Gene Information

Name : i02_0334 (i02_0334)
Accession : YP_006147554.1
Strain : Escherichia coli clone D i2
Genome accession: NC_017651
Putative virulence/resistance : Virulence
Product : Pic serine protease
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 330422 - 334537 bp
Length : 4116 bp
Strand : -
Note : -

DNA sequence :
GTGAATAAAGTTTATTCTCTTAAATATTGCCCTGTCACCGGGGGACTTATTGTTGTCTCTGAACTTGCCAGCAGGGTAAT
AAAAAAGACATGCCGAAGATTAACGCATATTCTTCTGGCTGGCATTCCAGCAGTCTATCTGTATTACCCTCAGATATCCC
AGGCGGGCATTGTCCGTTCCGATATTGCCTATCAGATTTATCGTGATTTCGCTGAAAACAAAGGGCTTTTTGTACCTGGT
GCCACAGATATTCCGGTATATGATAAGGACGGAAAACTTGTGGGGAGACTGGATAAAGCCCCAATGGCCGATTTCAGCAG
TGTGAGCTCAAATGGCGTTGCTACGCTTGTATCACCTCAGTATATCGTCAGCGTAAAGCATAACGGAGGATATCAGAGTG
TGAGCTTTGGTAATGGGAAAAATACATATTCCCTTGTTGACCGTAATAACCACTCTTCTGTTGACTTCCATGCTCCACGT
CTGAATAAACTGGTTACAGAAGTTATTCCCTCAGCGATAACATCAGAAGGAACCAAAGCCAATGCTTATAAAGACACTGA
ACGTTACACCGCTTTTTATCGGGTGGGTAGTGGTACGCAGTACACTAAGGACAAGGACGGAAATTTAGTTAAGGTTGCCG
GCGGATATGCTTTTAAAACAGGAGGAACCACAGGAGTTCCTCTGATATCTGATGCAACAATAGTCTCTAATCCCGGGCAA
ACCTATAATCCTGTAAACGGACCTTTACCTGACTATGGTGCCCCTGGGGACAGTGGTTCTCCTTTGTTTGCTTATGATGA
ACAACAAAAAAAATGGGTTATTGTTGCTGTATTAAGAGCATATGCAGGTATTAATGGTGCTACGAACTGGTGGAATGTCA
TACCAACAGATTATCTGAACCAGGTTATGCAGGACGATTTCGATGCCCCCGTGGACTTTGTTTCCGGACTGCCCCCCCTG
AACTGGACATACGACAAAACATCAGGCACAGGCACCCTGAGCCAGGGCAGTAAAAACTGGACCATGCACGGGCAGAAAGA
CAATGATCTCAATGCCGGTAAAAATCTGGTATTCAGCGGGCAGAACGGTGCAATTGTCCTGAAAGACAGTGTGACTCAGG
GGGCCGGTTATCTCGAATTTAAAGACAGTTACACCGTATCTGCTGAATCCGGAAAAACCTGGACGGGTGCCGGCATTATT
ACTGACAAGGGGACGAATGTGACCTGGAAGGTCAACGGGGTTGCCGGTGACAACCTGCATAAATTGGGGGAAGGAACCCT
GACCATAAACGGAACAGGTGTAAACCCGGGAGGACTGAAAACGGGAGACGGTACCGTTGTACTTAACCAGCAGGCAGACA
CTGCAGGTAATGTTCAGGCCTTCAGTTCCGTGAACCTCGCCAGCGGACGACCGACCGTGGTGCTCGGAGATGCCCGTCAG
GTCAATCCGGATAACATTTCATGGGGATACCGGGGAGGTAAGCTTGACCTTAATGGTAATGCCGTTACCTTCACCCGACT
GCAGGCTGCCGATTACGGGGCGGTGATTACAAATAATGCACAGCAAAAATCCCGGCTTTTACTGGATCTTAAGGCTCAGG
ATACAAATGTCAGTGTTCCGATTGGCAGTATATCCCCCTTTGGCGGTACCGGCACACCGGGAAACCTATACAGCATGATA
CTCAACGGCCAGACCCGCTTCTATATTCTGAAATCTGCCAGCTATGGTAACACCCTGTGGGGGAACAGCCTGAATGACCC
GGCTCAGTGGGAATTTGTTGGCACGGACAAAAACAAAGCAGTTCAGACAGTAAAAGACCGGATCCTGGCCGGGCGGGCAA
AACAACCCGTTATCTTTCATGGTCAGCTGACCGGGAATATGGATGTCACCATTCCACAGCTGCCGGGGGGAAGAAAGGTC
ATCCTTGATGGTAGCGTGAACCTGCCGGAAGGTACCCTGAGTGAGGACAGTGGCACCCTGATATTCCAGGGGCATCCGGT
TATCCACGCCTCCGTCAGTGGCAGTGCGCCGGTCAGCCTGAACCAGAAAGACTGGGAAAACCGCCAGTTCATAATGAAAA
CACTGTCGCTGAAAGATGCTGACTTCCATCTTTCACGTAACGCCTCGCTGAACAGTGACATTAAGTCGGATAACAGCCAT
ATCACACTGGGAAGTGACAGGGTATTTGTGGATAAAAATGACGGAACAGGAAATTATGTCATTCTGGAGGAAGGTACCTC
TGTCCCGGACACCGTGAATGACAGGAGCCAGTATGAAGGGAATATTACGCTGGACCATAACTCAACCCTGGATATCGGCA
GCCGGTTCACCGGAGGGATTGAAGCTTATGACAGTGCCGTCAGTATCACCTCTCCGGACGTCCTGTTAACAGCCCCGGGT
GCTTTTGCCGGCAGTTCACTGACAGTGCATGATGGCGGTCATCTTACAGCACTGAACGGTCTTTTCAGCGACGGGCATAT
TCAGGCCGGTAAGAACAGCAAAATCACCCTGAGCGGTACACCGGTTAAAGATACGGCTAACCAGTATGCCCCTGCTGTAT
ATCTGACGGACGGATATGACCTGACCGGCGATAACGCAACACTGGAAATTACCCGTGGAGCACATGCTTCCGGTGATATT
CATGCCTCTGCGGCATCAACAGTTACCATCGGGTCTGACACGCCGGCAGAACTGGCTTCTGCGGAAACGACTGCATCGGC
GTTTGCCGGCAGTCTTCTTGAGGGCTATAACGCAGCATTCAATGGTGCCATAACCGGTGGCAGGGCTGATGTCAGTATGC
ATAATGCACTGTGGACTCTGGGTGGGGACTCCGCCATCCACACTCTTACCGTCAGAAACAGCCGTATCAGTTCTGAAGGA
GACCGTACTTTCCGTACCCTGACGGTGAATAAACTGGATGCAACAGGCAGTGATTTTGTTCTGCGTACGGACCTGAAAAA
TGCCGATAAAATTAATGTGACTGAAAAAGCCACGGGTTCAGATAACAGCCTGAACGTCAGCTTTATGAAGGATCCGGCTC
AGGGACAGTCCCTGAATATTCCTCTGGTCACGGCACCGGCGGGAACTTCAGCAGAGATGTTTAAGGCCGGCACCCGGATG
ATAGGTTTCAGTCGGGTGACTCCAACCCTGCATGTTGACACCAGTGGTGGCAATACGAAGTGGATACTGGACGGTTTTAA
AGCGGAGGCTGATAAAGCCGCTGCCGCGAAGGCTGACAGTTTCATGAATGCCGGGTATAAAAACTTCATGACGGAAGTCA
ACAATCTGAACAAACGTATGGGTGACCTGCGTGACACAAACGGTGATGCCGGAGCCTGGGCGCGCATCATGAGTGGTGCC
GGTTCTGCAGACGGTGGTTACAGTGATAATTACACCCATGTTCAGGTCGGCTTTGACAAAAAACATGAACTGGACGGAGT
GGACCTGTTTACCGGTGTCACGATGACCTATACCGACAGCAGTGCAGACAGCCATGCGTTCAGCGGCAAGACGAAATCGG
TGGGGGGCGGTCTGTATGCTTCAGCATTGTTTGAGTCCGGTGCCTATATCGATTTGATTGGTAAATATATTCACCATGAC
AATGATTACACAGGTAACTTTGCCGGTCTGGGAACGAAACACTACAACACCCATTCCTGGTATGCCGGTGCTGAAACGGG
TTACCGCTATCACCTGACAGAGGAAACGTTCATTGAGCCGCAGGCTGAACTGGTTTACGGCGCCGTGTCCGGGAAAACAT
TCCGCTGGAAAGACGGTGATATGGACCTGAGCATGAAGAACAGGGACTTCAGTCCGCTGATTGGAAGAACAGGGATTGAA
CTGGGCAAGACCTTCAGTGGTAAGGACTGGAGTGTGACGGCCCGTGCCGGGACCAGCTGGCAGTTTGACCTACTGAATAA
TGGTGAGACGGTACTGCGTGATGCGTCCGGGGAGAAACGGATAAAAGGCGAGAAAGACAGCCGGATGCTGTTTAATGTTG
GTATGAATGCGCAGATAAAGGACAATATGCGCTTTGGTCTGGAGTTTGAGAAGTCAGCCTTTGGTAAATATAACGTGGAT
AATGCGGTAAACGCAAATTTCCGGTATATGTTCTGA

Protein sequence :
MNKVYSLKYCPVTGGLIVVSELASRVIKKTCRRLTHILLAGIPAVYLYYPQISQAGIVRSDIAYQIYRDFAENKGLFVPG
ATDIPVYDKDGKLVGRLDKAPMADFSSVSSNGVATLVSPQYIVSVKHNGGYQSVSFGNGKNTYSLVDRNNHSSVDFHAPR
LNKLVTEVIPSAITSEGTKANAYKDTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAFKTGGTTGVPLISDATIVSNPGQ
TYNPVNGPLPDYGAPGDSGSPLFAYDEQQKKWVIVAVLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLPPL
NWTYDKTSGTGTLSQGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIVLKDSVTQGAGYLEFKDSYTVSAESGKTWTGAGII
TDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGTVVLNQQADTAGNVQAFSSVNLASGRPTVVLGDARQ
VNPDNISWGYRGGKLDLNGNAVTFTRLQAADYGAVITNNAQQKSRLLLDLKAQDTNVSVPIGSISPFGGTGTPGNLYSMI
LNGQTRFYILKSASYGNTLWGNSLNDPAQWEFVGTDKNKAVQTVKDRILAGRAKQPVIFHGQLTGNMDVTIPQLPGGRKV
ILDGSVNLPEGTLSEDSGTLIFQGHPVIHASVSGSAPVSLNQKDWENRQFIMKTLSLKDADFHLSRNASLNSDIKSDNSH
ITLGSDRVFVDKNDGTGNYVILEEGTSVPDTVNDRSQYEGNITLDHNSTLDIGSRFTGGIEAYDSAVSITSPDVLLTAPG
AFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNSKITLSGTPVKDTANQYAPAVYLTDGYDLTGDNATLEITRGAHASGDI
HASAASTVTIGSDTPAELASAETTASAFAGSLLEGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHTLTVRNSRISSEG
DRTFRTLTVNKLDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMKDPAQGQSLNIPLVTAPAGTSAEMFKAGTRM
IGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYKNFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGA
GSADGGYSDNYTHVQVGFDKKHELDGVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHD
NDYTGNFAGLGTKHYNTHSWYAGAETGYRYHLTEETFIEPQAELVYGAVSGKTFRWKDGDMDLSMKNRDFSPLIGRTGIE
LGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVD
NAVNANFRYMF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
she AAB58244.1 mucinase Virulence SHI-1 Protein 0.0 97
pic NP_838464.1 serine protease precurser Virulence SHI-1 Protein 0.0 97
pic NP_708747.3 serine protease Not tested SHI-1 Protein 0.0 97
pic AAK00464.1 Pic Virulence SHI-1 Protein 0.0 97
unnamed CAC39286.1 hypothetical protein Not tested LPA Protein 0.0 53
unnamed CAD66214.1 putative hemoglobin protease Not tested PAI III 536 Protein 0.0 48
vat YP_851472.1 vacuolating autotransporter Not tested PAI III APEC-O1 Protein 0.0 48
vat AAO21903.1 vacuolating autotransporter toxin Virulence Not named Protein 0.0 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
i02_0334 YP_006147554.1 Pic serine protease VFG0903 Protein 0.0 100
i02_0334 YP_006147554.1 Pic serine protease VFG0635 Protein 0.0 97
i02_0334 YP_006147554.1 Pic serine protease VFG0861 Protein 0.0 97
i02_0334 YP_006147554.1 Pic serine protease VFG1689 Protein 0.0 48
i02_0334 YP_006147554.1 Pic serine protease VFG0904 Protein 0.0 48