Gene Information

Name : nrps2B (ACPL_6156)
Accession : YP_006269109.1
Strain : Actinoplanes sp. SE50/110
Genome accession: NC_017803
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 6766816 - 6768450 bp
Length : 1635 bp
Strand : -
Note : Peptide arylation enzymes

DNA sequence : 
ATGGACAGCCGCGTGCTCGAAAACTGTGTTCCCTGGCCCGACGAGATCACCGTCGCCTATCGGCAGGCGGGTTACTGGAC
CGACGAGGTCCTCGGTCGATGGCCGGCCGGTGACCCCGGCCGCGCCGGCCGCCCCGCCGTGGTGACCGCCGGGCGCACGA
TGACCTACGCCGAACTCGACCGGGCGGCCGACCGCCGCGCGGCCGGCCTGCTCGACCGCGGCATCGGACGTGCCGACCGG
GTCGTCGTCCAGCTACCCAACGACGTCGACCTGGTCGTGTGCATCCTCGCGCTGCTGCGGATCGGCGCACTGCCGGTCTT
CGCGCTGCCCGCACACCGCACCATGGAGATCGTCGACCTGGTCGAGGCGTCCGGCGCCCGGGCGTATCTGTGCGCCGACG
TGCTCCTCGGTCACGACTTCCGGGCCATGGCGGCCGAGGTTGTGCGGCGGACCTCGACGCTCACCGAGGTCTTCGTGGCC
GGTGCGCCCGGCGACTTCACCGCGCTCGCCGACGTCGACGCCGAGCCACGGCCGATCACCCCGCCGGCCCCCGGCGACGT
GGCGATGTTCCTGCTCTCCGGCGGCACCACGGGCAAGCCCAAGCTGATCCCGCGCACCCATGCGGATTACGCGTACCAAC
TGCGCGCCAGCGCCGAGGTCTGCGGCGTGGGCCCGGACAGCCGGGTGCTGGCGGCGTTGCCCGTCGGACACAACTTCCCG
CTGGGCTGCCCGGGCGTGCTCGGGGTGCTGCGCGCCGGCGGCGTGGCCGTGCTGGCGCCGTCGCCGGCGCCGGACGACTG
CTTCCCGCTGATCGCGTCGGCGGGCGTGACCCTGACCGCCCTGGTGCCGCCGATGGTGCCGCTCTGGCTGGAAGCCGTCG
AGTGGCTGCCGGACGACCTCTCCTCGCTGGAGGTGCTCCAGGTCGGCGGCGCGCGCCTGGATCCGGTGACGGCGGGCCGG
GTCGTCGGCGGCCTCGGCTGCCGCCTGCAGCAGGTCTTCGGGATGGCCGAGGGGCTGCTCAACTTCACGCGCCTCGACGA
CCCGCCGGAGCTGGTGCTCACCACCCAGGGCCGCCCGCTCTCCCCGGCCGACGAGATCAGGATCGTCGCGCCGGACGGCA
CGGAGGTCGCCCCGGGCGAGCCCGGCGAGCTGCTCACCCGGGGGCCGTACACACTGCGCGGCTACTACCGGGCGCCCGAG
GTCGACCGGGTCCGCTTCACCGAGGACGGGTTCTTCCGGACCGGCGACGTCGTCCGGGCCCGGCGCACCGGTCACCTGGA
GGTGGTCGGACGGATCAACGACGTCGTCAACCGGGGCGGCGAGAAGGTGCCCGCGCAGGAGCTGGAGGAGCTGCTGACCA
CGCTGCCGGCGGTGGCCGGCGCCGCCGTCGTGGCCGTGCCCGACCCGGACATGGGCGAGCTGACCTGCGCCTGCATCGTC
GCCCGGGAGTCGCAGGGCGCACCGGAGCTGCACGAGATCCGGGGCGCCCTGAGCGAGCGCGGGGTGGCCGCCTTCAAGCT
GCCGGACCGCCTCGTCGTGCTGGACCGCTTGCCGCTCACCGCCGTCGGCAAGATCGACAAGAAGGCCCTCGCGGCGCGCG
CCGCCGTGCAGACGACCGGAGGAGGAGCCCGATGA

Protein sequence : 
MDSRVLENCVPWPDEITVAYRQAGYWTDEVLGRWPAGDPGRAGRPAVVTAGRTMTYAELDRAADRRAAGLLDRGIGRADR
VVVQLPNDVDLVVCILALLRIGALPVFALPAHRTMEIVDLVEASGARAYLCADVLLGHDFRAMAAEVVRRTSTLTEVFVA
GAPGDFTALADVDAEPRPITPPAPGDVAMFLLSGGTTGKPKLIPRTHADYAYQLRASAEVCGVGPDSRVLAALPVGHNFP
LGCPGVLGVLRAGGVAVLAPSPAPDDCFPLIASAGVTLTALVPPMVPLWLEAVEWLPDDLSSLEVLQVGGARLDPVTAGR
VVGGLGCRLQQVFGMAEGLLNFTRLDDPPELVLTTQGRPLSPADEIRIVAPDGTEVAPGEPGELLTRGPYTLRGYYRAPE
VDRVRFTEDGFFRTGDVVRARRTGHLEVVGRINDVVNRGGEKVPAQELEELLTTLPAVAGAAVVAVPDPDMGELTCACIV
ARESQGAPELHEIRGALSERGVAAFKLPDRLVVLDRLPLTAVGKIDKKALAARAAVQTTGGGAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-99 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-98 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-99 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-99 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-99 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-99 50
ybtE CAA21394.1 - Virulence HPI Protein 7e-99 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 6e-99 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 7e-99 50
irp2E YP_005163485.1 siderophore biosynthetic protein Virulence Not named Protein 9e-71 43
irp2E YP_005134497.1 siderophore biosynthetic protein Virulence Not named Protein 6e-71 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
nrps2B YP_006269109.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 8e-98 48
nrps2B YP_006269109.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 9e-95 48
nrps2B YP_006269109.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 2e-87 43