Gene Information

Name : fliI (A458_13700)
Accession : YP_006458394.1
Strain : Pseudomonas stutzeri CCUG 29243
Genome accession: NC_018028
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2995112 - 2996470 bp
Length : 1359 bp
Strand : -
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGCGCCTTGAGCGAACCAGTTTCGCCAAGCGATTCGAAGCCTATGGCGAAGCGATCAAACTGCCCAGCCAGCCCATGCT
TGAAGGGCGCCTGCTGCGCATGGTCGGTCTGACGCTGGAGGCCGAAGGGCTGCGCGCAGCCGTTGGCAGTCGCTGCCTGG
TGATCAACGACGACAGTTATCACCCCACCGAGGTCGAGGCCGAGGTGATGGGCTTTTCCAGCGGCAAGCTGTATCTGATG
CCTGTCGGCAGCCTTGCCGGTATCGCGCCGGGCGCGCGGGTCGTGCCGCTGGTCAATACCGGACGGCTGCCCATGGGCAT
GTCCATGCTCGGCCGCGTGCTGGATGGCGCAGGGCGTGCACTGGACGGCAAGGGCGGCATGAGAGCGGAGGATTGGGTGC
CGATGGACGGCCCCGTTATCAACCCGCTCAAGCGCCACCCCATCAGCGAGCCGCTGGATGTCGGCATCCGCAGTATCAAT
GGCTTGCTGACCGTCGGTCGCGGTCAGCGCCTAGGCCTATTTGCCGGCACCGGTGTCGGCAAGTCGGTGCTGCTGGGCAT
GATGACCCGCTTCACCGAGGCCGACATCATCGTCGTCGGCCTGATCGGCGAGCGGGGGCGAGAGGTCAAGGAATTCATCG
AGAACATCCTTGGTGAGGAGAGCATCAAACGCTCGGTGGTAGTGGCCTCGCCCGCTGACGAGGCGCCGCTCATGCGCTTG
CGCGCCGCGCAGTACTGCACGCGTATTGCGGAGTACTTCCGCGATCAGGGCAAGAACGTGTTGCTGCTGATGGATTCGCT
GACGCGCTATGCCCAGGCCCAGCGCGAGATCGCCCTGGCCATCGGCGAGCCGCCAGCGACCAAGGGCTATCCGCCGTCGG
TGTTCGCCAAGCTGCCGAGTCTGGTCGAGCGCGCCGGTAATGCCGAGCAGGGCGGCGGCTCGATCACGGCGTTCTACACC
GTGCTCTCCGAGGGTGACGACCAGCAGGACCCGATCGCCGATGCCGCTCGCGGTGTGCTCGACGGCCACATCGTGCTGTC
GCGCCGTCTGGCCGAAGAAGGCCACTATCCGGCTATCGACATCGAAGCCTCCATCAGCCGGGTCATGCCGCAGGTGGTGA
GCGCCGAACATGTGCGTGCGTCGCAGCGTTTCAAGCAGCTCTGGTCGCGCTACCAGCAGAGCCGCGACCTGATCAGTGTC
GGCGCCTATGTGCCAGGTGGCGATCCGGAAACGGATATGGCCATCGCGCGCCAGGCCGAGATGGTCAGTTACCTGCGCCA
GGGCCTCGATGAAAGCGAGGACCTGACGCGCAGCGCCGCGCAACTCGCAGCTGTGTTCAATCCACGGCAGGGCGGCTAA

Protein sequence :
MRLERTSFAKRFEAYGEAIKLPSQPMLEGRLLRMVGLTLEAEGLRAAVGSRCLVINDDSYHPTEVEAEVMGFSSGKLYLM
PVGSLAGIAPGARVVPLVNTGRLPMGMSMLGRVLDGAGRALDGKGGMRAEDWVPMDGPVINPLKRHPISEPLDVGIRSIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEADIIVVGLIGERGREVKEFIENILGEESIKRSVVVASPADEAPLMRL
RAAQYCTRIAEYFRDQGKNVLLLMDSLTRYAQAQREIALAIGEPPATKGYPPSVFAKLPSLVERAGNAEQGGGSITAFYT
VLSEGDDQQDPIADAARGVLDGHIVLSRRLAEEGHYPAIDIEASISRVMPQVVSAEHVRASQRFKQLWSRYQQSRDLISV
GAYVPGGDPETDMAIARQAEMVSYLRQGLDESEDLTRSAAQLAAVFNPRQGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-83 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-78 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-78 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-78 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-78 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-78 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-88 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-88 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 4e-91 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-91 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-91 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-90 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-91 48
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-75 48
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 9e-76 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-76 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-85 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-69 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-89 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-89 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-80 47
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-75 47
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-67 47
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-67 47
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-75 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-70 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-70 46
escN AAC38383.1 EscN Virulence LEE Protein 2e-76 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-76 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-76 46
escN AAC31513.1 L0034 Virulence LEE Protein 1e-76 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-76 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-76 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-76 46
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-76 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-76 46
escN AAK26715.1 EscN Virulence LEE Protein 6e-76 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-76 46
escN AAL57542.1 EscN Virulence LEE Protein 6e-76 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 3e-70 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 7e-72 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 7e-72 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-69 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-73 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 4e-63 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_006458394.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 88
fliI YP_006458394.1 flagellum-specific ATP synthase VFG2331 Protein 4e-122 62
fliI YP_006458394.1 flagellum-specific ATP synthase VFG2499 Protein 1e-124 62
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0515 Protein 2e-78 50
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0048 Protein 2e-90 47
fliI YP_006458394.1 flagellum-specific ATP synthase VFG2144 Protein 4e-87 47
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0192 Protein 2e-88 47
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0390 Protein 2e-82 47
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0812 Protein 8e-77 46
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0730 Protein 1e-76 46
fliI YP_006458394.1 flagellum-specific ATP synthase VFG0555 Protein 2e-63 42
fliI YP_006458394.1 flagellum-specific ATP synthase VFG2459 Protein 4e-67 41
fliI YP_006458394.1 flagellum-specific ATP synthase VFG1008 Protein 2e-61 41