Gene Information

Name : S70_09815 (S70_09815)
Accession : YP_006216514.1
Strain : Providencia stuartii MRSN 2154
Genome accession: NC_017731
Putative virulence/resistance : Virulence
Product : ATP synthase SpaL
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2101460 - 2102752 bp
Length : 1293 bp
Strand : -
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGAAGCTTTTTGATTCTTGCGCGCACCCTGCGCGCATTCATGGCTGTTTAATGGAAGCCCCTTTGCAGGGAGTTTTTAT
TGGTGAGATCTGTGTTGTTGAACGCTCGATAACGTCAAAAGAGCGCATAGCAAAAGCACAAGTCGTGGGGTTTAAAGAGG
GCTTAACTGTGCTCAGCTTAATTGGTCGAGCACAAGGGTTAACGCGTGATATGGTGATCCGCCCAACGGGCCTACCTTTC
GTGTTTGAAATGCATGAGCAGCTTGCCGGTAAAATTTTTGATGCGGCGGGTGAGCAGGTTGGTGTGCTCGGTCATACAGC
CGAAACAGCGTTCTGCCCTGACTCAAGACTAATGCGTATTGATAGTCCACCCGCGAGCGTGACATCTCGGCGTCCGATTG
ATGAACCACTTATCACCGGTATCCGTGCGATAGATGGCTTATTAACCTGTGGTCTTGGGCAGCGTATTGGTATTTTTGCT
GCGGCAGGAAGTGGCAAAACTTCATTAATGAATATGATTATTAACCACGCGAAGGCGGATATCCATGTTGTTGCTTTAAT
CGGTGAGCGTGGCCGAGAGGTTATTGAATTTATTGAGGAGTTACAAGCCTCTCCTCATGCAGCCCAAACGGTGCTTATTT
ATGCCACTTCCGATAGCCCACCGATAGAGCGGTGTAATGCGGCGTTGCTTGCTACGACAATCGCGGAATATTTCCGTGAC
CGTAATCGACATGTCTTGTTATTTGTCGATTCAATGACACGTTACGCCAGAGCATTACGTGATGTTGCTTTGGCGGCGGG
GGAACTTCCAGCAAGACGAGGCTACCCAGCGTCGGTGTTTGAGCAACTGCCTATGTTGCTTGAGCGCCCAGGTCGTATGC
AGCAAGGCTCAATTACCGCCTTTTATACCGTATTGCTAGAAAGTGAAGAAGAGTCAGACCCTATTGGTGATGAAATTCGC
TCGATTCTTGATGGGCATATTTATCTTAGCCATAAGTTGGCGGGAAAGGGGCATTACCCAGCGATTGATATTTTGCACAG
CATTAGTCGTGTATTCCAAAAAGTCACGACGGATACACATCGCCAAGCCGCAACAAAAGTGCGTGATCGTCTATCTAGGC
TAGAACAAATTCAGCTGTATTTGGAGCTTGGCGAATACCAACGTGGGGAAAGTGAAGACAACGACAATGCTTTGGATAAA
AAAGAAGATATTGAACAGTTCTTGCAACAAAAGATGGATGAAGCTGAGCATTTCGAAATTGTATTAAACCGCCTCGACTA
TTTGGCTTCCTGA

Protein sequence :
MKLFDSCAHPARIHGCLMEAPLQGVFIGEICVVERSITSKERIAKAQVVGFKEGLTVLSLIGRAQGLTRDMVIRPTGLPF
VFEMHEQLAGKIFDAAGEQVGVLGHTAETAFCPDSRLMRIDSPPASVTSRRPIDEPLITGIRAIDGLLTCGLGQRIGIFA
AAGSGKTSLMNMIINHAKADIHVVALIGERGREVIEFIEELQASPHAAQTVLIYATSDSPPIERCNAALLATTIAEYFRD
RNRHVLLFVDSMTRYARALRDVALAAGELPARRGYPASVFEQLPMLLERPGRMQQGSITAFYTVLLESEEESDPIGDEIR
SILDGHIYLSHKLAGKGHYPAIDILHSISRVFQKVTTDTHRQAATKVRDRLSRLEQIQLYLELGEYQRGESEDNDNALDK
KEDIEQFLQQKMDEAEHFEIVLNRLDYLAS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-112 58
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-109 57
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-109 57
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-109 57
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-109 57
invC AAS66861.1 InvC Not tested SSR-2 Protein 4e-105 54
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 3e-111 54
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-71 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-71 48
escN CAI43874.1 EscN protein Virulence LEE Protein 4e-70 48
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-68 48
escN AAK26715.1 EscN Virulence LEE Protein 1e-69 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-69 48
escN AAL57542.1 EscN Virulence LEE Protein 1e-69 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-69 48
escN AAC38383.1 EscN Virulence LEE Protein 1e-69 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-69 48
escN AAC31513.1 L0034 Virulence LEE Protein 1e-69 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 8e-70 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-69 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 5e-70 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-70 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 5e-75 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-75 45
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-75 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-74 45
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-74 45
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-79 45
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-79 45
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-82 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-78 44
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-77 44
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 6e-77 44
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-79 43
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-79 43
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-79 43
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-59 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-68 43
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-79 42
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-79 42
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-62 41
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-62 41
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 4e-64 41
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-64 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-61 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-64 41
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-64 41
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 4e-65 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-61 41
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 4e-69 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
S70_09815 YP_006216514.1 ATP synthase SpaL VFG1778 Protein 1e-160 77
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0555 Protein 9e-110 57
S70_09815 YP_006216514.1 ATP synthase SpaL VFG2459 Protein 5e-117 56
S70_09815 YP_006216514.1 ATP synthase SpaL VFG1008 Protein 1e-97 51
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0730 Protein 6e-70 48
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0812 Protein 6e-70 48
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0192 Protein 4e-87 46
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0390 Protein 5e-83 45
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0515 Protein 2e-74 45
S70_09815 YP_006216514.1 ATP synthase SpaL VFG2331 Protein 4e-59 44
S70_09815 YP_006216514.1 ATP synthase SpaL VFG0048 Protein 5e-77 43
S70_09815 YP_006216514.1 ATP synthase SpaL VFG2144 Protein 4e-72 42
S70_09815 YP_006216514.1 ATP synthase SpaL VFG1254 Protein 8e-66 42
S70_09815 YP_006216514.1 ATP synthase SpaL VFG2499 Protein 1e-62 41