFAM: tRNA synthetases class II (D, K and N); Uncharacterized conserved protein (DUF2156); OB-fold nucleic acid binding domain; TIGRFAM: lysyl-tRNA synthetase, eukaryotic and non-spirochete bacterial

DNA sequence :
ATGACCGCCACGTTGACGCGTCGGGCCTCCCGCTACTCATGGGTGCCCGCAGCCGCCGGCTGGACGGTCGGCATCATTGC
CACGCTGTCGTTGATCGCCAGCGTCTCACCGGGAATCCGGTGGGTGATCAAGGAGCCCCGCGAGTTCGTCAACAACTACA
TCTTCAACTTCCCCGACACCAGCTTCGCGTGGGCCTTCGTGCTGGCGCTGCTCGCGGCGGCGTTGGCAGCCCGCAAGCGG
ATCGCATGGTGGATCCTCATCGGCTACATGGTCGCGTCGATCGGGTGGAACGTCGGCGGATTGGTCACGAGCCCCAAGTC
GGTGATGGACGACATCGGCGAAGTGATCGGGGTGGCGTTCCACGTCACGTCGATCCTGTGCCTGGTCCTGGCCCGCAAGG
AATTCTGGGCGAAGGTGCGCCGCGGAGCATTGCTGAAGGCGGTGTTCACGCTGGTCGCGGCCATGGGAATCGGCACCCTC
ATCGGCTGGGCCCTACTCGAGGTGTTCCCTGGCAGCCTGGCCCGCGAAGACCGCTTCCTCTACGCGCTCAACCGGGTCAG
CGCCTTCGCGGGCGCCGACTCCGCCTCGTTCAGCGGCCACCCGCATGTCTTCGTCAACGCGCTGCTCGGCCTGTTCGGCG
CGGTCGCGCTGATGGTGACCGCGATCGTGCTGTTCCAGTCGCAGCGCGCCGACAACGCGCTCACCGGCGAGGACGAATCA
GCCATCCGCGGGCTGTTGGAACTGTTCGGAAAGAACGACTCACTTGGCTATTTCGCCACCCGCCGCGATAAGGCCGTGGT
CTTCGCCTCCAACGGCCGGGCGGCCATCACCTATCGCGTCGAGGTCGGCGTCTGCCTCGCCAGCGGCGACCCCGTCGGCG
ATCCGAAGGCGTGGCAGCAAGCCATCCATGCGTGGCTAGAGCTGTGCCAGACGTACGGCTGGGCGCCCGGCGTGATGGGG
GCCAGCTCGACGGGCGCGGAGGCGTTTCGGCAGGCGGGCCTCAACGCGCTGCAACTCGGCGACGAGGCCATCCTGTATCC
CGACAGCTTCCGGCTCTCCGGACCCGAAATGCGGGCAGTGCGCCAGGCCGTGACGCGGGCGCGACGGGCAGGTGCAACCG
TCCGGATTCGACGGCACCGAGATCTGTCCAGCGAGGAAATGGCCAAGGTCATCGTGCGTGCGGACACGTGGCGCGACACC
GAAACCGAGCGCGGCTTCTCGATGGCGCTTGGCAGACTCGGCGACTCCGCTGACGGCGACTGTCTGCTGGTGGAAGCTGT
GCAAAACGATCAAGTCGTGGCGATGCTGTCGCTGGTCCCGTGGGGCAACAACGGCGCGTCGCTCGACCTGATGCGCCGCT
CACCGCAGTCCCCCAACGGAACCATCGAATTGATGGTCAGCGAGCTATGCATGCAATCCGAAGGCATTGGGGTGACCCGG
ATTTCGCTGAACTTCGCGATGTTCCGGACGGCATTCGAACACGGCGCACAGCTCGGGGCGGGCCCTGTCGCGCGGCTGTG
GCGTGGCCTGTTGGTGTTCTTCTCCCGGTGGTGGCAGCTCGAGACGCTGTACCGGTCGAACATGAAATACCTGCCGCAGT
GGGTGCCGCGGTACGCCTGCTACGAAGACGCCCGCCTTGTGCCCCGCGTCGGTGTCGCATCGGTGATCGCCGAAGGCTTC
CTGGTGCTGCCGTTCTCGCGACGCGCTGAGCAACCGCATACCGGCCATCACATCTCGGCTCCCGAAAACCTGGTCGCGAC
AGGGCTTTTGCGCCATGACGGCAGCGCGCCCGACCTCGGCGAGCTAGAGGCCGACCTCCCCGAGGCCGACGACGGAGCGC
GACTGCCTGAGCAGGTGCGCGTCCGGATGGCCAAGTTGAAGTCGTTGCAAGACAACGGCGTCGACGCATACCCCGTCGGC
GAGGCGCCCAGCCACACCGTCGCGCAGGCAGTCGACTCCGAGGGCGCAGGCACCGTGACCGTCGCAGGGCGGGTGCTTCG
CATCCGCGACTACGGCGGCGTGCTGTTCGCGCAGTTGCGCGACTGGTCGGGCGAAGTGCAGCTGCTGCTTGACAATTCGA
TCCTTGAAGACGGATCTACCGCGGATTTCACCCAGGCCATCGATCTTGGCGACCTGATCCAGGTGTCCGGGACCATGGGC
TACAGCAAGAAGGGCACCCGCTCGCTGCTTGTTCTCAGTTGGCGACTGATCGGCAAGTGCCTGCGCCCGCTGCCCGATAA
GTGGAAGGGGCTGACCGACCAGGAGTCGCGGGTACGGGCCCGCTACGTCGACCTGGCGATCAACACCGAGGCCCGCGAGC
TGATCCGAGCGCGCAGCGGGGTGCTGCACGCGATCCGCGAAACCCTGGTCGGCAATGGGTTTTTGGAGGTCGAAACCCCG
ATCCTGCAGCAGATCCACGGCGGTGCCAACGCCAGGCCGTTCCTCACCCACATCAACGCCTACGATCTCGATCTGTATCT
GCGCATCGCGCCCGAGCTGTATCTCAAGCGGCTGTGCGTCGGCGGTGTCGAGCGGGTCTTCGAGCTTGGTCGCGCGTTTC
GCAACGAGGGTGTCGACTTCAGCCACAACCCCGAGTTCACACTGCTGGAGGCCTATCAGGCGCACGCCGACTACAACGTC
TGGATCGGTGGCTGCCGCGAGCTGATCCAGAACGCCGCGCAGGCCGCCAACGGCGCACAGGTCTTCATGCGTCCTCGCGA
GGACGGCACGCTGGAACCCGTCGACATCTCCGGGGAGTGGACGGTGAAGACCGTGCACGGCGCGGTGTCCGAGGCGCTCG
GCGAGCAGATCGGGCCCGACACGAATCTGGCCGTGCTGCGCAAGCTTTGCGATGCGGCAGGCATCCCGTACCTGACCCAC
TGGGATGCGGGCGCGGTGGTGCTCGAGCTTTACGAACGGTTGGTCGAGGACCGTACCGAGGCGCCGACTTTCTACAAGGA
CTTCCCCACCTCGGTATCGCCGCTGACCCGGCCGCATCGCAGCATCCCCGGGGTCGCCGAGCGCTGGGATCTGGTGGCGT
GGGGGGTGGAGTTGGGCACCGCCTACAGCGAGCTGACGGATCCCGTCGAGCAGCGCCGACGGCTGCAGGAGCAGTCGCTG
CTGGCCTCGGGCGGCGATCCCGAGGCGATGGAACTCGACGAAGACTTCCTGCAGGCAATGGAGTACGCGATGCCGCCGAC
GGGCGGCCTCGGCATGGGTGTCGACCGGGTGGTCATGCTGATTACCGGACGCAGCATCCGCGAAACGCTGCCGTTCCCCT
TGGCCAAACCGCGCTAG

Protein sequence :
MTATLTRRASRYSWVPAAAGWTVGIIATLSLIASVSPGIRWVIKEPREFVNNYIFNFPDTSFAWAFVLALLAAALAARKR
IAWWILIGYMVASIGWNVGGLVTSPKSVMDDIGEVIGVAFHVTSILCLVLARKEFWAKVRRGALLKAVFTLVAAMGIGTL
IGWALLEVFPGSLAREDRFLYALNRVSAFAGADSASFSGHPHVFVNALLGLFGAVALMVTAIVLFQSQRADNALTGEDES
AIRGLLELFGKNDSLGYFATRRDKAVVFASNGRAAITYRVEVGVCLASGDPVGDPKAWQQAIHAWLELCQTYGWAPGVMG
ASSTGAEAFRQAGLNALQLGDEAILYPDSFRLSGPEMRAVRQAVTRARRAGATVRIRRHRDLSSEEMAKVIVRADTWRDT
ETERGFSMALGRLGDSADGDCLLVEAVQNDQVVAMLSLVPWGNNGASLDLMRRSPQSPNGTIELMVSELCMQSEGIGVTR
ISLNFAMFRTAFEHGAQLGAGPVARLWRGLLVFFSRWWQLETLYRSNMKYLPQWVPRYACYEDARLVPRVGVASVIAEGF
LVLPFSRRAEQPHTGHHISAPENLVATGLLRHDGSAPDLGELEADLPEADDGARLPEQVRVRMAKLKSLQDNGVDAYPVG
EAPSHTVAQAVDSEGAGTVTVAGRVLRIRDYGGVLFAQLRDWSGEVQLLLDNSILEDGSTADFTQAIDLGDLIQVSGTMG
YSKKGTRSLLVLSWRLIGKCLRPLPDKWKGLTDQESRVRARYVDLAINTEARELIRARSGVLHAIRETLVGNGFLEVETP
ILQQIHGGANARPFLTHINAYDLDLYLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYNV
WIGGCRELIQNAAQAANGAQVFMRPREDGTLEPVDISGEWTVKTVHGAVSEALGEQIGPDTNLAVLRKLCDAAGIPYLTH
WDAGAVVLELYERLVEDRTEAPTFYKDFPTSVSPLTRPHRSIPGVAERWDLVAWGVELGTAYSELTDPVEQRRRLQEQSL
LASGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRVVMLITGRSIRETLPFPLAKPR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 6e-153 42
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-153 42
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-153 42
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-153 42