Gene Information

Name : O3K_04220 (O3K_04220)
Accession : YP_006777574.1
Strain : Escherichia coli 2011C-3493
Genome accession: NC_018658
Putative virulence/resistance : Virulence
Product : antigen 43 (Ag43) phase-variable biofilm formation autotransporter, CP4-44 prophage
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 864916 - 868035 bp
Length : 3120 bp
Strand : -
Note : COG2931 RTX toxins and related Ca2+-binding proteins

DNA sequence :
ATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATTACGGGCGCTTTCGTGGTTGCCTCCGAACTGGC
CCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTG
ACATCGTTGTGCACCCGGGTGAAACAGTGAATGGCGGAACACTGGTAAACCATGACAACCAGTTTGTATCCGGAACAGCT
GATGGCGTGACTGTCAGTACCGGGCTTGAGCTGGGGCCGGACTCTGATGACAACACCGGCGGTCAGCAGATAGCCCGGGG
TGGCACTGCCAGAAACACCACGGTCACCGCAAACGGTCTGCAGGATGTCATGGCAGGTGGCAGTGCCACGGATACGGTTA
TCAGTGCCGGTGGAGGACAGAATCTGCGCGGGCAGGCTTACGGTACTGTCCTTAACGGTGGTGAACAATGGATACATGCA
GGCGGCAGTGCATCCGGAACGGTGATTAACCAGTCTGGCTACCAGACCATTAAACATGGCGGGCAGGCAACCGGAACCAT
CGTCAACACCGGTGCAGAAGGTGGTCCGGAGTCTGAAAATGTGTCCAGCGGTCAGATGGTCGGAGGGACGGCTGAATCCA
CCACCATCAACAAAAATGGCCGGCAGGTTATCTGGTCTTCGGGGATGGCACGGGACACCCTCATTTACGCCGGCGGTGAC
CAGACGGTACACGGCGAGGCACATAACACCCGGCTGGAGGGAGGAAACCAGTATGTGCACAAATACGGACTTGCGCTGAA
CACGGTAATTAACGAGGGGGGCTGGCAGGTGATTAAGGAAGGAGGAACTACCGCGCATACCACCATCAACCAGAAAGGAA
AGCTGCAGGTGAATGCCGGCGGTAAAGCGTCTGATGTCACCCAGAACACGGGAGGTGCACTGGTTACCAGCACAGCTGCA
ACCGTCACCGGCACAAACCGCCTGGGAGCATTCTCCGTTCTGGCGGGTAAGGCTGATAATGTCGTACTGGAAAATGGCGG
ACGTCTGGATGTGCTGAGCGGACACACAGCCACTAATACCCGTGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTG
GTGCCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGGGCCGCTGTCAGTGGTACCCGGAGC
GACGGAACGGCATTCCGTATCGGGGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGC
CGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGTTCACTGCCAGAGGGGGCTCGCTGGCGGGCACCACCACGC
TGAATAACGGTGCCACGCTTACCCTTTCCGGGAAAACGGTGAATAACGATACCCTGACCATCCGTGAAGGTGATGCACTC
CTGCAGGGAGGCGCTCTTACCGGTAACGGCAGGGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACT
CACCCAGAAAACCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATCGCTCAGC
GCGGCACGGCCCTGAAGCTGACCGGCAGCACAGTGCTGAACGGTGCCATTGACCCCACGAATGTCACCCTGACATCCGGT
GCCACCTGGAATATCCCTGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCAC
CTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTCC
GTGTATGCCCGGATATGGCGCAGAACAATGCTGACAGACTGGTCATTGACGGTGGCAGGGCAACCGGAAAAACCATCCTG
AATCTGGTGAACGCCGGCAACAGTGGTACGGGGCTGGCGACCACCGGTAAGGGTATTCAGGTGGTTGAGGCCATTAACGG
TGCCACCACGGAGGAAGGGGCTTTTGTCCAGGGGAATATGCTGCAGGCCGGGGCCTTTAACTACACCCTCAACCGGGACA
GTGATGAGAGCTGGTATCTGCGCAGTGAAGAACGTTATCGTGCTGAAGTCCCCCTGTACGCCTCCATGCTGACACAGGCA
ATGGACTATGACCGGATTCTGGCAGGCTCACGCAGCCATCAGACCGGTGTAAACGGTGAAAATAACAGCGTCCGTCTCAG
CATTCAGGGCGGCCATCTCGGTCACGATAACAACGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCAGCTATG
GCTTCGTCCGTCTGGAGGGTGACCTGCTCAGAACAGAGGTCGCCGGTATGTCACTGACGACAGGAGTGTATGGTGCTGCA
GGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGCGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGG
ATATATGAACCTGACACACACCTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGTATGAAAGCGT
CATCGGGCAATAACGACTTCCGCGCACGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATTACT
GACAACCTGATGCTGGAGCCACGGCTGCAGTACACCTGGCAGGGGCTTTCGCTGGATGACGGTAAGGACAACGCCGGTTA
TGTGAAGTTCGGGCATGGCAGTGCACAACATGTGCGTGCCGGTTTCCGTCTGGGCAGCCACAACGATATGACCTTTGGTG
AAGGCACCTCATCCCGTGCCCCCCTGCGTGACAGTGCAAAACACAGTGTGCGTGAACTGCCGGTGAACTGGTGGGTACAG
CCTTCCGTTATCCGCACCTTCAGCTCCCGGGGTGATATGCGTGTGGGGACCTCCACCGCAGGCAGCGGGATGACGTTCTC
TCCCTCACAGAATGGCACTTCACTGGATCTGCAGGCCGGACTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTC
AGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACGCTGAATGTGACCTTCTGA

Protein sequence :
MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTA
DGVTVSTGLELGPDSDDNTGGQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWIHA
GGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGD
QTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQVIKEGGTTAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAA
TVTGTNRLGAFSVLAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSGAAVSGTRS
DGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIREGDAL
LQGGALTGNGRVEKSGSGTLTVSNTTLTQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSG
ATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLQVKNLNGQNGTISLRVCPDMAQNNADRLVIDGGRATGKTIL
NLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQA
MDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAA
GHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSIT
DNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQ
PSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNSQATLNVTF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66200.1 hypothetical protein Virulence PAI III 536 Protein 0.0 93
sap AAK00474.1 Sap Not tested SHI-1 Protein 0.0 92
SF2991 NP_708765.1 outer membrane fluffing protein Not tested SHI-1 Protein 0.0 92
aidA ADD91708.1 AidA Not tested PAI-I AL862 Protein 0.0 87
flu CAI43838.1 antigen 43 Not tested LEE Protein 0.0 87
Z1211 NP_286746.1 adhesin Not tested TAI Protein 0.0 58
Z1651 NP_287154.1 adhesin Not tested TAI Protein 0.0 58
unnamed AAL08472.1 putative autotransporter Not tested SRL Protein 0.0 57
unnamed CAE85197.1 antigen43 protein orthologue Not tested PAI V 536 Protein 0.0 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
O3K_04220 YP_006777574.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter, CP4-44 prophage VFG1675 Protein 0.0 93
O3K_04220 YP_006777574.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter, CP4-44 prophage VFG0655 Protein 0.0 92
O3K_04220 YP_006777574.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter, CP4-44 prophage VFG1063 Protein 0.0 57