Gene Information

Name : ECENHK_14420 (ECENHK_14420)
Accession : YP_006579355.1
Strain : Enterobacter cloacae ENHKU01
Genome accession: NC_018405
Putative virulence/resistance : Resistance
Product : acriflavin resistance protein
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 3051800 - 3054862 bp
Length : 3063 bp
Strand : +
Note : COG0841 Cation/multidrug efflux pump

DNA sequence :
ATGGATATCTCTCGCCAGTTTATTAATAACCCCATTCGCGTCTGGCTAACGATCCTGCTGCTCGGCGTGGGGGGCATTTT
CGCCCTGCTGAACATTGGCAGACTGGAAGATCCGGCCTTTACCATCAAAACGGCCGTGGTCATTACCCACTATCCTGGGG
CCTCTGCTCAGCAGGTGGAAGAGGAGGTCACGCTGCCCCTCGAGAATGCCCTCCAGCAGCTGCCCTATCTGGACAATGTC
AGCTCTATCTCATCAAACGGCTTGTCGCAAATCACCGTGAACATTGCCTCGCGCTATCATTCGAACGAACTGCCGCAGAT
CTGGGACGAACTGCGCCGTCGCGTGGGCGATGCCGCGCGCCAGTTCCCACCCGGCGTCGTGACGCCCTTTGTAAATGATG
ATTTTGGCGATGTCTTCGGCTTTTTCTTCGCGATTTCCGGGGATGAATTCAGCAATCCTGAACTGGTGAGGTATGCCGAG
CAGCTGCGTCGTGAACTGATTCTGGTCCCCGGCGTCGGTAAAGTTGCCATCGGCGGGGCCATCAGCCAGCAGGTCAACAT
CGATATTTCCCTGACCAAAATGGCCGCGCGCGGCATCACGCTGAACCAGCTTTCCGCCCTGCTCGGCAGGCTGAACGTGG
TTTCCAGCGCCGGAGAAATCACCTCGGGAACGGAATCTATTCGCCTGCATCCAACCGGTGAGTTTGAAAATCTGGATGAA
CTGGCCGCTCTCATCATCACCCCTCCCGGCATCGGGGCAGCGACGCGTCTACGGGATATCGCCACGCTGTCGCGCGGGCT
GAACGAATCGCCCGCCAGTATCTACCATGCTAACGGCAGAAAAGCCGTCACGATGGGCGTCTCGTTTATTCCCGGCGTGA
ACGTGATCGACGTGGGACACGCGCTGGAGGCGAAGCTAAACCAGATGTCAGCGGAAAAACCGGCGGGGATACACATCGAC
CTGTTCTACGATCAGGCCGCCGAAGTGGGCCACTCTGTTAACGGTTTTATCATTAACTTTCTGATGGCGCTGGCGATTGT
CATCGGCGTGCTGCTGATCTTTATGGGCGTACGCAGCGGGATTATCATCGCGTTTTCCCTCGCGCTTAACGTGCTGGGCA
CGCTGCTGATCATGTATCTGTGGGGCATTGAGCTGCAGCGTATCTCGCTCGGCGCGCTGATCATCGCCCTCAGCATGCTG
GTGGATAACGCCATCGTGATAGTTGAAGGGGTACTGATTGCGCGTCAGCAGGGCTCGCCACTATTGACCGCCATTAGCTA
CATCATCCGTCGCTCCGCCCTGCCGCTGCTGGGGGCAACGGTGATCGCCATCCTGGCTTTTGCGCCTATCGGGCTGTCGC
AGGATTCTACCGGGGAATATTGTAAATCCCTGTTCCAGGTGCTGCTGATTTCCCTGATGCTGAGCTGGTTCTCTGCGCTT
ACCATCACGCCGGTGCTGATTAAGTGGTGGTTGTTTAAAAACGACGGCGCGGCTGTGAAACCCGACGAGTCAAATCCTTA
CGACAAACGCCTCTACCGCCTTTATCAGCGTCTCCTGAACGCGCTGCTGTTCCGGAAAGCGCCGACACTCATCGTTATGG
CTGCGCTGCTGGTCGCGTCAGTCTGGGGTTTTGGCGCCGTGCGGCAAAACTTTTTCCCCTCGTCTAATACGCCGATTTTC
TTTGTCGACCTGTGGTTACCGTATGGCACCGATATCAAATGGACCGAGAAGATGACCAGTGATATCGAAAAAACCATCAA
CGGCCAGCCCGGCGTGGAAACCACCGTTTCAACCATCGGTCAGGGCAGCATGCGGTTTATTCTGACCTACAGCGGACAGC
GGCAGTACAGCAACTACGCGCAGATCATGGTGCGCATGGATGACCAGCGCAACATCTCCGCATTGACGCGTCACGTCGAT
GAGTACATTGCGCGAAACTATCCGCAGGTTAACGCCAGCACCAAACGCGTGATGTTTGGCCCCTCCGGGGACAGCGCCAT
TGAGGTGCGCATCAAGGGTCCCGATCCCGACAGGCTGCGCCTGATTGCCAGCCAGGTGGACGATATTCTGGCGCGCGACC
CGGCGACAAGCAGCGTAAGAAACGACTGGCAAAACCGCAGCAAGGTGATCCGTCCGCAGTACGTCGCCGCGCTTGGACGC
GAGCTTGGCGTGGACAAACAGGACGTCGACAACGCGCTGGAGATGAATTTTTCCGGCAGCCGGGCAGGATTATATCGGGA
AGGCAGCGACCTGCTCCCTGTCGTGGTGCGCCCGCCGGAGAGCGAACGGCTGGACGCCAATCACCTGAACAACGTGCTTG
TCTGGAGCCAGACGCGGCAGCAGTATATCCCGCTAAGTAACGTTGTTAGCCGTTTCTCGCTGGAGTGGGAAGATCCGCTT
ATTCTGCGCCGCGACCGCTCGCGGGTGCTGACGGTTATGACCGACCCCGATCCCCTCAGCCAGCAAACCTCCGGCGATAT
TCTCGCCCGGGTGAAGCCGCACATTGACGCCCTTCCTCTGCCCCACGGCTACAGCATCGAGTGGGGAGGCGACGCAGAAA
ACTCCAGCGAAGCCCAGCAGGGGCTCTTCACCACGCTGCCGATCGGATTCCTGGTCATGTTTGTTATCACCGTGTTGATG
TTCAGCTCGGTGAAAAACGCCGTCGCCATCTGGCTGACCGTGCCGCTGGCGCTGATTGGCGTTACGCCGGGATTCTTAAT
TACCGGGATCCCGTTCGGCTTTATGGCGCTGATTGGTCTCCTGAGCCTGAGCGGAATGCTCATTCGCAACGGCATCGTGC
TGGTGGAAGAGATCGAGCAGCAGAAGGCGCATAAAGGTCAGCACGAGGCGATCGTTTACGCCGCCACCTCACGTCTGCGG
CCTATCCTGCTTACCGCATTTACGACCGTACTGGGCCTGGCGCCGCTGCTGCTGGATGTTTTCTTCCAGAGCATGGCCGT
TGTGATTATGTTTGGACTGGGGTTTGCGACTATCCTGACGCTGCTGGTTCTTCCTGTAATATATGCATGTTTCCATCGTA
AGGACGAAGCCAGACAACAATGA

Protein sequence :
MDISRQFINNPIRVWLTILLLGVGGIFALLNIGRLEDPAFTIKTAVVITHYPGASAQQVEEEVTLPLENALQQLPYLDNV
SSISSNGLSQITVNIASRYHSNELPQIWDELRRRVGDAARQFPPGVVTPFVNDDFGDVFGFFFAISGDEFSNPELVRYAE
QLRRELILVPGVGKVAIGGAISQQVNIDISLTKMAARGITLNQLSALLGRLNVVSSAGEITSGTESIRLHPTGEFENLDE
LAALIITPPGIGAATRLRDIATLSRGLNESPASIYHANGRKAVTMGVSFIPGVNVIDVGHALEAKLNQMSAEKPAGIHID
LFYDQAAEVGHSVNGFIINFLMALAIVIGVLLIFMGVRSGIIIAFSLALNVLGTLLIMYLWGIELQRISLGALIIALSML
VDNAIVIVEGVLIARQQGSPLLTAISYIIRRSALPLLGATVIAILAFAPIGLSQDSTGEYCKSLFQVLLISLMLSWFSAL
TITPVLIKWWLFKNDGAAVKPDESNPYDKRLYRLYQRLLNALLFRKAPTLIVMAALLVASVWGFGAVRQNFFPSSNTPIF
FVDLWLPYGTDIKWTEKMTSDIEKTINGQPGVETTVSTIGQGSMRFILTYSGQRQYSNYAQIMVRMDDQRNISALTRHVD
EYIARNYPQVNASTKRVMFGPSGDSAIEVRIKGPDPDRLRLIASQVDDILARDPATSSVRNDWQNRSKVIRPQYVAALGR
ELGVDKQDVDNALEMNFSGSRAGLYREGSDLLPVVVRPPESERLDANHLNNVLVWSQTRQQYIPLSNVVSRFSLEWEDPL
ILRRDRSRVLTVMTDPDPLSQQTSGDILARVKPHIDALPLPHGYSIEWGGDAENSSEAQQGLFTTLPIGFLVMFVITVLM
FSSVKNAVAIWLTVPLALIGVTPGFLITGIPFGFMALIGLLSLSGMLIRNGIVLVEEIEQQKAHKGQHEAIVYAATSRLR
PILLTAFTTVLGLAPLLLDVFFQSMAVVIMFGLGFATILTLLVLPVIYACFHRKDEARQQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC0395_A1355 YP_001217299.1 AcrB/AcrD/AcrF family transporter Not tested VPI-2 Protein 4e-172 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ECENHK_14420 YP_006579355.1 acriflavin resistance protein BAC0519 Protein 0.0 50
ECENHK_14420 YP_006579355.1 acriflavin resistance protein BAC0428 Protein 0.0 50
ECENHK_14420 YP_006579355.1 acriflavin resistance protein BAC0526 Protein 0.0 48
ECENHK_14420 YP_006579355.1 acriflavin resistance protein BAC0425 Protein 2e-172 41