Gene Information

Name : M5M_08260 (M5M_08260)
Accession : YP_006916571.1
Strain : Simiduia agarivorans SA1
Genome accession: NC_018868
Putative virulence/resistance : Resistance
Product : acriflavin resistance protein
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 1835252 - 1838386 bp
Length : 3135 bp
Strand : +
Note : COG0841 Cation/multidrug efflux pump

DNA sequence :
ATGAACATCGCCGAGCGGTCCATTAAGAACAGCACGATCACCTGGGTTGTCACCGGTATTTTACTGGTGGTCGGCATGAT
CGCGTTCAACAACCTGAGCCGGCTTGAAGACCCGGAATTCACCATTAAAGAGGCCATGATCATTACCCCTTACCCGGGGG
CCAGCGCGCGCGAGGTAGAGATAGAAGTCTCCGATCTGGTCGAACGCGCCACCCAGGAGATGGGCCAGCTGTTTTATGTG
GAGTCACATTCCTATCGGGATAAAAGTATCGTCAAGGTCCGCATAAAAGACCAATACGACAAAGATGGATTGCCACAGGT
CTGGGATGAGTTGCGCCGAAAGGTCAATGATATCCAGCACCAGCTACCACCCGGCGCTGGACCTTCATTGGTAAACGATG
ACTTCGGTGATGTGTTTGGTGTTTACATCGCACTCACCGGGGACGGCTATACGCAAAAAGACCTGTACGAATTCGCCAAA
TTTCTCCGCCGCGAACTGCTGCTTGTGCAGGATGTAAAGAAAATCACTTTCTATGGCCAACCAAAAGAAGTGATTTACGT
GGAAATGCAACGCGACAAGATGACCGCATTGGGCATTTCACCACAGGATATATACAGTGCGCTGGGCGCCAAAAACACCG
CCGAGCAGGCGGGCTATATGACGGTCGGCAACAGACGTCTGGCGGTCTCCCCCACGGGCGAATTCCACTCCGAACAACAA
TTTGCCGGTCTGGTGATCAACGCCAGAAACTTGCAGAACCAGCAAGTCGTGCTGGGTGACGTTGCCACCATCACCCGGGG
CCTGCAGGACCCGCCCAATAATCTGCTGCGATTTGATGGCGAGCCGGCCATAGGGCTTGCCATTTCTACCACCAGTGGCG
GCAACGTGGTAACTATGGGTGAAGGTCTGTACCAGAAAGTTGAAGAGCTGAAACCGCAGATACCGCTGGGAATCCAATTG
AATGTGGTTTCCATGCAATCTGACAGCGTTGTTGAGGCGATTAATAATTTTCTGATCAGTCTGATTGAAGCGGTGGTGAT
TGTTGTTGTGGTGCTGTTGTTTTTCATGGGCCTGCGCAGCGGCCTGATTATCGGCACCGTGTTGGCAGTCACCATCACCG
GCACCTTTATTTTCATGAGTATCCTGGGCATTACGCTGGAGCGGATCTCCTTGGGCGCATTGATCATTGCGTTGGGCATG
CTGGTCGATAACGCCATCGTGGTCACCGATGGCATGCGGGTGCGAATGGAACAAGGGGAAGACGCCTTGAGCGCGGCACG
CAAAGTCGTTTCTCAGACCGCTATGCCTCTGCTTGGCGCCACTGCGGTGGCAATTGCCGCTTTCGCCGCCATCGGTACAT
CCCAGGATGCCACCGGCGAATACACCCGCAGCTTGTTTACCGTGATTCTGATTTCATTGACCATGAGCTGGGTCACCGCG
GTGACCACCACCCCATTGCTGTGCAAAACATTTTTAAAGAGCGGCCAGTTGGGCAGCCAGGGCGCGCCCTATCAAGGTGT
GTTCTACCAGCGCTACCGGCAGTTTCTCACGCTCGCCATTCAACACCGCTGGGTGGCTATCGGTATCACCGTGGCACTTT
TCGCGGCATCGCTGATTGGGTTTGGCTATGTGAAGCAAAGCTTTTTCCCGGACTCCACCCGTCCGCAATTCATGGTGGAT
CTGTGGTTGCCGGAAGGTACCCACATCCATGAAACCGAACGCAGGCTCGCGCCTTTTGAGGAAAAGCTTAAAGGCTACAG
CAATGTCACCAAGGTCACCACGCAGGTGGGCGGCAGCTCGCCGCGCTTTTTGCTGACCTATCAGCCCGAGCAACCCAACA
GCAATTTTGCCCGCCTGCTAGTGGATGTGGACGATTACCGCATCATCAACCAGTTCAGCCAGCAGGTACAAAATGACCTC
GAATTTATCGACCCGGATGTCAATGTAAACGTGCGTCTGTTTGTGCTTGGCCCGGCAACGGGAGGCAAGGTACAGCTGCG
TTTATCCGGGCCGGACCACCAGGAACTCCGTCGCCTGGGTAATGAGGTATTGAATGTGTTGCGCCAACACCCCAACGTCA
AAGGAATTCGCAACGAATGGGGCGAGCAGGTGCAGGTCATCAAGCCGGTCATGTCGGATGTGCTGGCCAGCCAGCTGGGC
ATCGGCCGACCTGAAATCGCGCAGGCAACTGCACACACCGTCGAAGGTGTCCGTGCGGGCGTGTATCGGGAGCGCGACGA
GTTAATTCCCATTGTCGCGCGTGCACCTGAACATGAACGCCGGAACCTGGACAATCTCGATCAGCTCCCGATCTGGAGCC
CGACCGCCCAACGCATGATTCCACTGGGTCAGGTCGTGAGCCACTTCGATGTGGATTTTGAAAATCCGCACATCTGGCGT
CAGGATCGGGTCAGCATGCTGCGCATTCACTTCGATCAACGTGAAGGCTTGTCCTCAGAGCTGTTGGCTGACCTCAAAGC
CGACGTGGAAAAAGCGATCAATGTCGATGTTGCGGGCTATCTCAAACTCGCCGCCGATGCAGACATCGATCACCAGTCAT
CCACCATCCCGATCAAATACCGCGATCGGCTACCCATTGCCGGCAAGCCGGGCTATTACGCCGCCTGGGGCGGCGAGAAC
GAAGATTCGGATAAAGGCAGTACCGCCATAATGGGCTCGATTCCCGTGTTCTTTGGATTGATGGTATTTATCGTGATCTG
TCTGTTCAATTCATTGCGCAAAACGGCGGTGATCTGGCTGGTGGTACCCTTGGCGATCGTGGGCGTGACCGCCGGCTTAC
TGCTGTTCAACCAGCCCTTCGGCTTCATGGCGTTGCTGGGTTTCATGAGCCTGGCTGGCATGCTGATCAAGAACGCCATT
GTGCTGGTAGATCAGATTGGAGAGGAATTGAGCACCGGTAAAAACGGCGTAGAGGCGGTGATTGAATCCGGTGTCAGCCG
GCTGATTCCCGTGGCCATGGCGGCGGCCACCACCATCCTGGGTATGATCCCACTGCTGGGGGATGCATTTTTCATCGCCA
TGGCTGTGACCATCATGTTTGGTCTGGGCTTCGCGACAGTGCTGACCCTGGTGGTCGTACCGGTGCTTTACGCCACCATT
TTCAGGTTCAGATAA

Protein sequence :
MNIAERSIKNSTITWVVTGILLVVGMIAFNNLSRLEDPEFTIKEAMIITPYPGASAREVEIEVSDLVERATQEMGQLFYV
ESHSYRDKSIVKVRIKDQYDKDGLPQVWDELRRKVNDIQHQLPPGAGPSLVNDDFGDVFGVYIALTGDGYTQKDLYEFAK
FLRRELLLVQDVKKITFYGQPKEVIYVEMQRDKMTALGISPQDIYSALGAKNTAEQAGYMTVGNRRLAVSPTGEFHSEQQ
FAGLVINARNLQNQQVVLGDVATITRGLQDPPNNLLRFDGEPAIGLAISTTSGGNVVTMGEGLYQKVEELKPQIPLGIQL
NVVSMQSDSVVEAINNFLISLIEAVVIVVVVLLFFMGLRSGLIIGTVLAVTITGTFIFMSILGITLERISLGALIIALGM
LVDNAIVVTDGMRVRMEQGEDALSAARKVVSQTAMPLLGATAVAIAAFAAIGTSQDATGEYTRSLFTVILISLTMSWVTA
VTTTPLLCKTFLKSGQLGSQGAPYQGVFYQRYRQFLTLAIQHRWVAIGITVALFAASLIGFGYVKQSFFPDSTRPQFMVD
LWLPEGTHIHETERRLAPFEEKLKGYSNVTKVTTQVGGSSPRFLLTYQPEQPNSNFARLLVDVDDYRIINQFSQQVQNDL
EFIDPDVNVNVRLFVLGPATGGKVQLRLSGPDHQELRRLGNEVLNVLRQHPNVKGIRNEWGEQVQVIKPVMSDVLASQLG
IGRPEIAQATAHTVEGVRAGVYRERDELIPIVARAPEHERRNLDNLDQLPIWSPTAQRMIPLGQVVSHFDVDFENPHIWR
QDRVSMLRIHFDQREGLSSELLADLKADVEKAINVDVAGYLKLAADADIDHQSSTIPIKYRDRLPIAGKPGYYAAWGGEN
EDSDKGSTAIMGSIPVFFGLMVFIVICLFNSLRKTAVIWLVVPLAIVGVTAGLLLFNQPFGFMALLGFMSLAGMLIKNAI
VLVDQIGEELSTGKNGVEAVIESGVSRLIPVAMAAATTILGMIPLLGDAFFIAMAVTIMFGLGFATVLTLVVVPVLYATI
FRFR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC0395_A1355 YP_001217299.1 AcrB/AcrD/AcrF family transporter Not tested VPI-2 Protein 1e-179 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M5M_08260 YP_006916571.1 acriflavin resistance protein BAC0428 Protein 0.0 44
M5M_08260 YP_006916571.1 acriflavin resistance protein BAC0425 Protein 6e-180 42
M5M_08260 YP_006916571.1 acriflavin resistance protein BAC0519 Protein 0.0 42
M5M_08260 YP_006916571.1 acriflavin resistance protein BAC0526 Protein 1e-169 41