Gene Information

Name : BTF1_09035 (BTF1_09035)
Accession : YP_006609936.1
Strain : Bacillus thuringiensis HD-789
Genome accession: NC_018508
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 1754917 - 1756533 bp
Length : 1617 bp
Strand : +
Note : COG1021 Peptide arylation enzymes

DNA sequence : 
ATGCTAGTAGGTTATAGGGAATGGCCAAAAGAATTTGCCGATCGTTATCGAGAAGAAGGGTGTTGGCTTGGTGAAACATT
TGGAGGAGTGTTAAGAGAGCGAGCTGAGAAATATGGAGATCAAATCGCGGTTGTAAGCGGTAAGACGCATGTAACGTACA
GTGAACTTGATAAAAAGGTAGATCGTTTAGCAGCAGGTTTACTGAATTTAGGAATAAAGAAAGAGGACAGAGTTGTAATA
CAGTTACCTAACATTATCGAGTTTTTCGAAATATGTTTTGCACTATTTCGAATTGGAGCGCTTCCTGTCTTTGCACTGCC
TTCACATCGAAGTAGTGAAATTAGTTGTTTTTGTGAGTTTGGTGAGGCGAGCGCTTACGTTATTTCAGATAAGGCCCTCG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTGCCAACTTTACAACATGTAATTGTAGTGGGAGAAGAG
GAAGAGTTTGTGAACATAAATGATCTGTATATGGATCCTATCTCATTGCCAGAAGTTCAGCCCAGTGATGTTGCATTTCT
CCAATTATCAGGAGGGACAACAGGTCTTTCTAAATTAATTCCTAGAACACATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTGAATGCAGAAAGCGTCTATATGACAGTTCTTCCAGTAGCACACAATTATCCAATGAGTTCT
CCAGGAACATTTGGAACTTTCTATGCGGGTGGAAAAGTGGTATTGGCAACTGGTGGTAGCCCAGATGAGGCATTTGCTCT
TATCGAAGAAGAAAAAGTTACAATTACAGCGCTCGTTCCGCCATTAGCAATGATTTGGCTTGATGCTGCATCTTCTCGTA
ATGCCGATTTATCGAGCCTAGAGGTTATTCAAGTAGGTGGTGCTAAGTTTAGTGCTGAGGTTGCAAAACGTATACGCCCT
ACATTTGGATGTACGTTACAGCAAGTATTCGGTATGGCGGAAGGATTAGTGAACTATACCAGATTAGACGATCCGGAAGA
AATTATTATTCATACACAAGGTAGACCAATGTCTGCACACGATGAAGTACGAGTTGTTGATGAAAATGACAACGATGTAA
AACCTGGCGAAGTAGGTAGTTTATTAACACGAGGTCCATATACAATTCGTGGTTACTATAAAGCCGAAGAGCATAATGCA
CGATCATTTACAAAGGATGGATTTTATCGCACAGGAGATCTTGTAAAAGTAAATGAACAAGGATACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAACCGTGGTGGTGAGAAAGTTGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACACGATG
CGGTTCATGACGTAGCAATTGTATCTATGCCTGACGATTATTTAGGTGAACGCACCTGTGCTTTTGTTATAGCTCGCGGA
CAAGTTCCAACTGTAAGTGAATTAAAAATGTTTTTAAGAGAACGTGGTATAGCGGCTTATAAAATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGAGTCGGAAAAGTCAGCAAAAAAGAACTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLVGYREWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGKTHVTYSELDKKVDRLAAGLLNLGIKKEDRVVI
QLPNIIEFFEICFALFRIGALPVFALPSHRSSEISCFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNINDLYMDPISLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLNAESVYMTVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEEEKVTITALVPPLAMIWLDAASSRNADLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSAHDEVRVVDENDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QVPTVSELKMFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-119 51
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 51
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 9e-119 51
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 51
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-119 51
ybtE CAA21394.1 - Virulence HPI Protein 9e-119 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-118 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-119 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-119 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BTF1_09035 YP_006609936.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-136 53
BTF1_09035 YP_006609936.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 5e-112 47
BTF1_09035 YP_006609936.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-109 45