Gene Information

Name : Sput200_3755 (Sput200_3755)
Accession : YP_006011595.1
Strain : Shewanella putrefaciens 200
Genome accession: NC_017566
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 4255778 - 4257430 bp
Length : 1653 bp
Strand : -
Note : KEGG: shw:Sputw3181_3208 mercuric reductase; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; pyridine nucleotide-disulphide oxidoreductase dimerisation region

DNA sequence : 
ATGCTCTTGTTATCGATAAAAGGGATGACCTGCCCAAGCTGTGTCTCCCATATTAAAGATGCGCTCGATGCGACCGAAGG
AGTGAATAACGTTGAGATCTCCTATGCAAATGCCAGCGCAGCGGTCACCACAAACGGGAAGGTAAGTGTGGCGGATCTCG
TCAAGGCGGTTAAAACTCTGGGTTATGCTGCACAAGTCAATCCAGAACCTCAAACTTGTTTTGACAACACTAATCTCAGC
AATACAGAAAATATCAGCCCACAACAGGTGGTGATCATTGGCAGTGGCTCTGGTGCTTTTGCCTGTGCGATTAAAGCTGC
TGACGGAGGAGCTAAGGTTACGCTCATCGAAGGTGCGGATGTTATTGGCGGCTGCTGTGTCAATGTCGGCTGTGTCCCGT
CCAAGATCCTGATCCGCGCCGCACAACTGGCGCAGCAACAACGAAACAATCCTTTTATCGGATTGGCAAATCATTCACCC
CAGCTCAATCGCGCACTGTTGGCTGAGCAACAAACAGCGCTTGTCGAAGAATTACGCGTGGCAAAATACCAAAATATTCT
GGATATCAATCCGGCACTGAGTTTGATAAAAGGTTGGGCGACTTTCAAAAACGCGAACACTTTGATTGTCAGCAAAAGTG
ATGGCTCCCAACAAGAAATACACGCTGACAAGGTGCTGATTGCTACCGGTTCGAGCCCGACCATCCCACCTATTGAAGGT
CTGAGCGAAACCCCGTACTGGACTTCTTCTGAAGCGCTGTTTGCGACTGAACTACCAGAACATCTGGTCATCATTGGATC
TTCTGTCGTTGCGCTGGAAATTGCTCAGGCTTATCGCCGTTTAGGCAGTGACGTTACCCTATTGGCCAGACACACGCTTC
TTTATCGCGATGAGCCGTTACTCGGTGAAAAATTAACAGACTGCTTTGAGAATGAAGGTATCAGGGTTCTAAACCATACC
GAGGCGTCACATGTTTCCCATGACGGACGGCAGTTTACCTTGAAAACCAATGCAGGCACTTTACGCTGTGACAAGCTTCT
AATATGCACAGGGCGTCATGCAAATACAGCCAGTCTTAACCTCGATGCAGTTGGGATCAGGACAGACAAACACGGGGCGA
TCATCGTTAACGAGATGATGGAAACAAGCGTTCCAGGGGTTTATGCCGCAGGTGATTGTTCCACTATGCCGCAGTTTGTA
TATGTCGCTGCGGCGGCGGGTAGCCGCGCAGGAATGAATATGACCGGTGGCGAGAGTAAACTTGACCTATCCTCGATGCC
GGCGGTCATTTTTACCGATCCGCAGGTTGCAACCGTCGGACTGACGGAAGTTGAGGCCAGACAGCAGGGTATTCACACGG
ACAGCCGGGTACTCGACATGAAAAATGTACCCAGAGCACTGGCCAATTTCGAAACTGACGGCTTTATCAAGCTCGTGATT
GAAAAGACCACAGGAAGACTGATTGGTGCACAACTTTTGGCCCATGAAGGCGGAGAAATGATCCAAACTGCAGCTCTAGC
TATCCGCAATCGGATGACTATCACTGAGTTAGCTGACCAGTTGTTTCCATATTTGACCATGGTTGAAGGGATCAAGCTCT
GCGCTCAAACCTTTCACAAAGATGTGAATGAGTTGTCCTGCTGTGCTGGATGA

Protein sequence : 
MLLLSIKGMTCPSCVSHIKDALDATEGVNNVEISYANASAAVTTNGKVSVADLVKAVKTLGYAAQVNPEPQTCFDNTNLS
NTENISPQQVVIIGSGSGAFACAIKAADGGAKVTLIEGADVIGGCCVNVGCVPSKILIRAAQLAQQQRNNPFIGLANHSP
QLNRALLAEQQTALVEELRVAKYQNILDINPALSLIKGWATFKNANTLIVSKSDGSQQEIHADKVLIATGSSPTIPPIEG
LSETPYWTSSEALFATELPEHLVIIGSSVVALEIAQAYRRLGSDVTLLARHTLLYRDEPLLGEKLTDCFENEGIRVLNHT
EASHVSHDGRQFTLKTNAGTLRCDKLLICTGRHANTASLNLDAVGIRTDKHGAIIVNEMMETSVPGVYAAGDCSTMPQFV
YVAAAAGSRAGMNMTGGESKLDLSSMPAVIFTDPQVATVGLTEVEARQQGIHTDSRVLDMKNVPRALANFETDGFIKLVI
EKTTGRLIGAQLLAHEGGEMIQTAALAIRNRMTITELADQLFPYLTMVEGIKLCAQTFHKDVNELSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA AGK07079.1 MerA Not tested SGI1 Protein 1e-130 56
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 9e-130 56
merA ABQ57371.1 MerA Not tested SGI1 Protein 1e-130 56
merA AGK07021.1 MerA Not tested SGI1 Protein 1e-130 56
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 3e-132 56
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 2e-129 56
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 5e-127 55
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 3e-127 55
merA AFG30120.1 MerA Not tested PAGI-2 Protein 3e-127 55
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 1e-124 53

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Sput200_3755 YP_006011595.1 mercuric reductase BAC0652 Protein 2e-128 55
Sput200_3755 YP_006011595.1 mercuric reductase BAC0653 Protein 4e-128 55
Sput200_3755 YP_006011595.1 mercuric reductase BAC0650 Protein 8e-126 54