Gene Information

Name : A458_03965 (A458_03965)
Accession : YP_006456468.1
Strain : Pseudomonas stutzeri CCUG 29243
Genome accession: NC_018028
Putative virulence/resistance : Unknown
Product : type I restriction-modification enzyme, R subunit
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 881059 - 884160 bp
Length : 3102 bp
Strand : +
Note : COG0610 Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

DNA sequence :
ATGACCGAAGACCAACTGGAACAGGAATGCCTTGGCTGGCTGGGCGAACTGGGTTACACGCACCTTTACGGGCCGGATAT
CGCCCATGACGGCGGCGACCCGCAGCGCGACAGTTATCGCCAGGTCGTGCTGACGGAACGCCTGCGCGCCGCCATCGCCA
AGCTCAATCCCCAGGTGCCGCTGGCCGCTCGCGAGGATGCGCTCAAGCAGGTAGTGGATCTGGGTGTGCCGGCGCAGTTG
TCGGCCAACCGCCTGTTCCATCGGCTGCTGGTCGGCGGCGTGCCGGTGCAGTACCAGCTCGATGGCGAAACCCGGGGTGA
TTTCGTGCGGCTGATCGACTGGGTAAACGTGCGCGGCAACGAGTGGCTGGCGATCAACCAGTTCAGCATTCAGGGGCCGA
AGCACACCCGGCGCCCCGACATCATCCTGTTCGTCAACGGCCTGCCGCTGGTGCTGCTGGAGCTGAAGAACCCCGCCGAT
GTGAACGCCGACCTGTGGAAGGCCTTCGACCAGTTGCAGACCTATAAGGAGCAGATTCCCGATCTGTTCCACTACAACGA
AATCCTGGTGATTTCCGATGGCAGCGAGGCGCGCATGGGCTCGCTGTCGGCCGATGCCGAGCGCTTCATGAACTGGCGCA
CCATCGATGGTGAGCAGCTCGACCCGCTGGGTCAGTTCAACGAACTGGAAACCCTTCTGCGCGGCGTGATGGCGCCGGAG
ATGCTGCTGGATTACCTGCGTTACTTCGTGCTGTTCGAGGATGACGGTCGCTTGGTGAAAAAGATCGCCGGTTATCACCA
GTTCCACGCGGTGCGGGCGGCGATCCGCCAGGTGGTGACCGCTTCGCGCCCGGGCGGCACGCACAAGGGCGGGGTGGTCT
GGCATACCCAGGGCTCGGGTAAGAGCATCACCATGACCTGTTTTGCCGCACGGGTGATGCAGGCGGCGGCGATGGAGAAC
CCGACCATCGTGGTGATCACCGACCGCAACGACCTGGACGGCCAGTTGTTCGGGGTGTTTTCGCTGTCGCATGACTTGCT
TCGCGAGCAACCGGTGCAGGCCGAGACGCGTGGCGATCTGCGGGAGAAACTAGGCAACCGTCCGAGTGGCGGCATCGTGT
TTGCCACCATTCAGAAGTTTATGCCGGGTGAGGATGAAGACAGCTTCCCGGTGCTTTCGACCCGCTCGAACATCGTGGTG
ATCGCAGACGAGGCGCATCGCACCCAATATGGCTTCCATGCCTCGCTGAAGGCGCCCGACCTGAAGGTGGCCGAGGCCAG
CGCCCGTTACCAGGTGGGCTATGCCCAGCACCTGCGCGACGCCCTGCCGAACGCCACCTTTGTGGCCTTTACCGGTACGC
CGGTATCCAGTGAAGACCGTGACACCCGGGCGGTGTTCGGTGACTACATCCACGTCTACGACATGCAGCAGGCCAAGGAC
GACGGCGCCACGGTGGCGATCTACTACGAATCGCGGCTGGCCAAGCTGTCGCTGAAAGACAGTGAGCTGGCACATATCGA
TGATGAAGTGGACGAGCTGGCCGAAGATGAGGAAGAGGATCAGCAATCGCGCCTGAAATCGCGCTGGGCGGCGCTGGAAA
AGGTCGTGGGTGCCGAGCCGCGTATCAAAAGCGTGGCGGCGGATCTGGTCGCGCACTTCGAGGAGCGCAATCAAGCGCAG
AGCGGCAAGGCCATGATCGTGGCCATGAGCCGCGAGATCTGTGTGCATCTGTACAACGAGATCGTCGCGCTGCGCCCCGA
ATGGCACGACGAAGACCCGGAGCAGGGCGCGATCAAGGTGGTGATGACCGGCAGCGCTTCGGACAAGGCGTTGCTGCGCC
CGCATATCTATCCGAGCCAGGTGAAAAAACGCCTGGAGAAACGCTTCAAGGACCCGAAAGACCCGCTGCAGCTGGTCATC
GTGCGCGACATGTGGCTGACCGGCTTCGACGCGCCCTGCGTGCATACCCTGTATGTCGACAAGCCCATGAAGGGCCACAA
CCTGATGCAGGCCATCGCCCGGGTGAACCGCGTATTCAAGGACAAGCAGGGCGGCCTGGTGGTGGATTACATCGGCATTG
CCAACGAACTGAAGGCTGCGCTCAAGGAGTACACCGCCAGCAAGGGGCGCGGCCGGCCAACCGTGGATGCCCACGAAGCC
TACTCAGTACTGGAGGAAAAGCTCGATGTGTTGCGCAGCCTGCTGCATGGCTTCGACTACAGCGATTACCTGACCGGCGG
CCACAAGCTGCTGGCCGGTGCGGCCAACCATGTGCTGGGCCTGGAGGATGGCAAGAAGCGCTTCGCCGACAACGCGCTGG
CCATGAGCAAGGCCTTCACCCTGTGCTGCACGCTGGATGAAGCCAAGGCGGTGCGCGAGGAGGTGGCCTTTCTGCAGGCC
ATCAAGGTGCTGCTGACCAAGCGCGAGATCAGTGCCAAGAAGAAGAGCGACGAGGAACGCGAACTGGCTATCCGCCAGAT
CATCGGCAATGCCGTGGTCTCCGGCGAGGTGGTGGATGTGTTCGAAGCGGTGGGCCTGGACAAGCCCAACATCGGCCTCT
TGGACGATGAGTTCCTCGCTGAGGTACGCAACCTGCCTGAGAGGAACCTGGCGGTGGAGCTGCTGGAGCGGCTGCTGGAA
GGCGAGATCAAGAGCAAGTTTGCCAGCAACCTGGCACAGGAAAAGAAATTCTCCGAGCTGCTCGACAACGTGATCAAGCG
CTACCAGAACCGCTCCATCGAAACCGCTCAGGTCATCGAAGAACTGATCGAGATGGCCAAGAAGTTCGCCGCCGCCAGCA
AGCGTGGCGATGAACTAGGGCTTAATGACGACGAGCTGGCCTTCTACGATGCTCTGGCCAATAACGAGGCATCCGTTCGC
GAGCTGGGCGACGAGATCCTCGCTAAGATCGCCCACGAGCTGACCGCCAGCCTGCGGCAGAACGTGACGGTGGACTGGAG
CAACCGCGACAGCGTACGTGCGAAGCTGCGGCTAATGGTCAAACGCATCCTACGGAAATATAAATATCCGCCGGATCAGC
AGGAAGGCGCTGCACAGCTGATTTTGGAGCAGGCTGAGATGCTATGTGCGGGTTGGGCGTAG

Protein sequence :
MTEDQLEQECLGWLGELGYTHLYGPDIAHDGGDPQRDSYRQVVLTERLRAAIAKLNPQVPLAAREDALKQVVDLGVPAQL
SANRLFHRLLVGGVPVQYQLDGETRGDFVRLIDWVNVRGNEWLAINQFSIQGPKHTRRPDIILFVNGLPLVLLELKNPAD
VNADLWKAFDQLQTYKEQIPDLFHYNEILVISDGSEARMGSLSADAERFMNWRTIDGEQLDPLGQFNELETLLRGVMAPE
MLLDYLRYFVLFEDDGRLVKKIAGYHQFHAVRAAIRQVVTASRPGGTHKGGVVWHTQGSGKSITMTCFAARVMQAAAMEN
PTIVVITDRNDLDGQLFGVFSLSHDLLREQPVQAETRGDLREKLGNRPSGGIVFATIQKFMPGEDEDSFPVLSTRSNIVV
IADEAHRTQYGFHASLKAPDLKVAEASARYQVGYAQHLRDALPNATFVAFTGTPVSSEDRDTRAVFGDYIHVYDMQQAKD
DGATVAIYYESRLAKLSLKDSELAHIDDEVDELAEDEEEDQQSRLKSRWAALEKVVGAEPRIKSVAADLVAHFEERNQAQ
SGKAMIVAMSREICVHLYNEIVALRPEWHDEDPEQGAIKVVMTGSASDKALLRPHIYPSQVKKRLEKRFKDPKDPLQLVI
VRDMWLTGFDAPCVHTLYVDKPMKGHNLMQAIARVNRVFKDKQGGLVVDYIGIANELKAALKEYTASKGRGRPTVDAHEA
YSVLEEKLDVLRSLLHGFDYSDYLTGGHKLLAGAANHVLGLEDGKKRFADNALAMSKAFTLCCTLDEAKAVREEVAFLQA
IKVLLTKREISAKKKSDEERELAIRQIIGNAVVSGEVVDVFEAVGLDKPNIGLLDDEFLAEVRNLPERNLAVELLERLLE
GEIKSKFASNLAQEKKFSELLDNVIKRYQNRSIETAQVIEELIEMAKKFAAASKRGDELGLNDDELAFYDALANNEASVR
ELGDEILAKIAHELTASLRQNVTVDWSNRDSVRAKLRLMVKRILRKYKYPPDQQEGAAQLILEQAEMLCAGWA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
api52 CAF28526.1 hsdr-like Type I restriction enzyme Not tested YAPI Protein 0.0 58
hsdR BAH57699.1 type I restriction-modification system endonuclease homologue Not tested Type-VII SCCmec Protein 0.0 48
hsdR BAD24840.1 type I restriction-modification system endonuclease homologue Not tested Type-V SCCmec Protein 0.0 48
hsdR YP_251977.1 type I restriction-modification system restriction subunit Not tested SCCmec Protein 0.0 47