Gene Information

Name : ECENHK_08570 (ECENHK_08570)
Accession : YP_006578215.1
Strain : Enterobacter cloacae ENHKU01
Genome accession: NC_018405
Putative virulence/resistance : Virulence
Product : outer membrane usher protein SfmD
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 1847415 - 1849958 bp
Length : 2544 bp
Strand : -
Note : COG3188 P pilus assembly protein, porin PapC

DNA sequence :
ATGATCAGAGCTAAAAAAAATAAGATCTCGCCAACCAGGCTGGCCTGCTTCATCGCATTGCAGTGTACGTTCTCGTTGTC
GCACCCTGCCTGTGCCCGGGAATATTTTAACCCCGCGCTACTGGGTATTGACGGTCCAGGTAAAGACATTTCTGACCTGA
GTGCCTTTGAAGAAGGTGTTGGCCAAATGCCGGGTACTTATCGCGTTGACGTGCTGGTCAATCAGACATTGACTGGCACA
CATGACATTGAATTTAGCATGCAAAAGGACAGTCACGGCCAGACATCATTGCAACCGTGCATAAGCGTGGAGATGCTACG
TGACTTTGGCGTCCGAACCGAGCAATTTCCGCAACTGACCGGAGAGAACCATTGCGCAAACCTGGCCGCTATTCCCAACG
CTGCTACTCAGTTCGTCTTCAATAAACAGCAACTTTTGTTGAGCATCCCTCAGGCGGCGATGAATAACCAGGCGCGTGGA
TATGTTGCTCCAGAACGCCTGGATGAAGGCATCAATGCGCTGCTGCTTAATTACAGCTTTACAGGCGGCAACACCCAGGC
TCGTCACTCTGATATGACCGATTCAGACAGCTATTACCTGAACCTGCGCCCAGGGCTAAATATCGGCCCATGGCGCGTAC
GTAACTACAGCACCTGGTCACGCAACACCAGCGGCAACAGTACCCAGAATAACTGGGATTCGGCATATACCTACGCTCAG
CGAAATATTATCGCGCTCAAAAGTCAGCTGACGCTTGGCGAAAGCAACTCCCCTTCCGATCTGTTTGACAGCGTACCTTT
TCGGGGTGCGCAACTGGCCTCCGATGACGACATGCTGCCTGACAGCCTGCGCGGCTACGCACCGGTGGTTCGCGGAATTG
CCCGGACCAACGCCCAGGTCATCATTAAACAAAACGGCTACACCATTTACCAAAGCTATGTGTCGCCGGGGGCATTTGAG
ATCACTGACATGTATCCCACGGGCAGTAGCGGCGATCTGGACGTGACGATTAAAGAAGCTGACGGCAGCGAACAGCACCA
GTTGGTCCCCTTCGCCTCACTGCCGGTTCTACAGCGTGAAGGTCGTTTAAAGTACAGCCTGACCAGCGGTCAGTACCGTC
CTTACGACAGCGCCGTCGATAAAACATTCTTTACTCAGGGCTCGGCCATCTACGGCCTGCCAGCAGGATTTACCGCCTAC
GGCGGCGGACAGCTATCGCAACATTATCAGTCGCTACTTGTGGGGATCGGTAAAAACTTTGGTGGGCTGGGAGCCGTATC
CGTCGATGGCACGCAGGCCTGGTCAACCCAACAGGATCAGGAAAAAGCGATCGGCCAGTCAATGCGGGTTCGTTACAGTA
AAAACATTCTCGAAACGGGGACCAATATCGCCATTTCGGGTTATCGCTACTCAACTTCAGGCTATTACACCCTGCAGGAA
GTACTCGACACCTGGCGGGACAGTAACTGGCAGGACAATACCGAACGCCGGCGTAATCGTGCCGAGATATTGCTTAACCA
GAATCTCTGGGAAGGCGCGGGGTCCATCTCGTTAAACGCCATCAGCGAGGATTACTGGAACAGCGACCGAACAATGCGCT
CAATCGGCGCGGGTTACAGCAACAGCTGGAACGGCATCACCTGGAGCCTGAACTACAGCTTTAACCGTAACACCACTGAT
GCCCGCGACAGCGACACCGTTTACAGCGAGGACCAGGTGTTCTCAATGAGTATCAGCGTGCCGCTGGATCGCTGGCTCAG
CAGCAGTCGCGCCACCTACAACCTCAATACCAGTAAACATGGCAGCACCAGCCATACGGTCGGGCTGAATGGCACCGCGC
TGCAAGATCAGAACCTCAACTGGAGCGTGCAGGAAAGCGAGACGAACCACGATGGGGGTAACGGTGGTAGTGCCAGCCTG
GACTATCAGGGTACCTATGCCAGAATGAACAGCGCCTACAGTTACGACCACGATCAGCGCCGGGTAAATTATGGTATCAG
CGGCGGTGTGGTTGCGCATGCAAATGGCATTACGCTGAGTCAGGAATTAGGTGAGACCGTCGCTCTGGTTGAAGCGCCGG
GAGCAAGCGGTGTTGATATCACTAACCAGACGGGCGTCAGCACCGATTTCCGCGGCTATACCGTTGTGCCGTTTATCAGC
CCATACCGTGAAAACACGCTGTCGCTGAATACCGAACAACTGCCGGATGATGCCGACGTTGAGCTTTCTGCGAAAACGGT
TACGCCAACCCGAGGGGCTATTGTCCGGGCACATTTTGACACACGTGTGGGTAAACGCGTTTTGATGACGTTAACCCGTC
GTGACGGGCGCCCTGTTCCGTTTGGTGCAATCGTGACTGACGACAAGAACGCAAAAACCACCTCGATTGTCGGTGATGGT
GGACAGGTTTATCTGGCTGGTATGTCACTTCAGGGAAGTGCAACCGTTAAATGGGGTGCAAATAAGCAGTGTCATACCCG
CTGGCAATTACCAGAGAGCACATCATCAAGCGCAATTGTAGAGGTCAACGCATCATGTCGTTAA

Protein sequence :
MIRAKKNKISPTRLACFIALQCTFSLSHPACAREYFNPALLGIDGPGKDISDLSAFEEGVGQMPGTYRVDVLVNQTLTGT
HDIEFSMQKDSHGQTSLQPCISVEMLRDFGVRTEQFPQLTGENHCANLAAIPNAATQFVFNKQQLLLSIPQAAMNNQARG
YVAPERLDEGINALLLNYSFTGGNTQARHSDMTDSDSYYLNLRPGLNIGPWRVRNYSTWSRNTSGNSTQNNWDSAYTYAQ
RNIIALKSQLTLGESNSPSDLFDSVPFRGAQLASDDDMLPDSLRGYAPVVRGIARTNAQVIIKQNGYTIYQSYVSPGAFE
ITDMYPTGSSGDLDVTIKEADGSEQHQLVPFASLPVLQREGRLKYSLTSGQYRPYDSAVDKTFFTQGSAIYGLPAGFTAY
GGGQLSQHYQSLLVGIGKNFGGLGAVSVDGTQAWSTQQDQEKAIGQSMRVRYSKNILETGTNIAISGYRYSTSGYYTLQE
VLDTWRDSNWQDNTERRRNRAEILLNQNLWEGAGSISLNAISEDYWNSDRTMRSIGAGYSNSWNGITWSLNYSFNRNTTD
ARDSDTVYSEDQVFSMSISVPLDRWLSSSRATYNLNTSKHGSTSHTVGLNGTALQDQNLNWSVQESETNHDGGNGGSASL
DYQGTYARMNSAYSYDHDQRRVNYGISGGVVAHANGITLSQELGETVALVEAPGASGVDITNQTGVSTDFRGYTVVPFIS
PYRENTLSLNTEQLPDDADVELSAKTVTPTRGAIVRAHFDTRVGKRVLMTLTRRDGRPVPFGAIVTDDKNAKTTSIVGDG
GQVYLAGMSLQGSATVKWGANKQCHTRWQLPESTSSSAIVEVNASCR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
sfaF CAC16953.2 SfaF protein Virulence PAI III 536 Protein 2e-154 43
fim2D AFH78429.1 putative fimbrial usher protein Virulence KpGI-5 Protein 5e-149 42
safC NP_454907.1 outer-membrane fimbrial usher protein Not tested SPI-6 Protein 2e-142 42
afaC ADD91743.1 putative AfaC-VIII usher protein Not tested PAI-I AL862 Protein 2e-143 41
lpfC YP_006098365.1 fimbrial outer membrane usher protein Not tested Tn2411 Protein 6e-135 41
lpfC NP_839216.1 long polar fimbriae Not tested SHI-2 Protein 4e-134 41
lpfC NP_709462.1 long polar fimbriae Not tested SHI-2 Protein 4e-134 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0454 Protein 3e-164 46
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0446 Protein 1e-146 43
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG1645 Protein 1e-154 43
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0912 Protein 2e-154 43
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0944 Protein 3e-148 42
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0853 Protein 2e-140 42
ECENHK_08570 YP_006578215.1 outer membrane usher protein SfmD VFG0876 Protein 3e-154 42