Gene Information

Name : fliI (ECENHK_14100)
Accession : YP_006579293.1
Strain : Enterobacter cloacae ENHKU01
Genome accession: NC_018405
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 2984131 - 2985501 bp
Length : 1371 bp
Strand : +
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGACCGCACGCCTCACCCGCTGGCTCAACACGCTCGACAACTTTGAGACGAAGATGGCGCAGCTGCCATCCGTTCGTCG
TTATGGACGGCTGACGCGCGCGACCGGTCTGGTACTGGAAGCCACCGGCCTGCAGCTTCCGCTGGGCGCAACCTGTGTGA
TTGAGCGTCAGGATGGTCAGGAAACGCGTGAAGTCGAAAGCGAAGTGGTTGGGTTTAACGGTCAGCGACTGTTCCTGATG
CCGCTTGAAGAGGTGGAAGGTATTCTCCCCGGCGCCCGCGTCTACGCGAAAAACATCAGCGGCGACGGCCTGCAAAGCGG
GAAACAGCTGCCGCTTGGTCCGGCCCTGCTCGGCCGCGTGCTCGACGGCAGCGGGAAACCGCTGGATGGCCTGCCTGCGC
CGGACACCACCGAAACCGGGGCGTTGATCACCCAGCCGTTTAACCCCCTGCAGCGTACCCCTATCGAGCATGTGCTGGAT
ACCGGCGTACGGCCGATTAACGCCCTGCTCACGGTAGGACGCGGACAACGTATGGGTCTGTTCGCCGGCTCCGGCGTGGG
TAAATCGGTCCTGCTCGGCATGATGGCGCGTTATACCCAGGCCGACGTGATCGTCGTTGGGTTGATCGGTGAGCGTGGCC
GTGAAGTCAAAGATTTCATCGAAAACATTCTGGGTGCGGAAGGCCGGGCCCGTTCAGTGGTAATCGCCGCCCCGGCGGAT
GTGTCCCCCCTGCTGCGTATGCAGGGTGCCGCCTACGCCACTCGTATTGCCGAAGATTTCCGCGACCGCGGCAAGCACGT
GCTGCTGATCATGGACTCCCTCACCCGCTACGCGATGGCACAGCGTGAAATTGCGCTGGCTATCGGTGAGCCACCAGCCA
CCAAAGGCTACCCGCCTTCGGTCTTTGCCAAGCTTCCTGCGCTGGTTGAACGCGCAGGGAACGGCATCAGCGGCGGTGGC
TCCATCACTGCATTTTATACGGTACTGACCGAAGGCGATGACCAGCAGGACCCGATTGCCGACTCCGCGCGCGCCATTCT
CGACGGTCATATTGTGTTATCGCGTCGTCTGGCTGAAGCCGGACACTACCCGGCGATTGATATTGAAGCATCCATCAGCC
GCGCAATGACGGCGCTGATCACCGAGAAGCATTACGCCCGCGTGCGTAACTTTAAACAACTCCTCTCCAGCTTCCAGCGC
AACCGCGATCTGGTCAGCGTGGGGGCGTATGCCAAAGGCAGCGATCCTATGCTCGACAAAGCGATTAGCCTGTGGCCGCA
GCTGGAGGCGTTTTTACAACAGGGCATTTTTGAACGTGCCGACTGGGAAGATTCAATCCAGGCTCTGGAGCTGATTTTCC
CGCAGGTGTAA

Protein sequence :
MTARLTRWLNTLDNFETKMAQLPSVRRYGRLTRATGLVLEATGLQLPLGATCVIERQDGQETREVESEVVGFNGQRLFLM
PLEEVEGILPGARVYAKNISGDGLQSGKQLPLGPALLGRVLDGSGKPLDGLPAPDTTETGALITQPFNPLQRTPIEHVLD
TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTQADVIVVGLIGERGREVKDFIENILGAEGRARSVVIAAPAD
VSPLLRMQGAAYATRIAEDFRDRGKHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGG
SITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALITEKHYARVRNFKQLLSSFQR
NRDLVSVGAYAKGSDPMLDKAISLWPQLEAFLQQGIFERADWEDSIQALELIFPQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 4e-78 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-83 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 6e-83 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-83 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-83 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-82 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-83 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-83 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-70 48
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-70 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-70 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-70 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-70 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-80 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-80 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-75 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-73 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-73 46
escN AAC38383.1 EscN Virulence LEE Protein 3e-73 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-73 46
escN AAC31513.1 L0034 Virulence LEE Protein 2e-73 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-73 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-70 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-72 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-72 45
escN AAK26715.1 EscN Virulence LEE Protein 1e-72 45
escN AAL57542.1 EscN Virulence LEE Protein 1e-72 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-73 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-72 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 7e-73 45
escN CAI43874.1 EscN protein Virulence LEE Protein 5e-73 45
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-71 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-69 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-77 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-73 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 4e-73 43
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 7e-73 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-73 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 3e-73 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 9e-73 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-67 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-67 41
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-74 41
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 7e-62 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_006579293.1 flagellum-specific ATP synthase VFG2331 Protein 7e-179 85
fliI YP_006579293.1 flagellum-specific ATP synthase VFG2499 Protein 2e-146 69
fliI YP_006579293.1 flagellum-specific ATP synthase VFG1254 Protein 4e-120 63
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0515 Protein 3e-70 48
fliI YP_006579293.1 flagellum-specific ATP synthase VFG2144 Protein 3e-85 46
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0048 Protein 8e-83 46
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0812 Protein 1e-73 46
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0730 Protein 2e-73 46
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0390 Protein 2e-75 44
fliI YP_006579293.1 flagellum-specific ATP synthase VFG0192 Protein 1e-76 44
fliI YP_006579293.1 flagellum-specific ATP synthase VFG2459 Protein 1e-65 41
fliI YP_006579293.1 flagellum-specific ATP synthase VFG1008 Protein 3e-63 41