Gene Information

Name : topB (PAJ_1424)
Accession : YP_005934300.1
Strain : Pantoea ananatis AJ13355
Genome accession: NC_017531
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III TopB
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 1719631 - 1721547 bp
Length : 1917 bp
Strand : -
Note : similar to Pantoea sp. At- 9b, DNA topoisomerase III (NCBI: ZP_05729421.1) COG: DNA replication, recombination and repair subcellular localization as predicted by Psort 2.0: cytoplasmic

DNA sequence :
ATGCGTTTGTTTATTGCAGAGAAACCCAGCCTTGGACGGGCCATTGCCGATGTACTGCCTAAACCGCACCGGCGTGGTGA
GGGCTTTATCGCCTGCGGCAATGACCAGATAGTCACCTGGTGCGTGGGGCATTTACTGGAGCAGGCGCAGCCTGATGTGT
ACGACAGCCGCTATGCGCGCTGGTCGCTGGTCGATCTGCCGATTATTCCTGAAAAATGGCGTTTGCAGCCGCGTCCTTCG
GTGGCAAAACAACTCAAAGTGGTGGAAGGCTTACTGGCCCAGGCCGATGAAGTGGTGCACGCCGGCGACCCGGACAGGGA
AGGCCAGCTGCTTGTGGATGAAGTGCTGGATTACCTTCAGCTTCCCGCTGAGAAACGCAGTAAAGTGCAGCGCTGTCTGA
TTAACGACCTCAACCCACAGGCTGTGGAGCGGGCTATCAACCGGCTGCGTGAGAATCGCGAATTTATTCCCCTCTGCGTG
TCCGCGCTGGCGCGAGCACGTGCCGACTGGCTTTACGGTATTAATATGACCCGCGCCTGGACGCTGCTGGGCCGGAATGC
GGGCTATGATGGCGTGTTGTCCGTTGGACGCGTACAAACGCCGGTGTTGGGGCTGGTGGTGCGACGGGATGAAGAGATCG
CGAATTTCGTCCCGAAGGATTACTTTGAAGTGAAAGCGCACATCGTCACGCCGGATGAGAGCCGGTTTACCGCGAACTGG
ATCCCCAGTGAAGCCTGTGAACCCTGGCAGGATGAAGAAGGTCGCCTGCTGAAACGCTCACTGGCGGAACATGTGGTGGC
GCGCATCAGCGGTCAGCCCGCTATCGTTACCGCCTACAGTGACAAGCGAGAAAACGACATTGCGCCGTTGCCGTTTTCAC
TCTCCAGCCTGCAAATCGAGGCGGCAAAGCGCTTTGGTTTGAGTGCACAGGCGGTGCTGGACATCTGCCAGCGGTTATAT
GAAACCCACAAGCTGATCACCTATCCACGTTCTGATAGCCGCTACTTACCCGATGAGCATTTTGCCGGCCGTCATGCGGT
GCTCAATGCGATCCAGTCCCATCAACCCGATCTTACTCCACCAGCAGACTTTGATGCAGATCGAAAAAATCGCTGTTGGG
ATGACAAAAAAGTGGATGCGCACCACGCCATTATTCCTACCGCGCGCGCCAGCGCAGTGAAACTTAATGAAAACGAACAG
CAGATTTATGGACTGGTTGCGCGTCAGTATTTGATGCAGTTTTGTCCGGATGCCGTGTTCCGCAAATGCACCATCGATCT
GGATATCGCGGGCGGTAAATTCGTGGCTAAAGCGCGCTTTCTGGCGGAGGCGGGCTGGCGTGCGCTGTTGGGTGGCAAAG
AGCGCGATGAAGAAAACGATGGCATGCCGCTGCCTGTTGTAGCGAAAGACGATGAGCTGCTGTGCGAGCGGGGTGAGGTG
CTGGCGAAACAGACACAACCGCCACGCCACTTTACCGACGCGACCTTGCTTTCGGCCATGACCGGCATTGCGCGCTTTGT
GCAGGATAAAGAATTAAAGAAAGTGCTGCGTGCGACCGATGGTTTAGGAACGGAAGCCACGCGCGCAGGCATTATTGAAT
TACTGTTCCGTCGTTCGTTTTTGCATAAGAAAGGGCGCTATATTCATTCTACTGATGCAGGCAAAGCGCTGATCCACTCG
CTACCGGATCTCGCCGCCAGGCCGGACATGACGGCGCAATGGGAATCAACCCTGACGCGTATCAGTGAAAAGGCCTGTCG
CTATGATGAATTTATGCAACCCCTGGTGCAGACGCTGAGCGGGCTTATTCAGCAGGCGCGACAGCAGCCCTCGGGACAGG
CGTTTCGTGGTTTACCGTCCACCGGCCAAAAACGTCCACCGCGTAAAGCAGGGCGTAAAACGAAGGAAACTGAATAA

Protein sequence :
MRLFIAEKPSLGRAIADVLPKPHRRGEGFIACGNDQIVTWCVGHLLEQAQPDVYDSRYARWSLVDLPIIPEKWRLQPRPS
VAKQLKVVEGLLAQADEVVHAGDPDREGQLLVDEVLDYLQLPAEKRSKVQRCLINDLNPQAVERAINRLRENREFIPLCV
SALARARADWLYGINMTRAWTLLGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIANFVPKDYFEVKAHIVTPDESRFTANW
IPSEACEPWQDEEGRLLKRSLAEHVVARISGQPAIVTAYSDKRENDIAPLPFSLSSLQIEAAKRFGLSAQAVLDICQRLY
ETHKLITYPRSDSRYLPDEHFAGRHAVLNAIQSHQPDLTPPADFDADRKNRCWDDKKVDAHHAIIPTARASAVKLNENEQ
QIYGLVARQYLMQFCPDAVFRKCTIDLDIAGGKFVAKARFLAEAGWRALLGGKERDEENDGMPLPVVAKDDELLCERGEV
LAKQTQPPRHFTDATLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFRRSFLHKKGRYIHSTDAGKALIHS
LPDLAARPDMTAQWESTLTRISEKACRYDEFMQPLVQTLSGLIQQARQQPSGQAFRGLPSTGQKRPPRKAGRKTKETE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 3e-109 42
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 4e-106 41
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 3e-106 41
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 4e-106 41
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 3e-105 41
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 6e-107 41