Gene Information

Name : Q7C_2350 (Q7C_2350)
Accession : YP_006294171.1
Strain : Methylophaga sp. JAM7
Genome accession: NC_017856
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase FliI
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2357062 - 2358471 bp
Length : 1410 bp
Strand : +
Note : PFAM: ATP synthase alpha/beta family, nucleotide-binding domain; TIGRFAM: ATPase FliI/YscN family; flagellar protein export ATPase FliI

DNA sequence :
ATGGCACAACCTGAATTTGGTCTCCGTCGCTGGCATCAGCAGATCCAGGATTATCAACAGCGATTAATGTCATTACAGCC
GGAAGCGGTCATGGCTGTTGAAGGTCGGCTGACACGGATGGTCGGCATGACTTTAGAGGCCGTGGGATTTCAGGCACCTA
TTGGCAGTCGCTGTGAAATTCAGGCTCGCGGACAGCCGCCTGTTGAGGCAGAAGTGGTTGGTTTTCATGACGATGTCTTG
TACCTGATGCCAACGGGTGATATCCGCGGCTTAGTGCCGAATGCCAAAGTCAGACCTATTCAGAATGATTCCCTGATACC
TGTCGGCGAAGCCTTACTGGGGCGTGTTGTTGATGGTGCGGGCAAACCACTGGATGGCAAAGGCGCAATTAAGGTTAAGG
ATCGTGTGCCACTCCATGGTCAACCGGTCAATCCACTGAGCAGAGCGCCGGTAAGACAACATCTCGATGTCGGGGTACGG
GCGATAAATGCCTTGCTCAGTGTCGGACGGGGGCAGCGGATGGGGCTGTTTGCCGGAAGCGGCGTTGGCAAAAGTGTGCT
GCTCGGCATGATGACCCGATTTACCGAAGCGGATGTGATTGTGGTGGGGCTGATTGGTGAGCGTGGTCGGGAAGTTAAAG
AATTTATTGAAGATATTTTAGGTGAGCAAGGACTGAAACGTGCGGTGGTGGTTGCTTCTCCAGCAGATCATTCTCCTTTG
ATGCGGCTGCATGGCGCGATGTTGGCGACCAGTATCGCTGAGTATTTCAGGGACCAAGGTAAGCAAGTCCTGCTGTTGAT
GGATTCCTTGACTCGTTATGCGCAGGCGCAACGCGAAATTGCCTTGGCTATTGGTGAACCACCAGCAACCAAGGGCTATC
CACCCTCAGTTTTTTCATTATTGCCGCAACTAGTCGAGCGCGCAGGAAATGCCGGTGAAGGACAAGGGGGTATAACTGCC
ATTTATACCGTGCTGGTTGAGGGGGATGATCAGCAGGATCCGGTTGCCGATTCGGCGCGAGCGATTCTGGATGGTCATAT
CGTGTTATCCAGGCAACTGGCTGAGTCCGGCGTGTATCCAGCCATTGATGTCGAGGCATCGATCAGTCGGGTTATGCCTC
AGGTGACCAGTCGTGAGCAATTGCAACAAGCGCAATTATTTAAACAGATTTATGCAACCTACCGGCAGAATCAGGATTTG
ATTAATATCGGAGCCTACAGCGCTGGCAGTGACCCGCAAATTGATCACGCGATTGAAATGTATCCAAAGTTACGCAAACT
CTTGAAGCAGGACATGCATCAAGCGCATGATTGGCAGGCAAGTCTGTCTACGTTACAGCAAACGCTGCAGACAAATACGG
CAGGGGGACAAACTGGTTCGATTGGTCAGCCTGTCACGGCAAGAAAATAA

Protein sequence :
MAQPEFGLRRWHQQIQDYQQRLMSLQPEAVMAVEGRLTRMVGMTLEAVGFQAPIGSRCEIQARGQPPVEAEVVGFHDDVL
YLMPTGDIRGLVPNAKVRPIQNDSLIPVGEALLGRVVDGAGKPLDGKGAIKVKDRVPLHGQPVNPLSRAPVRQHLDVGVR
AINALLSVGRGQRMGLFAGSGVGKSVLLGMMTRFTEADVIVVGLIGERGREVKEFIEDILGEQGLKRAVVVASPADHSPL
MRLHGAMLATSIAEYFRDQGKQVLLLMDSLTRYAQAQREIALAIGEPPATKGYPPSVFSLLPQLVERAGNAGEGQGGITA
IYTVLVEGDDQQDPVADSARAILDGHIVLSRQLAESGVYPAIDVEASISRVMPQVTSREQLQQAQLFKQIYATYRQNQDL
INIGAYSAGSDPQIDHAIEMYPKLRKLLKQDMHQAHDWQASLSTLQQTLQTNTAGGQTGSIGQPVTARK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-83 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-79 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-79 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 5e-78 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-78 50
escN AAK26715.1 EscN Virulence LEE Protein 1e-76 50
escN AAL57542.1 EscN Virulence LEE Protein 1e-76 50
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-77 50
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 6e-77 50
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-88 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-88 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-88 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-89 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-89 49
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-78 49
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-79 49
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-78 49
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-78 49
escN AAC38383.1 EscN Virulence LEE Protein 1e-78 49
escN AAC31513.1 L0034 Virulence LEE Protein 8e-79 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-89 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-89 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-89 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-86 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-86 48
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-78 48
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-74 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-74 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-77 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-73 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-73 45
escN CAI43874.1 EscN protein Virulence LEE Protein 8e-78 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-77 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-74 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-74 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-74 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-70 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-70 45
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-72 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-72 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-72 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 7e-65 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-65 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-65 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 7e-65 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-72 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-64 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 6e-65 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG1254 Protein 2e-134 65
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG2331 Protein 2e-115 62
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG2499 Protein 1e-124 58
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0515 Protein 4e-79 50
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG2144 Protein 2e-93 49
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0192 Protein 4e-91 49
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0390 Protein 4e-86 49
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0730 Protein 6e-79 49
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0812 Protein 5e-79 49
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0048 Protein 6e-86 46
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG0555 Protein 3e-65 44
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG1008 Protein 8e-64 42
Q7C_2350 YP_006294171.1 flagellum-specific ATP synthase FliI VFG2459 Protein 5e-65 42