Gene Information

Name : rscN (PflA506_0698)
Accession : YP_006322227.1
Strain : Pseudomonas fluorescens A506
Genome accession: NC_017911
Putative virulence/resistance : Virulence
Product : type III secretion ATP synthase RscN
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 816370 - 817728 bp
Length : 1359 bp
Strand : +
Note : identified by similarity to SP:Q8RK01; match to protein family HMM PF00006; match to protein family HMM PF02874; match to protein family HMM TIGR01026

DNA sequence :
GTGAGTACGCAATTGCAAGCGCGTCTCGACGCCTGGCAACAGCGCCAGGCCCAGGCGCTGAGCAGCTTTGCGCCAGTGGC
GATGCGTGGTCGCATCCAGCGCGTCAATGGCATGTTGCTGCAATGCCGATTGCCTCAGGCGCGCATCGGCGATTTGTGCC
AAGTGGAAAAAGGGCCGGGCGATTTCATGCTCGCCGAAATCATCGGCTTTGATCAGCAGGATGCAGTACTCAGTGCCCTG
GGTAATCTGGAAGGCGTACGAGTGGGCGCTGGCGTTGAGCGCCTGGGCGTTTCGCATCGTGTGCAGGTCAGCGAGGCGCT
GTTGGGCCAAGTACTGGATGGTTTCGGGCGACCGATTACGGGGCAGGGCGCCAGTGCGTTTGTCGAAGCTGAACTGCCCG
ATACCAGTGCGGTGCTGTGTGAAGCGCCGTTACCCACCGAGCGCCCGCGGATCAACCGTGCCCTGGCCACTGGGGTGCGT
TCCATTGATGGCTTGATGACGCTGGGTGAAGGCCAGCGCGTCGGTCTGTTCGCCGGGGCCGGTTGCGGCAAGACGACTCT
GTTGGCCGAGATCGCCCGTAACGTCGAATGCGACGTCATCGTGTTCGGCCTGATCGGCGAGCGAGGCCGCGAGTTGCGTG
AGTTTCTCGACCATGAGCTGGATGAGCAACTGCGCTCCAAAGCGGTACTGGTGTGTGCTACGTCTGATCGCTCCAGCATG
GAGCGCGCTCGCGCTGCATTCACCGCCACGGCGTTGGCTGAAGGTTTCCGGCGCAAGGGTCAACGGGTGTTGTTGCTGAT
TGATTCCCTGACTCGTTTCGCCAGGGCCCAGCGCGAAATTGGCCTGGCTGCCGGTGAGCCCCTGGGGCGCGGTGGTCTGC
CGCCTTCGGTGTACAGCTTGCTGCCGCGTCTGGTGGAACGCGCCGGGTTGACCCGTGACGGTGTGATCACAGCGATCTAC
ACGGTGCTGATCGAGCAGGACTCCATGAACGACCCAGTGGCCGACGAAGTGCGTTCGTTGCTTGACGGCCATATCGTGCT
GTCGCGCAAATTGGCCGAGCGTGGGCACTATCCGGCGGTGGATGTGCTGGCGAGCCTTTCACGAATATTGAGCAACGTCG
CAGAACCTGCGGATATCCAGGCGGGCACGGCGTTGCGGCGTTTGCTATCGGCGTACCAGCAGATTGAGTTGATGCTCAAG
CTGGGCGAGTACCAGCCTGGCAGCGATGCCCTGACGGACCTGGCGGTGAACAGTCGCCAAGCGGTGGATGGTTTCCTGCG
CCAGGATTTGCGTGAGCCGACACCGATGGCTGTCACGCTGGAGCAACTCAAGGAGCTGACCGCCCATGTCCCATTCTGA

Protein sequence :
MSTQLQARLDAWQQRQAQALSSFAPVAMRGRIQRVNGMLLQCRLPQARIGDLCQVEKGPGDFMLAEIIGFDQQDAVLSAL
GNLEGVRVGAGVERLGVSHRVQVSEALLGQVLDGFGRPITGQGASAFVEAELPDTSAVLCEAPLPTERPRINRALATGVR
SIDGLMTLGEGQRVGLFAGAGCGKTTLLAEIARNVECDVIVFGLIGERGRELREFLDHELDEQLRSKAVLVCATSDRSSM
ERARAAFTATALAEGFRRKGQRVLLLIDSLTRFARAQREIGLAAGEPLGRGGLPPSVYSLLPRLVERAGLTRDGVITAIY
TVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAVDVLASLSRILSNVAEPADIQAGTALRRLLSAYQQIELMLK
LGEYQPGSDALTDLAVNSRQAVDGFLRQDLREPTPMAVTLEQLKELTAHVPF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 8e-133 68
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-141 67
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-142 67
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-142 67
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-139 67
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-127 67
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-139 66
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 6e-139 66
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-141 66
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-130 64
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-130 64
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-135 64
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-96 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-83 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 6e-83 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-82 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-82 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-83 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-85 50
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-84 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-84 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-84 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-85 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-85 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-85 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-83 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-83 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-86 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-77 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-77 46
escN AAK26715.1 EscN Virulence LEE Protein 1e-78 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 5e-78 46
escN AAL57542.1 EscN Virulence LEE Protein 1e-78 46
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-77 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-78 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-79 46
escN AAC31513.1 L0034 Virulence LEE Protein 9e-78 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 9e-78 46
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-78 46
escN AAC38383.1 EscN Virulence LEE Protein 2e-77 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-78 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-78 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-78 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-63 44
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-66 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-65 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-67 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0192 Protein 5e-96 53
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0390 Protein 2e-91 52
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0048 Protein 4e-92 51
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0515 Protein 7e-83 50
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG2144 Protein 7e-83 46
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0812 Protein 5e-78 46
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0730 Protein 9e-78 45
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG1254 Protein 4e-70 44
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG1008 Protein 4e-68 42
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG2459 Protein 1e-69 42
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG1778 Protein 3e-67 42
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG2499 Protein 3e-69 42
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG2331 Protein 6e-68 41
rscN YP_006322227.1 type III secretion ATP synthase RscN VFG0555 Protein 1e-67 41