Gene Information

Name : Cyast_0517 (Cyast_0517)
Accession : YP_007164145.1
Strain : Cyanobacterium stanieri PCC 7202
Genome accession: NC_019778
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.1.40
Position : 577257 - 579035 bp
Length : 1779 bp
Strand : -
Note : PFAM: Pyruvate kinase, barrel domain; PEP-utilising enzyme, mobile domain; Pyruvate kinase, alpha/beta domain; TIGRFAM: pyruvate kinase; COGs: COG0469 Pyruvate kinase; InterPro IPR015793:IPR015794:IPR008279:IPR001697; KEGG: cyc:PCC7424_3440 pyruvate kinas

DNA sequence :
ATGTCTGAGAAATTTTCCCGCCGTACCAAAATTGTAGCAACCGTAGGCCCAGGTTGTGCGAATCCCGAAACATTGCGCAA
GATGATTCTTGCTGGCGCTAATACTTTTCGTCTCAACTTTTCCCATGGTACCCATGAAGTCCATCAAAGTAGTATTCGGA
TGATTCGCCAAGTGGAAAATGAGTTAAATCGTCCCATCGGTATTTTGCAAGATTTACAAGGTCCGAAAATTAGGTTAGGT
AAATATGAATGTGGTTCTATTGAATTACATGAGGGCGATCGCTACGTACTCACCAGTCGAGATGTCAAATGTGATGAAAA
AATCGCCTGTATTAGTTACGAATATTTAGCCGAAGAAGTACCCCTCAATGCCAGAATCCTCCTTGATGATGGTAGGGTGG
AAATGGTAGTAAAGGAAATTGATAAAGAAAATAAAGATCTCCATTGTCAGGTAGTAGTAGGGGGTGTACTGTCTAGCAAC
AAAGGGGTAAACTTTCCCAATGTTTATTTATCCGTCAAAGCCCTAACGGAAAAAGACAAAAAAGATTTAATGTTTGGTTT
AGATCAACGGGTGGACTGGGTAGCCCTCAGTTTCGTGAGAAATCCCCAAGATGTGTTGGAAATCAAAGATTTAATTGCCA
GTGCAGGAAAATCTACCCCCGTCATTGCCAAAATTGAAAAACACGAAGCCATCGAACAAATGGAAGAAATTTTATCCCTC
TGTGATGGGGTAATGGTGGCAAGGGGAGATTTAGGGGTTGAGTTACCCGCTGAGGATGTGCCTATCCTCCAAAAACGCTT
GATTCGCACTGCCAACCGTTTGGGTATTCCCATTATTACCGCTACCCAAATGTTAGACAGTATGGCAAACAGTCCTAGTC
CTACCCGTGCAGAGGTTTCCGATGTTGCCAACGCCATTTTAGACGGCACCGATGCGGTAATGCTTTCCAATGAAACCGCT
GTGGGTAAATATCCTGTACAAGCTGTGGCTACCATGGCAAAAATTGCTAGACGCACGGAGGAAGAAAGGGATTCCATTGC
CCCCCATTTGTTGAGTCCTATGGATAACCAAAATATTCCTAATGCCATTTCTGGGGCGGTGGGACAAATCGCCAAACAGC
TTAAGGCTAGTGCCATCATGACCCTAACGAAAACGGGTGCTACGGCTCGTAACGTCTCCAAATTTCGTCCTAAAACTCCC
ATTTTGGCAATTACCCCCCATGTCAGTGTTTCCCGTCAATTACAGTTGGTCTGGGGTGTGAAACCTTTATTGTTGTTGGA
TATGCCGGGCTTAAAGCAGGTGTTTAGTTCGGCTATCGAGTTGGCGAGGGAAGAGGAGTTATTAGAAGATGGTAATTTAG
TGGTGATGACCGCAGGAACTTTGCAAGGGGTGGCAGGTTCTACCGATTTGATTAAGGTAGAAATTGTTAAGGGTTTACTC
AGTGAAGGGCTTGGTATCGGACAGGGGATAATTACTGGTCGCACTAAGGTGGTTTATGATATTAACAATCTCACCAATTT
TAATCAGGGCGATATTTTGGTGGCAAAAAGCACTGATAATAATTATGTAGATGCTATGCGCCTAGCCAGTGGCATTATTA
CCGAAGAGGGTGGGGTGCGATCGCACGCAGCCCAGATAGGAATGCGTTTAGGTATTCCCGTAATTGTGGGAGTAAAAGAT
GCCACCCGAATCATTCGGGATGCTAGTTTTGTCACCATGAAAATTGAACAGGGTTTGGTCTATCTTGGTACTGATGGCAG
TCAAGATGGTGTAGATTAA

Protein sequence :
MSEKFSRRTKIVATVGPGCANPETLRKMILAGANTFRLNFSHGTHEVHQSSIRMIRQVENELNRPIGILQDLQGPKIRLG
KYECGSIELHEGDRYVLTSRDVKCDEKIACISYEYLAEEVPLNARILLDDGRVEMVVKEIDKENKDLHCQVVVGGVLSSN
KGVNFPNVYLSVKALTEKDKKDLMFGLDQRVDWVALSFVRNPQDVLEIKDLIASAGKSTPVIAKIEKHEAIEQMEEILSL
CDGVMVARGDLGVELPAEDVPILQKRLIRTANRLGIPIITATQMLDSMANSPSPTRAEVSDVANAILDGTDAVMLSNETA
VGKYPVQAVATMAKIARRTEEERDSIAPHLLSPMDNQNIPNAISGAVGQIAKQLKASAIMTLTKTGATARNVSKFRPKTP
ILAITPHVSVSRQLQLVWGVKPLLLLDMPGLKQVFSSAIELAREEELLEDGNLVVMTAGTLQGVAGSTDLIKVEIVKGLL
SEGLGIGQGIITGRTKVVYDINNLTNFNQGDILVAKSTDNNYVDAMRLASGIITEEGGVRSHAAQIGMRLGIPVIVGVKD
ATRIIRDASFVTMKIEQGLVYLGTDGSQDGVD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 1e-61 43
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-72 42
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-72 42
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 5e-73 42
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 8e-72 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Cyast_0517 YP_007164145.1 pyruvate kinase VFG0479 Protein 8e-73 42