Gene Information

Name : BCK_23065 (BCK_23065)
Accession : YP_006598693.1
Strain : Bacillus cereus FRI-35
Genome accession: NC_018491
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 4508975 - 4510591 bp
Length : 1617 bp
Strand : -
Note : COG1021 Peptide arylation enzymes

DNA sequence : 
ATGCTAACAGGTTATACAAAATGGCCAAAAGAATTTGCTGATCGTTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGTGATGTTAAGAGAGCGAGCTGAAAAATATGGCGATCAAATTGCAGTTGTAAGTGGTAATACGCATATTACGTATA
GTGAGCTTGATAAAAAAGTAGATCGATTAGCTTCAGGGCTACTGAATTTAGGAATAAAGCAAGAGGATCGAGTTGTAATC
CAGCTACCTAACGTTATTGAGTTTTTCGAAGTATGTTTTGCGTTATTTCGAATTGGGGCACTTCCTGTTTTTGCATTACC
TTCACATCGAAGCAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCTTACGTTATTTCAGATAAAACTCTCG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTGCCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAATTTGTGAACATAAGTGATTTATATATAGATCCCGTTCCATTACCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGGGGGACAACAGGACTTTCTAAATTAATTCCTCGAACGCATGATGATTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAAAGAGTGTCTATATGGCCGTTCTTCCAGTAGCACATAATTACCCGATGAGCTCT
CCTGGAACATTTGGAACTTTCTATGCGGGTGGAAAAGTAGTGCTGGCAACTGGAGGTAGTCCAGATGAGGCGTTTGCACT
TATTGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGATGCTGCATCTTCACGTA
ATAACGATTTATCGAGTCTAGAGGTGATTCAAGTAGGTGGCGCGAAATTTAGTGCTGAAGTTGCAAAACGTATACGCCCT
ACATTTGGATGTACATTACAGCAAGTATTCGGTATGGCGGAAGGATTAGTGAACTATACAAGATTAGATGATCCTGAAGA
AATTATTATTCATACACAAGGTAGACCAATGTCTGCACTCGATGAAGTACGAGTTGTTGATGAAAATGACAACGATGTAA
AACCTGGCGAAGTAGGTAGTTTATTAACAAGAGGGCCATATACAATTCGTGGTTATTATAAAGCAGAAGAGCATAATGCA
CAATCGTTTACAAAGGATGGATTTTATCGCACAGGAGATCTTGTGAAAGTAAATGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTTGCCGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCGATTGTATCTATGCCTGACGATTATTTAGGAGAACGTACTTGTGCATTTGTTATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAATGTTTTTAAGAGAACGCGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGCGTTGGAAAGATAAGCAAAAAAGAATTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLTGYTKWPKEFADRYREEGCWLGETFGVMLRERAEKYGDQIAVVSGNTHITYSELDKKVDRLASGLLNLGIKQEDRVVI
QLPNVIEFFEVCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKTLGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAKSVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDENDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
QSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKMFLRERGIAAYKIPDRIEFIESFPQTGVGKISKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-120 51
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-120 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-120 51
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-120 51
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-120 51
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-120 51
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-120 51
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-120 51
ybtE CAA21394.1 - Virulence HPI Protein 7e-120 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BCK_23065 YP_006598693.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-136 53
BCK_23065 YP_006598693.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 6e-112 47
BCK_23065 YP_006598693.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-111 45