Gene Information

Name : fliI (PputUW4_03875)
Accession : YP_007030872.1
Strain : Pseudomonas sp. UW4
Genome accession: NC_019670
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 4467357 - 4468715 bp
Length : 1359 bp
Strand : -
Note : [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGCGCCTTGAACGCACCAGCTTCGCCAAGCGCCTGGGCAGCTACGCCGAGGCCACGGGACTGGAGGGCGCACCGATCCT
CGAAGGCCGCCTGCTGCGCATGGTCGGCCTGACCCTCGAAGCCGAAGGCCTGCGCGCCGCCATGGGCAGTCGCTGCATGG
TCATCAATGGCGACAGCTATCACCCGGTGCAGGTGGAAGCCGAGGTCATGGGCTTCTCTGGCAGCAAAGTCTTTCTGATG
CCGGTCGGCAGTGTCGCCGGCATCGCACCCGGCGCCCGAGTGGTGCCGTTGGCGGACAACGGTCGCCTGCCCATGGGCAT
GAGCATGCTCGGAAGGGTGCTCGACGGTGCCGGTCGTGCACTGGACGGCAAGGGCGGGATGAAGGCCGAAGACTGGGTGC
CGATGGATGGCCCGACCATCAACCCGCTCAAGCGCGAGCCCATCAGTGAACCGCTGGACGTGGGCATCCGTTGCATCAAC
GGCATGTTGACGGTCGGCCGCGGCCAGCGGCTCGGCCTGTTCGCCGGTACCGGTGTCGGTAAATCGGTGTTGCTGGGGAT
GATGACGCGCTTTACCGAGGCCGACATTATCGTCGTCGGTCTGATCGGTGAGCGGGGTCGTGAGGTCAAGGAATTCATCG
AGCACATTCTCGGTGAAGAAGGACTCAAGCGTTCAGTGGTCGTGGCGTCCCCGGCGGACGATGCGCCGCTGATGCGACTG
CGCGCCGCCATGTACTGCACGCGCATCGCCGAATATTTCCGCGACAAGGGCAAGAATGTCCTGTTGCTCATGGACTCTCT
GACCCGTTTCGCCCAGGCCCAGCGGGAAATCGCCCTGGCCATCGGCGAGCCACCGGCGACCAAGGGCTATCCGCCATCGG
TGTTCGCCAAACTACCGAAACTGGTGGAGCGGGCCGGTAACGCAGAGAAGGGCGGCGGTTCGATCACCGCGTTCTACACC
GTGCTGTCCGAAGGCGACGACCAGCAGGACCCGATTGCCGACTCGGCACGGGGTGTACTCGACGGGCACATCGTGCTCTC
CCGGCGCCTGGCCGAGGAGGGGCATTACCCGGCCATCGATATCGAAGCGTCCATCAGCCGGGTCATGCCGGCGGTGGTGT
CGCCCGAGCACATGGTTCGTGCGCAATATTTCAAGCAGCTGTGGTCGCGTTATCAACAGAGTCGCGACCTGATCAGCGTC
GGCGCCTATGTCGCCGGCGGCGATCGTGAGACCGACCTGGCGATTTCCCTGCAACCGCAATTGGTCAAATACCTGCGCCA
GGGCCTCAACGACAGCATCAGCCTGGGTGAAAGCGAAGCGTATCTCGCCTCGATCTTCGCGCCCGCGGCGGGCGGGTAA

Protein sequence :
MRLERTSFAKRLGSYAEATGLEGAPILEGRLLRMVGLTLEAEGLRAAMGSRCMVINGDSYHPVQVEAEVMGFSGSKVFLM
PVGSVAGIAPGARVVPLADNGRLPMGMSMLGRVLDGAGRALDGKGGMKAEDWVPMDGPTINPLKREPISEPLDVGIRCIN
GMLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEADIIVVGLIGERGREVKEFIEHILGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATKGYPPSVFAKLPKLVERAGNAEKGGGSITAFYT
VLSEGDDQQDPIADSARGVLDGHIVLSRRLAEEGHYPAIDIEASISRVMPAVVSPEHMVRAQYFKQLWSRYQQSRDLISV
GAYVAGGDRETDLAISLQPQLVKYLRQGLNDSISLGESEAYLASIFAPAAGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-78 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-78 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-79 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-78 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-87 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-87 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-87 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-84 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-73 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-83 48
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-72 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-86 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-86 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-78 47
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-72 47
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-72 47
escN CAI43874.1 EscN protein Virulence LEE Protein 5e-74 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 6e-74 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-70 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-67 46
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-85 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-85 46
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-73 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-74 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-70 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 7e-71 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-75 45
escN AAC38383.1 EscN Virulence LEE Protein 7e-75 45
escN AAC31513.1 L0034 Virulence LEE Protein 4e-75 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-75 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-75 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-70 44
escN AAK26715.1 EscN Virulence LEE Protein 2e-73 44
escN AAL57542.1 EscN Virulence LEE Protein 2e-73 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 6e-74 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 7e-74 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-67 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-67 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 8e-68 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_007030872.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 87
fliI YP_007030872.1 flagellum-specific ATP synthase VFG2499 Protein 2e-125 63
fliI YP_007030872.1 flagellum-specific ATP synthase VFG2331 Protein 2e-121 62
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0515 Protein 8e-79 49
fliI YP_007030872.1 flagellum-specific ATP synthase VFG2144 Protein 1e-88 48
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0048 Protein 4e-88 47
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0192 Protein 7e-85 47
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0390 Protein 2e-84 46
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0730 Protein 4e-75 45
fliI YP_007030872.1 flagellum-specific ATP synthase VFG0812 Protein 3e-75 45