Gene Information

Name : MC28_0390 (MC28_0390)
Accession : YP_006827211.1
Strain : Bacillus thuringiensis MC28
Genome accession: NC_018693
Putative virulence/resistance : Virulence
Product : Zn-dependent protease
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 372513 - 375113 bp
Length : 2601 bp
Strand : +
Note : -

DNA sequence :
ATGGACTTAAATCAAATGACAACGAAAACACAAGAGGCGATTATGAGTGCCCAATCTTTAGCGGTATCTCATCATCACCA
AGAGGTGGATACTGTTCATCTATTACTTGCATTATTAGAGGAGCAAGATGGATTAGCAGTACATATATTTCAAAAAATGA
ATGTTGATATAGAAGCATTGAAGCAAGATGTAGAAAATGTAATTAAAAAGAAACCTTCTGTAACGGGAAGCGGAGCAGAA
GCTGGGAAATTGTATGTAACAAGCAATCTGCAGCAACTGCTTGTAAGAGCAGGGAAAGAAGCAGAAAAACTGCAGGATGA
TTACATTTCAGTAGAACATGTATTGCTTGCTTTTTCTGAAGAAAAAGGCGATATCAATCATTTATTTACAAGATTGCATA
TTACGAAAGATAACTTATTACAGTCTTTAATGACAGTTCGGGGGAATCAAAGAGTGACTAGTCAAAATCCAGAAGCAACT
TATGAAGCATTAGAAAAATATGGCCGTGATTTAGTGGCGGAAGTGAGAGCAGGGAAAATTGATCCTGTTATCGGACGAGA
TAGTGAAATTCGCCGCGTTATTCGCATTCTTTCACGTAAAACGAAAAACAACCCTGTTTTAATTGGTGAGCCAGGTGTTG
GTAAAACAGCAATTGTTGAAGGATTAGCACAGCGTATCGTGAAAAAAGATGTACCAGAAGGGTTAAAAGATAGAACAATC
TTTGCATTAGATATGAGTGCACTTGTAGCCGGAGCAAAATTCCGTGGTGAATTTGAAGAGCGCCTGCAAGCTGTATTAAA
TGAAATTAAAAAGAGTGAAGGCCGTATTTTATTATTCATTGATGAACTTCATACAATCGTCGGCGCTGGTAAAACAGAAG
GTGCGATGGACGCAGGAAATATGTTAAAACCGATGCTTGCTCGTGGTGAACTGCATTGTATCGGGGCAACGACACTAGAT
GAATATCGTAAATATATTGAGAAAGACCCAGCGTTAGAAAGACGTTTCCAACAAGTATTAGCAGAGGAACCGACTGTTGA
AGATACAATCTCCATTTTACGCGGTTTAAAAGAGCGTTTTGAAATTTATCACGGTGTAAACATTCATGACCGCGCGATTG
TAGCAGCATCTGTTTTATCAGATCGATATATTTCAGATCGCTTCTTACCGGATAAAGCAATTGACCTTGTTGACGAAGCA
TGCGCAACAATTCGTACAGAAATTGATTCTATGCCAACAGAATTAGATGAAGTAACGCGCCGTATTATGCAGCTGGAAAT
TGAAGAAGCCGCTCTTGGAAAAGAGAAGGACTTTGGTAGCCAAGAACGTCTAAAAACATTGCAACGTGAATTATCGGATT
TAAAAGAAGTTGCAAGTAGCATGAGAGCGAAATGGGAGAAAGAAAAAGAAGATATTCACAAAGTTCGTGACTTACGTGAA
CATTTAGAGCGTCTGCGCCGTGAATTAGAAGAAGCAGAAGGTAATTACGATTTAAATAAAGCAGCTGAACTTCGTCACGG
AAAAATTCCTGCAATTGAAAAAGAATTAAAAGAAGCGGAAGAAATGAGCGCACATAATAAACAAGAAAATCGTTTATTAC
GCGAGGAAGTCAGTGAAGAAGAAATTGCTGATATCGTTTCACGCTGGACTGGTATTCCTGTCGCAAAACTCGTTGAGGGT
GAACGTGAGAAATTACTACGCTTAGAGCAAATTTTATCAGAGCGTGTCATCGGACAAGAGGAAGCGGTAAGTTTAGTATC
AGACGCAGTTCTTCGTGCACGCGCTGGTATTAAAGACCCGAACCGTCCAATTGGTTCCTTCATTTTCTTAGGACCGACAG
GTGTTGGTAAAACAGAACTTGCAAAAACGTTAGCGCAGTCTTTATTCGATAGTGAAGAGCAAATGATTCGTATCGACATG
TCTGAGTATATGGAGAAACACGCTGTGTCACGCTTAATTGGTGCGCCTCCTGGATATGTTGGATATGAAGAGGGCGGTCA
ATTAACAGAGGCGGTAAGACGTAAACCATATTCCGTTATTTTGTTAGACGAAATCGAAAAAGCACATCCAGAAGTATTCA
ACATTTTATTACAAATGTTAGATGATGGACGCATTACAGATTCGCAAGGACGTACAGTAGACTTTAAAAACACGGTTATT
ATTATGACTTCAAATATTGGATCTGCTCATTTGTTAGATGGATTAGAAGCAGATGGCTCCATTAAAGAGGAATCAAGAGA
ACTTGTAATGGGGCAATTAAGAGGACATTTCCGACCAGAGTTCTTAAACCGTGTCGATGAAATTATTTTATTCAAACCGC
TTACAACGAATGAAATTAAAGGTATTGTTGATAAAATTGTAAAAGAATTACAAGGTCGTTTAGCTGACCGTCATATTACA
GTGGAATTAACGGATTCTGCAAAAGAATTTGTTGTAGAAGCTGGCTTTGATCCGATGTACGGAGCTCGTCCATTAAAACG
ATACGTACAGCGTCAAGTGGAAACGAAATTAGCGAGAGAATTAATTGCAGGCACAATTACGGATAATAGTCACGTAGTTG
TTGATGTAGAAAATAACGAATTAGTTGTTCATGTGAAATGA

Protein sequence :
MDLNQMTTKTQEAIMSAQSLAVSHHHQEVDTVHLLLALLEEQDGLAVHIFQKMNVDIEALKQDVENVIKKKPSVTGSGAE
AGKLYVTSNLQQLLVRAGKEAEKLQDDYISVEHVLLAFSEEKGDINHLFTRLHITKDNLLQSLMTVRGNQRVTSQNPEAT
YEALEKYGRDLVAEVRAGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKKDVPEGLKDRTI
FALDMSALVAGAKFRGEFEERLQAVLNEIKKSEGRILLFIDELHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLD
EYRKYIEKDPALERRFQQVLAEEPTVEDTISILRGLKERFEIYHGVNIHDRAIVAASVLSDRYISDRFLPDKAIDLVDEA
CATIRTEIDSMPTELDEVTRRIMQLEIEEAALGKEKDFGSQERLKTLQRELSDLKEVASSMRAKWEKEKEDIHKVRDLRE
HLERLRRELEEAEGNYDLNKAAELRHGKIPAIEKELKEAEEMSAHNKQENRLLREEVSEEEIADIVSRWTGIPVAKLVEG
EREKLLRLEQILSERVIGQEEAVSLVSDAVLRARAGIKDPNRPIGSFIFLGPTGVGKTELAKTLAQSLFDSEEQMIRIDM
SEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILLDEIEKAHPEVFNILLQMLDDGRITDSQGRTVDFKNTVI
IMTSNIGSAHLLDGLEADGSIKEESRELVMGQLRGHFRPEFLNRVDEIILFKPLTTNEIKGIVDKIVKELQGRLADRHIT
VELTDSAKEFVVEAGFDPMYGARPLKRYVQRQVETKLARELIAGTITDNSHVVVDVENNELVVHVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY0294 NP_454876.1 ClpB-like protein Not tested SPI-6 Protein 3e-107 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
MC28_0390 YP_006827211.1 Zn-dependent protease VFG0079 Protein 1e-180 49
MC28_0390 YP_006827211.1 Zn-dependent protease VFG2084 Protein 5e-110 42