Gene Information

Name : fliI (A3UG_14395)
Accession : YP_006478307.1
Strain : Enterobacter cloacae SDM
Genome accession: NC_018079
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 3089239 - 3090609 bp
Length : 1371 bp
Strand : +
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGACCGCACGCCTCACTCGCTGGCTCAACACGCTCGATAACTTTGAAACGAAGATGGCGCAACTGCCATCTGTTCGTCG
TTATGGGCGCCTGACCCGTGCCACCGGTCTGGTGCTGGAAGCCACCGGCCTGCAGCTGCCTCTGGGGGCCACCTGCGTAA
TCGAGCGCCAGGATGGGCAGGAAACGCGGGAAGTGGAAAGCGAAGTGGTGGGCTTTAACGGCCAGCGTCTTTTCCTGATG
CCGCTTGAAGAGGTGGAAGGCATTCTGCCCGGCGCGCGCGTCTATGCGAAAAACATCATCGGCGATGGTCTGCAAAGCGG
TAAGCAGCTCCCGCTCGGCCCCGCCCTGCTGGGCCGCGTGCTGGACGGCAGCGGTAAACCGCTCGACGGCCTGCCCGCTC
CAGACACCACCGAAACCGGCGCGCTGATCACCCAACCATTTAACCCCCTGCAACGTACCCCGATCGAACATGTTCTTGAT
ACCGGCGTGCGCCCGATCAACGCCCTGCTGACCGTCGGTCGCGGCCAGCGTATGGGGCTGTTCGCCGGCTCAGGGGTCGG
TAAATCGGTCCTGCTCGGCATGATGGCCCGTTACACCCAGGCCGATGTGATCGTCGTGGGGCTGATCGGTGAACGTGGTC
GTGAAGTTAAAGACTTCATTGAAAACATTCTGGGTGCGGAAGGCCGCGCCCGCTCGGTGGTCATCGCCGCCCCGGCGGAT
GTCTCGCCGTTGCTGCGTATGCAGGGGGCAGCCTATGCCACCCGTATCGCCGAAGATTTCCGTGACCGTGGTCAGCACGT
GCTGCTGATCATGGACTCCCTGACCCGTTACGCGATGGCGCAGCGTGAAATCGCTCTCGCCATCGGTGAGCCACCGGCCA
CCAAAGGCTATCCGCCGTCGGTCTTTGCCAAACTCCCGGCGCTGGTTGAACGCGCAGGAAACGGCATCAGCGGCGGTGGC
TCCATCACCGCGTTTTATACGGTGCTGACCGAAGGCGACGACCAGCAGGACCCGATTGCCGACTCCGCGCGTGCGATCCT
CGACGGTCATATTGTGTTATCCCGCCGGCTGGCGGAAGCCGGACACTATCCGGCGATTGATATTGAAGCATCCATCAGCC
GCGCAATGACGGCGCTGATAACCGAGAAGCACTACGCCCGCGTGCGTAACTTCAAACAACTGCTCTCCAGCTTCCAGCGC
AACCGCGATCTGGTGAGCGTGGGGGCGTATGCCAAAGGCAGCGATCCCATGCTCGACAAAGCGATTACCCTGTGGCCGCA
GCTGGAGGCATTTTTGCAACAAGGCATTTTTGAACGCGCCGACTGGGAAGATTCAATTCAGGCTCTGGAGCTGATTTTCC
CGCAGGTGTAA

Protein sequence :
MTARLTRWLNTLDNFETKMAQLPSVRRYGRLTRATGLVLEATGLQLPLGATCVIERQDGQETREVESEVVGFNGQRLFLM
PLEEVEGILPGARVYAKNIIGDGLQSGKQLPLGPALLGRVLDGSGKPLDGLPAPDTTETGALITQPFNPLQRTPIEHVLD
TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTQADVIVVGLIGERGREVKDFIENILGAEGRARSVVIAAPAD
VSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGG
SITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALITEKHYARVRNFKQLLSSFQR
NRDLVSVGAYAKGSDPMLDKAITLWPQLEAFLQQGIFERADWEDSIQALELIFPQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-78 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-82 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-83 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 6e-83 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-83 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-83 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-83 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-83 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-69 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-69 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-69 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-69 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-80 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-80 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-69 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 7e-75 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-70 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-72 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-72 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-72 45
escN AAC38383.1 EscN Virulence LEE Protein 2e-72 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-72 45
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-72 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-72 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-72 45
escN AAC31513.1 L0034 Virulence LEE Protein 1e-72 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-72 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 6e-72 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-69 44
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-70 44
escN AAK26715.1 EscN Virulence LEE Protein 5e-72 44
escN AAL57542.1 EscN Virulence LEE Protein 5e-72 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 5e-72 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-73 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-73 43
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-73 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-76 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-72 41
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-73 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-72 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-67 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-67 41
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-73 41
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-61 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_006478307.1 flagellum-specific ATP synthase VFG2331 Protein 6e-179 85
fliI YP_006478307.1 flagellum-specific ATP synthase VFG2499 Protein 2e-146 69
fliI YP_006478307.1 flagellum-specific ATP synthase VFG1254 Protein 1e-119 63
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0515 Protein 1e-69 48
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0048 Protein 6e-83 46
fliI YP_006478307.1 flagellum-specific ATP synthase VFG2144 Protein 5e-85 45
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0812 Protein 6e-73 45
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0730 Protein 1e-72 45
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0390 Protein 2e-75 44
fliI YP_006478307.1 flagellum-specific ATP synthase VFG0192 Protein 3e-76 44
fliI YP_006478307.1 flagellum-specific ATP synthase VFG2459 Protein 2e-65 41
fliI YP_006478307.1 flagellum-specific ATP synthase VFG1008 Protein 5e-64 41