Gene Information

Name : fliI (PADK2_20160)
Accession : YP_006484005.1
Strain : Pseudomonas aeruginosa DK2
Genome accession: NC_018080
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 4344632 - 4345987 bp
Length : 1356 bp
Strand : -
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGCGCCTTGAGCGAACCAGTTTCGCCAGGCGCCTGGAGGGCTACACCGAGGCTGTCTCGCTGCCGGCGCAGCCGGTGGT
CGAGGGCCGCCTGCTGCGCATGGTCGGCCTGACCCTCGAAGCCGAAGGCCTGCAGGCCGCGGTCGGCAGCCGCTGCAACG
TGATCAACGAAAGCGGCTACCACCCGGTGCAGGTGGAGGCCGAAGTGATGGGCTTTTCCGGTAGCAAGGTGTACCTGATG
CCGGTCGGCAGCCTCGCCGGCATCGCGCCTGGCGCGCGGGTCGTGCCGCTGCCGGACACCGGGCGCCTGCCGATGGGCAT
GTCGATGCTCGGCCGGGTGCTCGACGGCGCCGGCCGCGCCCTCGACGGCAAGGGCGGGATGCGCGCCGAAGACTGGGTGC
CGATGGACGGCCCGACGATCAACCCGCTCAAGCGCCACCCGATCAGCGAACCTCTCGACGTCGGCATCCGCTCGATCAAT
GGCCTGCTCACGGTCGGCCGCGGCCAGCGCCTCGGCCTGTTCGCCGGTACCGGGGTGGGCAAGTCGGTGCTGCTGGGGAT
GATGACCCGTTTCACCAGGGCCGACATCATCGTCGTCGGTCTGATCGGCGAGCGGGGTCGCGAGGTGAAGGAGTTCATCG
ACGAGATCCTCGGCGAGGAAGGCCTCAAGCGCTCCGTGGTGGTCGCCTCGCCGGCCGACGACGCGCCGCTGATGCGCCTG
CGCGCCGCGCAATACTGCACGCGGATCGCCGAGTACTTCCGCGACAAAGGCAAGAACGTATTGCTGCTGATGGACTCGCT
GACCCGCTACGCCCAGGCCCAGCGCGAGATCGCCCTGGCCATCGGCGAGCCGCCGGCGACCAAGGGGTATCCGCCATCGG
TGTTCGCCAAGCTGCCGAAGCTGGTGGAACGCGCCGGCAATGCCGAAGCCGGTGGCGGCTCAATCACCGCGTTCTACACC
GTGCTCTCCGAGGGCGACGACCAGCAGGACCCGATCGCCGACGCCGCTCGCGGCGTGCTCGACGGGCACTTCGTATTGTC
CCGGCGGCTGGCCGAGGAAGGCCACTATCCGGCCATCGATATCGAAGCTTCGATCAGCCGGGTGATGCCGCAGGTGGTCG
AGGCCGAGCACCTGCGCGACGCCCAGCGCTTCAAGCAGCTCTGGTCGCGCTACCAGCAGAGTCGCGACCTGATCAGCGTC
GGTGCCTACGTGGCCGGCGGCGATCCCGAGACCGACCTGGCCATCGCACGCTTCCCGGTCATGCGCCAGTTCCTGCGCCA
GGGCCTCGACGAAAGCGAAAGCCTGGCGGAAAGCCGTGCGCGGCTGGCGAGCCTGCTCGCCGGCGGGCAGGCCTGA

Protein sequence :
MRLERTSFARRLEGYTEAVSLPAQPVVEGRLLRMVGLTLEAEGLQAAVGSRCNVINESGYHPVQVEAEVMGFSGSKVYLM
PVGSLAGIAPGARVVPLPDTGRLPMGMSMLGRVLDGAGRALDGKGGMRAEDWVPMDGPTINPLKRHPISEPLDVGIRSIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTRADIIVVGLIGERGREVKEFIDEILGEEGLKRSVVVASPADDAPLMRL
RAAQYCTRIAEYFRDKGKNVLLLMDSLTRYAQAQREIALAIGEPPATKGYPPSVFAKLPKLVERAGNAEAGGGSITAFYT
VLSEGDDQQDPIADAARGVLDGHFVLSRRLAEEGHYPAIDIEASISRVMPQVVEAEHLRDAQRFKQLWSRYQQSRDLISV
GAYVAGGDPETDLAIARFPVMRQFLRQGLDESESLAESRARLASLLAGGQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-77 52
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-76 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-76 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-76 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-76 51
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-92 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-93 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-93 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-92 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 5e-93 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 5e-93 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-93 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-86 50
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-73 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-90 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-90 49
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-75 49
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-77 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 9e-73 48
escN AAK26715.1 EscN Virulence LEE Protein 1e-74 48
escN AAL57542.1 EscN Virulence LEE Protein 1e-74 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 7e-75 48
escN CAI43874.1 EscN protein Virulence LEE Protein 5e-75 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-75 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-75 48
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 7e-69 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-82 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 5e-69 47
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-69 47
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 9e-71 47
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 8e-71 47
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-71 47
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-68 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 9e-69 46
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-66 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-66 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-76 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-76 45
escN AAC38383.1 EscN Virulence LEE Protein 6e-76 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-76 45
escN AAC31513.1 L0034 Virulence LEE Protein 3e-76 45
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-71 45
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-61 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-61 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-61 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-61 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 6e-66 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-62 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_006484005.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 100
fliI YP_006484005.1 flagellum-specific ATP synthase VFG2331 Protein 3e-120 64
fliI YP_006484005.1 flagellum-specific ATP synthase VFG2499 Protein 1e-123 64
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0515 Protein 7e-77 51
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0192 Protein 4e-88 48
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0048 Protein 1e-86 48
fliI YP_006484005.1 flagellum-specific ATP synthase VFG2144 Protein 4e-84 47
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0390 Protein 1e-82 47
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0730 Protein 3e-76 45
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0812 Protein 2e-76 45
fliI YP_006484005.1 flagellum-specific ATP synthase VFG0555 Protein 1e-61 43
fliI YP_006484005.1 flagellum-specific ATP synthase VFG1008 Protein 4e-62 41
fliI YP_006484005.1 flagellum-specific ATP synthase VFG2459 Protein 1e-65 41